##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050216_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3924288
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.913615922174927	31.0	31.0	34.0	27.0	34.0
2	30.9923812931161	31.0	31.0	34.0	27.0	34.0
3	31.15577755761045	33.0	31.0	34.0	27.0	34.0
4	34.73735439396905	37.0	35.0	37.0	32.0	37.0
5	34.65538895208507	37.0	35.0	37.0	32.0	37.0
6	34.67419440163413	37.0	35.0	37.0	32.0	37.0
7	34.586131038292805	37.0	35.0	37.0	31.0	37.0
8	34.55898063546814	37.0	35.0	37.0	31.0	37.0
9	36.17289352871145	39.0	37.0	39.0	32.0	39.0
10	36.05846283453202	39.0	37.0	39.0	31.0	39.0
11	36.05487441288713	39.0	37.0	39.0	31.0	39.0
12	35.96875968328522	39.0	37.0	39.0	31.0	39.0
13	35.977587781528776	39.0	37.0	39.0	31.0	39.0
14	37.15340133038146	40.0	37.0	41.0	31.0	41.0
15	37.13101969070568	40.0	37.0	41.0	31.0	41.0
16	37.1009864719409	40.0	37.0	41.0	31.0	41.0
17	37.09388556599312	40.0	37.0	41.0	31.0	41.0
18	37.07413854436779	40.0	37.0	41.0	31.0	41.0
19	37.11447656237259	40.0	37.0	41.0	31.0	41.0
20	37.094198234176496	40.0	37.0	41.0	31.0	41.0
21	37.0206330422232	40.0	37.0	41.0	31.0	41.0
22	36.96071771490777	40.0	37.0	41.0	31.0	41.0
23	36.86992417478024	40.0	37.0	41.0	31.0	41.0
24	36.80675628292317	40.0	37.0	41.0	31.0	41.0
25	36.53111545330006	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8501234083317968
1101	2	0.9534615925047873
1101	3	0.8925949209314297
1101	4	0.8063347414913338
1101	5	0.8826352194081082
1101	6	0.809267470152875
1101	7	0.9127181835871809
1101	8	0.8559996661563787
1101	9	1.0057588403736375
1101	10	0.9699587693259488
1101	11	1.0481036027480286
1101	12	1.073980444318181
1101	13	1.090480008172051
1101	14	1.28882517191105
1101	15	1.3234261362454802
1101	16	1.2956895687166892
1101	17	1.2799173750345645
1101	18	1.2800659328386175
1101	19	1.3766113984572286
1101	20	1.349339244627977
1101	21	1.3170433964411785
1101	22	1.314085920767262
1101	23	1.3955732007238595
1101	24	1.4276871413191898
1101	25	1.3553452955119099
1102	1	0.8472347901092192
1102	2	0.9134101866752751
1102	3	0.8779541630198153
1102	4	0.7327916966228827
1102	5	0.7834475738725786
1102	6	0.7528489015251267
1102	7	0.846816886577038
1102	8	0.8832581852636991
1102	9	0.9245859597013109
1102	10	0.9628978165895674
1102	11	0.9976894792591651
1102	12	1.011259152708412
1102	13	0.9773132185546558
1102	14	1.2001059131208578
1102	15	1.2553003638632845
1102	16	1.2345921283679502
1102	17	1.19995310973178
1102	18	1.183113245283046
1102	19	1.1481665999987385
1102	20	1.1232603267908416
1102	21	1.125256204524021
1102	22	1.2492051019714907
1102	23	1.2477771158899458
1102	24	1.2872407574212374
1102	25	1.2447847901006668
1103	1	0.7350851642346896
1103	2	0.7813345609692028
1103	3	0.8000377340619735
1103	4	0.6478080692252774
1103	5	0.7264213177930969
1103	6	0.733725424055585
1103	7	0.8169037990020698
1103	8	0.8072291513557843
1103	9	0.967803377962035
1103	10	0.9635558119856142
1103	11	0.9217102187572124
1103	12	0.9287594370906618
1103	13	1.0003621609566125
1103	14	1.160063190657965
1103	15	1.136559947110868
1103	16	1.1493597381416691
1103	17	1.1451850657866913
1103	18	1.1367758020835197
1103	19	1.115707218901349
1103	20	1.192200349489319
1103	21	1.1585187679238942
1103	22	1.213907594722997
1103	23	1.2802164903705702
1103	24	1.2637705298546535
1103	25	1.1644336670295843
1104	1	0.6690199019849032
1104	2	0.7162410210758914
1104	3	0.6453288294105448
1104	4	0.5741020974279749
1104	5	0.515263514918864
1104	6	0.5130706364751632
1104	7	0.5665567695785967
1104	8	0.6410913421861579
1104	9	0.7389250073032301
1104	10	0.8196451732696133
1104	11	0.7647541582747976
1104	12	0.8469308749886011
1104	13	0.7701595925025302
1104	14	0.9020982017082133
1104	15	0.9027848592168439
1104	16	0.913721213662086
1104	17	0.9098397934109812
1104	18	0.8501937845929817
1104	19	0.828552387186555
1104	20	0.8937789611384659
1104	21	0.9021316911350752
1104	22	0.8386582854676021
1104	23	0.9026705614752117
1104	24	0.794533121891476
1104	25	0.8221870441419981
1105	1	0.5117974059602624
1105	2	0.5556516239794931
1105	3	0.553650309860565
1105	4	0.48170560395768547
1105	5	0.41747992888574714
1105	6	0.477102787164263
1105	7	0.5864218164140667
1105	8	0.6116076312830359
1105	9	0.6628551746783629
1105	10	0.6868956344292911
1105	11	0.7375410653186592
1105	12	0.7517254738111347
1105	13	0.7494987742893855
1105	14	0.9451623994835856
1105	15	0.9661629010878983
1105	16	0.8940244737441958
1105	17	0.8626762925203124
1105	18	0.8859720979415613
1105	19	0.8645173127368011
1105	20	0.8716817501645977
1105	21	0.9126068263939686
1105	22	0.9442748805296759
1105	23	0.9538628138654204
1105	24	1.0113780256686056
1105	25	0.8704682478822221
1106	1	0.5024362006841336
1106	2	0.5243462401340402
1106	3	0.4961564268404608
1106	4	0.4527349980107189
1106	5	0.5225914856150808
1106	6	0.4474670371255769
1106	7	0.5227820710860271
1106	8	0.5643656713982494
1106	9	0.64308257170498
1106	10	0.6298534444671446
1106	11	0.6383502214254619
1106	12	0.7117264230211902
1106	13	0.6896596878198054
1106	14	0.7811219311314659
1106	15	0.7633649923321997
1106	16	0.7589069689955679
1106	17	0.8023214746519614
1106	18	0.737387054327904
1106	19	0.7581028652022042
1106	20	0.7796213163974244
1106	21	0.8044883164888716
1106	22	0.8366620098475437
1106	23	0.7337383061165994
1106	24	0.7532655074246506
1106	25	0.6875966583633186
1107	1	0.39818585509037163
1107	2	0.49399454962331646
1107	3	0.41753029318103785
1107	4	0.3661661795912181
1107	5	0.29985236044896624
1107	6	0.39544036254689274
1107	7	0.42485229178655004
1107	8	0.4463687290712173
1107	9	0.5217608386594179
1107	10	0.4704547325721862
1107	11	0.4663666948492562
1107	12	0.48284791946179695
1107	13	0.4852822346271637
1107	14	0.598776287385526
1107	15	0.5774959050222961
1107	16	0.6597424675267405
1107	17	0.5324456559282069
1107	18	0.49199778512685555
1107	19	0.6135846056173335
1107	20	0.6878153975539121
1107	21	0.6403832234379152
1107	22	0.6153959263337256
1107	23	0.6098012775574873
1107	24	0.70798405011017
1107	25	0.551459102019983
1108	1	0.3914556007173289
1108	2	0.4154948373454701
1108	3	0.40878487285118936
1108	4	0.30621893605830053
1108	5	0.31138479751113124
1108	6	0.28589748528094105
1108	7	0.36546171840851827
1108	8	0.45112458738305605
1108	9	0.4829389553346388
1108	10	0.469559072399818
1108	11	0.4857613896527013
1108	12	0.5637979913367914
1108	13	0.5924122450473703
1108	14	0.6520898689819745
1108	15	0.5738805484356817
1108	16	0.653053538497467
1108	17	0.5678013204997185
1108	18	0.6362222906113502
1108	19	0.6372419207586333
1108	20	0.571000037412432
1108	21	0.59593126192825
1108	22	0.7042431380950092
1108	23	0.6820467545430517
1108	24	0.6799165320525447
1108	25	0.6961896763311728
1109	1	0.2775638865211256
1109	2	0.3226148263589579
1109	3	0.27067584030794123
1109	4	0.22496563688615367
1109	5	0.10150079962260605
1109	6	0.16575472500576183
1109	7	0.17865314978788405
1109	8	0.21617224566821847
1109	9	0.3209871543621574
1109	10	0.33897783873474197
1109	11	0.2735311707378756
1109	12	0.2904946777877626
1109	13	0.1399957489720407
1109	14	0.3211224980627563
1109	15	0.3045386018490106
1109	16	0.28901118892898836
1109	17	0.3012999879560425
1109	18	0.37687592367045397
1109	19	0.3800363142044887
1109	20	0.37775942422775444
1109	21	0.3075580810327665
1109	22	0.3150413553618563
1109	23	0.2510611442981272
1109	24	0.2856375647846292
1109	25	0.27203630291423764
1110	1	-0.03095170212475651
1110	2	-0.01030038192801186
1110	3	-0.07864689827623295
1110	4	-0.05569523672543397
1110	5	-0.26323406189033705
1110	6	-0.19135171479434376
1110	7	-0.09911102768597146
1110	8	-0.11452099891470624
1110	9	-0.027723570545276743
1110	10	-0.10597734770181688
1110	11	-0.12560695215042017
1110	12	-0.14088392095381863
1110	13	-0.020485364589369226
1110	14	-0.025502828175937964
1110	15	-0.035864416744068706
1110	16	-0.1252826180995612
1110	17	-0.11396039604460384
1110	18	-0.10277639186558929
1110	19	-0.12232423772056933
1110	20	-0.10734082462408168
1110	21	-0.03178377665634713
1110	22	-0.11308699553947577
1110	23	-0.06613694581456286
1110	24	-0.18818999909746026
1110	25	-0.08432509874882044
1111	1	-0.14259143100901284
1111	2	-0.12232264243488089
1111	3	-0.1100775854352598
1111	4	-0.12588535021926361
1111	5	-0.1594061010011245
1111	6	-0.18624940411444157
1111	7	-0.03901785998410645
1111	8	-0.10635295702324044
1111	9	-0.0776502755973425
1111	10	-0.12821953083340532
1111	11	-0.14325308867548614
1111	12	-0.11721116400590859
1111	13	-0.005175221173068678
1111	14	-0.1652727047079523
1111	15	-0.19881967660110433
1111	16	-0.16004331387263449
1111	17	-0.20260345720432582
1111	18	-0.1656215203962148
1111	19	-0.25541180629290494
1111	20	-0.22378552849738043
1111	21	-0.1888478606206263
1111	22	-0.12027084495991147
1111	23	-0.1904907035029737
1111	24	-0.12396907905696253
1111	25	-0.18916898806588733
1112	1	-0.3076627449648335
1112	2	-0.48764478704240233
1112	3	-0.31443316540892496
1112	4	-0.35006102106316916
1112	5	-0.47605009371057605
1112	6	-0.432824328461912
1112	7	-0.43044557802473093
1112	8	-0.43726137603797355
1112	9	-0.5511631692303212
1112	10	-0.4550924215013268
1112	11	-0.448295754958437
1112	12	-0.39189852501891664
1112	13	-0.39065593086792916
1112	14	-0.5132923307620771
1112	15	-0.6197687779275327
1112	16	-0.5180084134200271
1112	17	-0.5572394093797186
1112	18	-0.5552996374112737
1112	19	-0.5308638683149027
1112	20	-0.5611560253186667
1112	21	-0.5649494007055651
1112	22	-0.5045033969137975
1112	23	-0.5675689678987581
1112	24	-0.598196529370469
1112	25	-0.6114572699903889
1113	1	-0.42803153405753136
1113	2	-0.48543556643659613
1113	3	-0.466060124436261
1113	4	-0.5744466068386984
1113	5	-0.6024056000708242
1113	6	-0.5434635160365815
1113	7	-0.5799002129252813
1113	8	-0.666925090986318
1113	9	-0.7232971912979309
1113	10	-0.6133640850054363
1113	11	-0.6244377888329211
1113	12	-0.5866776732373395
1113	13	-0.5703419594012118
1113	14	-0.781434505194575
1113	15	-0.7655246286549158
1113	16	-0.7067997901857908
1113	17	-0.7021719415323631
1113	18	-0.786964371405233
1113	19	-0.8507349063358447
1113	20	-0.7849906236741404
1113	21	-0.9168824248112628
1113	22	-0.822211949869029
1113	23	-0.745330405807259
1113	24	-0.8513019830214645
1113	25	-0.8313355116096162
1114	1	-0.6566284429822744
1114	2	-1.229184957873148
1114	3	-0.7932227667546954
1114	4	-0.6475529495645489
1114	5	-0.7220424478402805
1114	6	-0.6903010360017348
1114	7	-0.6275723829984798
1114	8	-0.7722224970113061
1114	9	-0.8223997630657109
1114	10	-0.7756269801446152
1114	11	-0.7877232627436968
1114	12	-0.842090578198949
1114	13	-0.7651270831210866
1114	14	-0.9924211466930473
1114	15	-1.03542606272935
1114	16	-0.9952359102414761
1114	17	-0.9801925942540279
1114	18	-0.9679320727009397
1114	19	-1.0392555206219214
1114	20	-0.9958914470498073
1114	21	-1.1040261055738299
1114	22	-1.0484209625869383
1114	23	-1.103540524615923
1114	24	-1.2266986169182346
1114	25	-1.2758247240217457
1115	1	-0.7837402049132614
1115	2	-1.1754950797314052
1115	3	-1.0573667672134945
1115	4	-0.8931138665353657
1115	5	-1.1156712817188605
1115	6	-1.0722108347839026
1115	7	-1.1610863570526035
1115	8	-1.2615153535177512
1115	9	-1.6400994557472544
1115	10	-1.3691747065513553
1115	11	-1.455703508333933
1115	12	-1.3172991560647063
1115	13	-1.6748300819775679
1115	14	-1.6416568704169379
1115	15	-1.6589542767904462
1115	16	-1.7334877176090089
1115	17	-1.6751770474786483
1115	18	-1.6131282033043561
1115	19	-1.5911845576678516
1115	20	-1.6937753958412642
1115	21	-1.6987836958301799
1115	22	-1.8693740279134587
1115	23	-1.8751444004741913
1115	24	-1.8038716745617478
1115	25	-1.71324050483814
1116	1	-0.6350025399937032
1116	2	-0.7165977789660154
1116	3	-0.6921678350338141
1116	4	-0.7457064368665698
1116	5	-0.8330159453969941
1116	6	-0.8001656872778753
1116	7	-0.9320773984246387
1116	8	-0.9257095412678638
1116	9	-1.0264040022320486
1116	10	-0.9049895639557235
1116	11	-0.9823247043529193
1116	12	-1.1738216234024108
1116	13	-1.0408352279909039
1116	14	-1.192998754819044
1116	15	-1.245156971555211
1116	16	-1.2989434993695852
1116	17	-1.4032348296126287
1116	18	-1.2451581966616772
1116	19	-1.2414807835478499
1116	20	-1.1701161950005812
1116	21	-1.1485317408260798
1116	22	-1.2491779554437343
1116	23	-1.4282761006767686
1116	24	-1.414899451914799
1116	25	-1.597962998737195
1201	1	0.7253451822863468
1201	2	0.8457360566779606
1201	3	0.7931190742650642
1201	4	0.7403230254296957
1201	5	0.7812496769115711
1201	6	0.734846181165409
1201	7	0.8373236501730403
1201	8	0.8248273271139226
1201	9	0.9397738523744295
1201	10	0.9745658746365251
1201	11	1.0137805954928325
1201	12	0.9911769814647116
1201	13	0.9729531390137822
1201	14	1.1539986109418763
1201	15	1.1429250816239787
1201	16	1.1489780038754915
1201	17	1.1836720825471758
1201	18	1.2094592639745585
1201	19	1.1978044258401042
1201	20	1.1759950480955297
1201	21	1.2450420222385432
1201	22	1.273502545204586
1201	23	1.366382950673703
1201	24	1.2962204506357864
1201	25	1.388064134412076
1202	1	0.6069251488393412
1202	2	0.7239470277643925
1202	3	0.595027957328881
1202	4	0.5773232532306096
1202	5	0.6978471744170491
1202	6	0.7467229120021912
1202	7	0.7599195001948615
1202	8	0.7765896258623926
1202	9	0.8342788055437609
1202	10	0.8329629536399992
1202	11	0.8261193020123656
1202	12	0.7367217872179808
1202	13	0.8221376431780811
1202	14	0.9620069564012539
1202	15	0.9744315059241941
1202	16	0.9712088293694165
1202	17	1.0501160788211905
1202	18	1.0238220259277497
1202	19	0.9660670851542648
1202	20	0.993632769754818
1202	21	1.0432730781057842
1202	22	1.0211895941937854
1202	23	1.0960216361280857
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2211	5	-0.3951149221583492
2211	6	-0.4142903179422248
2211	7	-0.5006553273269674
2211	8	-0.4673121402274063
2211	9	-0.5300178269934221
2211	10	-0.5689524333000335
2211	11	-0.6116217264928139
2211	12	-0.6179447928099293
2211	13	-0.6246192309368368
2211	14	-0.7139633203167506
2211	15	-0.6648926395752852
2211	16	-0.6667303852915083
2211	17	-0.6642686880225668
2211	18	-0.6423560084657751
2211	19	-0.6513668502152896
2211	20	-0.6979758025312961
2211	21	-0.6885879636427461
2211	22	-0.685877549811174
2211	23	-0.7500839517622921
2211	24	-0.725471994502314
2211	25	-0.6043266116918815
2212	1	-0.41515343320711295
2212	2	-0.4935810549859454
2212	3	-0.47252206734179936
2212	4	-0.4508595889481555
2212	5	-0.43929866263478345
2212	6	-0.491165975544682
2212	7	-0.5624881659229217
2212	8	-0.41853823894496145
2212	9	-0.5161014539748052
2212	10	-0.5757102334228108
2212	11	-0.5722323475682316
2212	12	-0.779573946923783
2212	13	-0.6161585901926969
2212	14	-0.7696858549246244
2212	15	-0.7408639137390765
2212	16	-0.7853157046499533
2212	17	-0.8068714722144605
2212	18	-0.8621599499365828
2212	19	-0.8372995345866627
2212	20	-0.8922406148523763
2212	21	-0.8229092782641061
2212	22	-0.8696404053544029
2212	23	-0.8021137256138857
2212	24	-0.8433293128496189
2212	25	-0.7750156873391774
2213	1	-0.7454226290345645
2213	2	-0.6426418192147878
2213	3	-0.5918009749326316
2213	4	-0.5618510371246686
2213	5	-0.6242736545974168
2213	6	-0.6066269476560819
2213	7	-0.6293460567539952
2213	8	-0.5944795434977976
2213	9	-0.7494832929426138
2213	10	-0.73636786334297
2213	11	-0.7139769798241815
2213	12	-0.7626092927365278
2213	13	-0.8740791797301455
2213	14	-1.059068969559661
2213	15	-0.9921493137934547
2213	16	-1.0338019386522248
2213	17	-1.0816029683511559
2213	18	-1.0814216984166407
2213	19	-1.085503760980977
2213	20	-1.0374772883652028
2213	21	-1.0411889887466614
2213	22	-1.0137329159380783
2213	23	-1.0642083877305026
2213	24	-1.0915875808878184
2213	25	-0.9320433942588977
2214	1	-0.7946741196420177
2214	2	-1.3071401931434927
2214	3	-0.902364426767928
2214	4	-0.8318267100807049
2214	5	-0.7939576745027281
2214	6	-0.8981418439345958
2214	7	-0.8387880941969712
2214	8	-0.9168698948788219
2214	9	-1.1527555195447334
2214	10	-1.1503511012765202
2214	11	-1.1095740787476416
2214	12	-1.1683428430111533
2214	13	-1.192101302628565
2214	14	-1.3865738470267672
2214	15	-1.3970171034983991
2214	16	-1.4074230353332382
2214	17	-1.263873341926498
2214	18	-1.2620717553015695
2214	19	-1.3713849647582634
2214	20	-1.3679642812664383
2214	21	-1.4173708188050895
2214	22	-1.3802886314755582
2214	23	-1.3595238712159485
2214	24	-1.3595192708260058
2214	25	-1.2839278539126795
2215	1	-1.0523928674830536
2215	2	-1.3087578080573117
2215	3	-1.244436902711488
2215	4	-1.0304284826921801
2215	5	-1.289557291484975
2215	6	-1.145617497817362
2215	7	-1.2307237077654776
2215	8	-1.3086933404547807
2215	9	-1.5784556155256837
2215	10	-1.4219559416716976
2215	11	-1.460191708384876
2215	12	-1.5797570927806532
2215	13	-1.6827527647459704
2215	14	-1.8581271293497323
2215	15	-1.9554869456416597
2215	16	-1.866962319787696
2215	17	-1.7602214134516032
2215	18	-1.7711601070018546
2215	19	-1.7488100476471473
2215	20	-1.8246004402719507
2215	21	-1.6761096637526833
2215	22	-1.739869812308747
2215	23	-1.8295967538392688
2215	24	-1.8701834630304575
2215	25	-1.7760870716517942
2216	1	-1.2070385711774065
2216	2	-1.2018342923824044
2216	3	-1.3823239895091177
2216	4	-1.2188442683114005
2216	5	-1.2959482452657838
2216	6	-1.1948098052916194
2216	7	-1.2770301117796308
2216	8	-1.4270594962482122
2216	9	-1.596124465004202
2216	10	-1.6514368709427956
2216	11	-1.6853563007243721
2216	12	-1.7565705839292889
2216	13	-1.5714556592115088
2216	14	-1.8238221970689565
2216	15	-1.9061607445292381
2216	16	-1.817040580629552
2216	17	-1.9575936302754613
2216	18	-1.9457046689606727
2216	19	-1.9221831282101434
2216	20	-1.9970243264666223
2216	21	-1.9478986445649014
2216	22	-2.069674817567865
2216	23	-2.0491032588436937
2216	24	-2.1114698756960664
2216	25	-2.0845091118481918
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47664.0
3	2.0
4	941.0
5	7517.0
6	8952.0
7	7287.0
8	5864.0
9	5195.0
10	4704.0
11	4311.0
12	3961.0
13	3908.0
14	3877.0
15	4140.0
16	4131.0
17	4397.0
18	4935.0
19	6043.0
20	7610.0
21	10608.0
22	13580.0
23	16636.0
24	20370.0
25	23941.0
26	26221.0
27	30032.0
28	35347.0
29	43135.0
30	55406.0
31	73678.0
32	95070.0
33	127522.0
34	171656.0
35	236703.0
36	341581.0
37	587906.0
38	1879457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.45676387196113	19.38890632142398	13.5194513819109	25.634878424703988
2	15.19840616576141	21.235705719648877	39.10456912399767	24.461318990592034
3	17.913262227441002	25.730450976075154	29.847834817424207	26.50845197905964
4	12.422153529371291	17.93484825703925	37.59927830033613	32.043719913253334
5	13.650215804457092	37.54252211839342	34.343500864162266	14.463761212987219
6	30.79158559040387	38.19020191717692	18.045211684566965	12.973000807852253
7	27.350268574091867	31.97620534712045	22.28363534062493	18.389890738162748
8	25.69049095208208	34.94015324621911	20.569185681016585	18.800170120682225
9	26.773142357978898	15.060306665484283	20.018597060961056	38.14795391557577
10	15.518436444366523	28.151559661830333	32.56001757308017	23.769986320722982
11	34.739693493229225	22.47208362426164	24.20205798859391	18.586164893915225
12	23.857465726074125	24.971529178101076	30.325725569562966	20.845279526261834
13	28.675999326209606	21.354721336449487	25.887465281573796	24.081814055767115
14	22.56648861951743	21.811850401657345	26.525975523635232	29.095685455189997
15	23.743398226615547	29.412927944629836	23.301415471180274	23.542258357574344
16	23.622754132432	27.443420586234918	25.877801768128183	23.0560235132049
17	22.42855111399253	27.43365560701673	26.56637599343934	23.571417285551405
18	22.81565162039013	26.662353339002326	28.414092706645388	22.107902333962155
19	24.68841179843282	25.775116264254315	26.676256609543223	22.860215327769637
20	24.791698520942276	26.13745247342079	26.41190891206181	22.65894009357512
21	25.671906209115182	25.45649562425748	25.57111964066467	23.30047852596267
22	24.69345641115549	26.17596078525309	26.1856696765079	22.944913127083524
23	23.508436451463872	25.865388315286015	26.586423139777292	24.039752093472817
24	23.70059684004274	26.44145878603026	26.772331554696834	23.08561281923017
25	23.80496581320734	25.9867279302146	26.830477850577083	23.377828406000972
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	210.0
1	210.0
2	576.5
3	943.0
4	943.0
5	943.0
6	2643.5
7	4344.0
8	4344.0
9	4344.0
10	6127.0
11	7910.0
12	7910.0
13	7910.0
14	12819.0
15	17728.0
16	17728.0
17	17728.0
18	30755.5
19	43783.0
20	43783.0
21	43783.0
22	70050.0
23	96317.0
24	96317.0
25	96317.0
26	140472.5
27	184628.0
28	184628.0
29	184628.0
30	229851.0
31	275074.0
32	275074.0
33	275074.0
34	307168.5
35	339263.0
36	339263.0
37	339263.0
38	363006.5
39	386750.0
40	386750.0
41	386750.0
42	415393.0
43	444036.0
44	444036.0
45	444036.0
46	466077.5
47	488119.0
48	488119.0
49	488119.0
50	479281.0
51	470443.0
52	470443.0
53	470443.0
54	419971.5
55	369500.0
56	369500.0
57	369500.0
58	328755.0
59	288010.0
60	288010.0
61	288010.0
62	251030.0
63	214050.0
64	214050.0
65	214050.0
66	175865.5
67	137681.0
68	137681.0
69	137681.0
70	104455.5
71	71230.0
72	71230.0
73	71230.0
74	56032.0
75	40834.0
76	40834.0
77	40834.0
78	32875.0
79	24916.0
80	24916.0
81	24916.0
82	18023.5
83	11131.0
84	11131.0
85	11131.0
86	8146.0
87	5161.0
88	5161.0
89	5161.0
90	3514.5
91	1868.0
92	1868.0
93	1868.0
94	1083.0
95	298.0
96	298.0
97	298.0
98	179.5
99	61.0
100	61.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.43732773945235415
2	0.0042300667025457865
3	0.0
4	0.005555147838283021
5	0.16871850384069673
6	0.03926826981098227
7	0.001146704829003376
8	3.822349430011253E-4
9	3.0578795440090023E-4
10	0.002650162271474469
11	0.001732798408271768
12	0.0024208213056737937
13	0.00558063016781643
14	0.010728060733564916
15	0.0038987964186114783
16	6.370582383352088E-4
17	0.007491804882822056
18	4.33199602067942E-4
19	9.683285222695173E-4
20	0.004281031361612604
21	0.003924278748144887
22	0.0012741164766704176
23	0.0017073160787383598
24	0.003083361873542411
25	0.006574441019619355
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3924288.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.02482576034289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.70387643139289	53.244347270613446
2	7.004557713043283	8.40894712507378
3	1.620497698766299	2.918102760404512
4	0.7283254762317849	1.7487043923052659
5	0.41483148891060573	1.2450093920881364
6	0.27773797858644456	1.000270426300871
7	0.189454938786768	0.7960399783078526
8	0.1397668983802989	0.6711586977872794
9	0.10917990381864161	0.5898154232920463
>10	0.6710128150292255	7.5141414637423685
>50	0.05981104774833853	2.534866668887692
>100	0.06401378874079104	8.298100373248033
>500	0.010849558424523956	4.504966051665867
>1k	0.005999167564792179	6.106921287718165
>5k	4.2547287693561544E-5	0.15749861401860943
>10k+	4.2547287693561544E-5	0.26111007454617524
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10224	0.260531337149567	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6167	0.1571495262325293	No Hit
GTATCAACGCAGAGTACATGGGGAA	4157	0.10593004387037853	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3925	0.10001814341862778	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5482329533408354E-5
2	0.0	0.0	0.0	0.0	2.5482329533408354E-5
3	0.0	0.0	0.0	0.0	2.5482329533408354E-5
4	0.0	0.0	0.0	2.5482329533408354E-5	2.5482329533408354E-5
5	0.0	0.0	0.0	5.096465906681671E-5	2.5482329533408354E-5
6	0.0	0.0	0.0	5.096465906681671E-5	7.644698860022506E-5
7	0.0	0.0	0.0	7.644698860022506E-5	7.644698860022506E-5
8	0.0	0.0	0.0	7.644698860022506E-5	7.644698860022506E-5
9	0.0	0.0	0.0	1.0192931813363342E-4	1.0192931813363342E-4
10	2.5482329533408354E-5	0.0	0.0	1.0192931813363342E-4	1.0192931813363342E-4
11	2.5482329533408354E-5	0.0	0.0	1.0192931813363342E-4	1.0192931813363342E-4
12	2.5482329533408354E-5	0.0	0.0	1.2741164766704176E-4	3.5675261346771695E-4
13	2.5482329533408354E-5	0.0	0.0	1.2741164766704176E-4	4.0771727253453366E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1790	0.0	16.365217	1
GTATCAA	6210	0.0	13.521583	1
CGAGCCG	480	0.0	11.866167	15
CGAACGT	265	0.0	11.845994	4
GCGTTAT	225	0.0	11.026237	1
CGAACGA	310	0.0	11.024053	16
TCGAACG	285	0.0	11.014697	3
TAAACCG	95	1.341548E-5	11.014696	5
AACCGCG	275	0.0	10.7012615	7
ATTCGAA	305	0.0	10.636911	1
CGTCGTA	515	0.0	10.50701	10
CAAGACG	470	0.0	10.119885	4
TCCAACG	480	0.0	10.086114	18
CCAACGA	330	0.0	10.068648	19
CCGATAA	340	0.0	10.051598	9
CGCATCG	445	0.0	10.026628	13
AGAACCG	380	0.0	10.01336	5
GTATTAG	745	0.0	9.990214	1
AGGCCCG	590	0.0	9.97588	10
CGGTCCA	545	0.0	9.928642	10
>>END_MODULE