FastQCFastQC Report
Thu 2 Feb 2017
SRR4050215_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050215_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295082
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN95220.2216954181549968No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN71120.1655847315604219No Hit
GATATACACTGTTCTACAAATCCCG57840.1346656478269798No Hit
TTGTAGAACAGTGTATATCAATGAG57800.13457251805669834No Hit
GTATCAACGCAGAGTACTTTTTTTT49680.11566717468956356No Hit
GTGTATATCAATGAGTTACAATGAA45470.1058652663674407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.015.9781741
GTATCAA55800.013.4516711
GTATACG604.0898795E-412.6684091
TCCAACG10400.012.24020318
CCAACGA8000.011.39969719
CGCGCCA1251.8366336E-811.39929710
ACCGTCG5250.011.217968
CCGTCGT5000.011.2090459
TACCGTC5400.011.082787
CGTCGTA5200.010.96086210
ATACCGT5900.010.7881036
GCTCGTA3300.010.6506089
ACTGTTC24950.010.3557118
CCAACGT3700.010.26999819
AGCCCCG5200.010.23061816
TGTAGAA30100.010.1955162
CGCATCG3450.010.18801613
ACGCATC2800.010.17794310
CGGTCCA5850.010.06775510
GAACAGT16500.010.018156
Produced by FastQC (version 0.11.5)