##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050215_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4295082
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.968010156732745	31.0	30.0	34.0	26.0	34.0
2	30.103994289282486	31.0	31.0	34.0	26.0	34.0
3	30.224848326527876	31.0	31.0	34.0	26.0	34.0
4	33.62755495704157	37.0	35.0	37.0	28.0	37.0
5	33.695699639727486	37.0	35.0	37.0	28.0	37.0
6	33.79322397104409	37.0	35.0	37.0	30.0	37.0
7	33.726967494450626	37.0	35.0	37.0	29.0	37.0
8	33.65453511714095	37.0	35.0	37.0	29.0	37.0
9	35.16400199111449	39.0	35.0	39.0	29.0	39.0
10	35.043539564553136	39.0	35.0	39.0	28.0	39.0
11	35.087658629101846	39.0	35.0	39.0	29.0	39.0
12	34.97777853833757	39.0	35.0	39.0	28.0	39.0
13	34.99226370998272	39.0	35.0	39.0	28.0	39.0
14	35.83373402416997	40.0	36.0	41.0	27.0	41.0
15	35.96359580562141	40.0	36.0	41.0	27.0	41.0
16	35.98191280166479	40.0	36.0	41.0	27.0	41.0
17	35.97582490858149	40.0	36.0	41.0	27.0	41.0
18	35.81036497091324	40.0	36.0	41.0	27.0	41.0
19	35.95421577515866	40.0	36.0	41.0	27.0	41.0
20	35.91683860750505	40.0	36.0	41.0	27.0	41.0
21	35.87963815359055	40.0	36.0	41.0	27.0	41.0
22	35.802286661814605	40.0	36.0	41.0	27.0	41.0
23	35.698696788559566	40.0	36.0	41.0	27.0	41.0
24	35.629412663134254	40.0	36.0	41.0	27.0	41.0
25	35.34980263473433	39.0	36.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.1550868464452009
1101	2	1.1746113673104972
1101	3	1.097354116507514
1101	4	1.1726083905883726
1101	5	1.1509228179951734
1101	6	1.0689190302076668
1101	7	1.1615593693676516
1101	8	1.151706072629267
1101	9	1.335227139732126
1101	10	1.3021271434210107
1101	11	1.2735404244278854
1101	12	1.3700890532554553
1101	13	1.4426438732501339
1101	14	1.8634057909142925
1101	15	1.7479791595525853
1101	16	1.734556653980178
1101	17	1.7194105090832181
1101	18	1.8997271213183566
1101	19	1.6828283629568475
1101	20	1.741836313970488
1101	21	1.6727321842513305
1101	22	1.7788639649329596
1101	23	1.7002349088581923
1101	24	1.7305167233021237
1101	25	1.785939866247169
1102	1	0.9928380172075251
1102	2	0.93082855780278
1102	3	0.9300910450065238
1102	4	0.9862983437578805
1102	5	0.961973205030688
1102	6	1.0054741727107697
1102	7	1.0021577053563249
1102	8	0.9982929182495681
1102	9	1.1887706470302817
1102	10	1.2328331412645568
1102	11	1.154575523158158
1102	12	1.1688698511440592
1102	13	1.2474324923138411
1102	14	1.6700737450171914
1102	15	1.5717764527179057
1102	16	1.485285181957778
1102	17	1.4033771001130049
1102	18	1.5751242149310585
1102	19	1.4845306755141792
1102	20	1.4647153432435687
1102	21	1.483816044628874
1102	22	1.51875002682646
1102	23	1.4992432611007445
1102	24	1.5586980586953558
1102	25	1.4721366186892695
1103	1	0.7320847760518276
1103	2	0.7582477309468629
1103	3	0.6958352006407722
1103	4	0.7658391453408626
1103	5	0.7277381765496642
1103	6	0.7294592146608991
1103	7	0.7208206153680266
1103	8	0.7493006519629759
1103	9	0.8388795018627491
1103	10	0.9011540585348854
1103	11	0.8618154103115714
1103	12	0.9024822397790757
1103	13	0.9193839447728109
1103	14	1.286764533620861
1103	15	1.1556546724454861
1103	16	1.0495321856948436
1103	17	1.0108654309729275
1103	18	1.1281526812656537
1103	19	1.0400013349124322
1103	20	0.9585048628129513
1103	21	1.015425577814291
1103	22	1.0563220865215186
1103	23	0.9806056974898496
1103	24	1.071942471467004
1103	25	1.1352688705761693
1104	1	0.7590221619809725
1104	2	0.7777380779059939
1104	3	0.7448108141798748
1104	4	0.8614366111759537
1104	5	0.736230934018387
1104	6	0.7525793728918515
1104	7	0.7938375263223136
1104	8	0.805446853225888
1104	9	0.8366406259810333
1104	10	0.9213976496835699
1104	11	0.8834748260081469
1104	12	0.9467535087394339
1104	13	0.9773335951816264
1104	14	1.3119464594776602
1104	15	1.1668487046877374
1104	16	1.128591767561261
1104	17	1.1146520439388325
1104	18	1.290074571824718
1104	19	1.1658809743636027
1104	20	1.1613591898838251
1104	21	1.1006626646676239
1104	22	1.1635543256505656
1104	23	1.0659887010365026
1104	24	1.11645478070443
1104	25	1.1778347515359187
1105	1	0.5999633295554858
1105	2	0.6611971745570422
1105	3	0.5780516527704869
1105	4	0.7160984646820054
1105	5	0.5945909203290967
1105	6	0.6025488480814545
1105	7	0.6808205232920841
1105	8	0.6910879381165955
1105	9	0.7430395348761607
1105	10	0.833534949930467
1105	11	0.7499858278173832
1105	12	0.805195215623371
1105	13	0.7727647223290504
1105	14	1.1363691511457574
1105	15	1.024016415392886
1105	16	1.0568877980584617
1105	17	0.9324441400012233
1105	18	1.0718566119040105
1105	19	0.9550470464334708
1105	20	0.9336830716177502
1105	21	1.0572853947003509
1105	22	1.0305571446884016
1105	23	0.9876122583194018
1105	24	1.0270378705157768
1105	25	1.0081703761015604
1106	1	0.5567747354971075
1106	2	0.47479253744982586
1106	3	0.47100044228773186
1106	4	0.5894436489299082
1106	5	0.5110944486270554
1106	6	0.44210979419341356
1106	7	0.4164146767752612
1106	8	0.5203441167484826
1106	9	0.631175988651691
1106	10	0.5794985059806592
1106	11	0.6227418630017141
1106	12	0.66199347302598
1106	13	0.7378413953932608
1106	14	0.9949527005365155
1106	15	0.8221513657749995
1106	16	0.7797507346554298
1106	17	0.7640228005574343
1106	18	0.9484289231582324
1106	19	0.8703278245985686
1106	20	0.870023787429929
1106	21	0.8971294946737487
1106	22	0.8950389042041351
1106	23	0.8681671099284713
1106	24	0.8517679685701083
1106	25	0.9228747057761453
1107	1	0.3048689240912523
1107	2	0.33392939961471413
1107	3	0.36421197703168673
1107	4	0.45106927083458714
1107	5	0.2581498169968768
1107	6	0.247776375483717
1107	7	0.2709905262377035
1107	8	0.3034873129971132
1107	9	0.3411835920647803
1107	10	0.318449283908933
1107	11	0.33356664687706683
1107	12	0.24889146940112283
1107	13	0.2451825743648186
1107	14	0.5869777485351335
1107	15	0.4990460451996981
1107	16	0.285017755047015
1107	17	0.22399943768915165
1107	18	0.34600560372861366
1107	19	0.350979680289889
1107	20	0.4073975893936108
1107	21	0.33022172977488395
1107	22	0.3757233435989278
1107	23	0.3895960471885118
1107	24	0.3637154480630187
1107	25	0.3374445287826475
1108	1	0.2998498226781443
1108	2	0.3643403504714158
1108	3	0.30963262580383244
1108	4	0.39094512780226154
1108	5	0.34957187594999795
1108	6	0.40814832892987596
1108	7	0.3219336002278439
1108	8	0.33261758088155347
1108	9	0.3507225448200515
1108	10	0.4331867106773828
1108	11	0.43667486921642507
1108	12	0.3587829169930856
1108	13	0.4123193089925792
1108	14	0.7883905093852732
1108	15	0.5919512594920846
1108	16	0.6302182938796719
1108	17	0.548090130096071
1108	18	0.6885480197076177
1108	19	0.5682887100350626
1108	20	0.5733069341039538
1108	21	0.4844614993069385
1108	22	0.532967320074647
1108	23	0.6235347783216625
1108	24	0.6219116609934687
1108	25	0.5457032018149093
1109	1	0.14761542701955577
1109	2	0.22529051797204147
1109	3	0.17897589261872682
1109	4	0.25410959478323747
1109	5	0.19341009011272803
1109	6	0.22478757480860878
1109	7	0.251566685994689
1109	8	0.24600916390418348
1109	9	0.1937588603589191
1109	10	0.20704208763173426
1109	11	0.19664023541502473
1109	12	0.2806351327628036
1109	13	0.3086064669944122
1109	14	0.5806858348140551
1109	15	0.37469346050983887
1109	16	0.31754084701719165
1109	17	0.357367036124252
1109	18	0.41605234539005664
1109	19	0.3560271008386877
1109	20	0.41649837725930894
1109	21	0.4536806020307367
1109	22	0.36880063038019273
1109	23	0.30648041218115907
1109	24	0.3238659855422341
1109	25	0.3011352811608887
1110	1	-0.0603738903418467
1110	2	-0.018563357021193383
1110	3	-0.06574435894616215
1110	4	0.003081722578706092
1110	5	-0.08694209020985255
1110	6	-0.06336122215227391
1110	7	-0.08046165405653483
1110	8	-0.0424766129551557
1110	9	-0.0094885610558606
1110	10	-0.010705720787974826
1110	11	-0.022551199900533447
1110	12	-0.05505002378628632
1110	13	-0.06414036096828823
1110	14	0.18326685864500547
1110	15	-0.03672047457354921
1110	16	-0.15184862140474564
1110	17	-0.21140550854082107
1110	18	-0.00825119608077074
1110	19	-0.02980273157832869
1110	20	-0.239711765576736
1110	21	-0.11470026091841135
1110	22	-0.1542676480108085
1110	23	-0.027420609097596582
1110	24	-0.1556090365222289
1110	25	-0.19815017112876632
1111	1	-0.4671839302930785
1111	2	-0.4337445567581497
1111	3	-0.5823449337595328
1111	4	-0.3666006144972016
1111	5	-0.45053183148787923
1111	6	-0.5256649858127176
1111	7	-0.5167376603821339
1111	8	-0.48195414663445035
1111	9	-0.6473151986867762
1111	10	-0.5998369620166031
1111	11	-0.5013954207209537
1111	12	-0.559015379540952
1111	13	-0.637013021830029
1111	14	-0.3584268130373758
1111	15	-0.5855378550185009
1111	16	-0.5831984581932872
1111	17	-0.5822183645008678
1111	18	-0.5091155379339156
1111	19	-0.5752142934582807
1111	20	-0.6716982199463502
1111	21	-0.6061391301251717
1111	22	-0.70193752740321
1111	23	-0.6494133565847235
1111	24	-0.7646592149049454
1111	25	-0.7692314852485964
1112	1	-0.6492691901496421
1112	2	-0.6417241540996521
1112	3	-0.7038102267463806
1112	4	-0.6176556629592653
1112	5	-0.6824135538486615
1112	6	-0.7003696614690824
1112	7	-0.7059388014140993
1112	8	-0.7425555780502933
1112	9	-0.8053555907013461
1112	10	-0.7458320078217184
1112	11	-0.7917050575340099
1112	12	-0.6825958549518418
1112	13	-0.798056520883101
1112	14	-0.7570132790570199
1112	15	-0.814712751199032
1112	16	-0.9910897367408396
1112	17	-0.9907749622962783
1112	18	-0.870451469755757
1112	19	-0.8832711500083334
1112	20	-0.9188953384029546
1112	21	-0.8999379155829459
1112	22	-0.9117519621008796
1112	23	-1.061346304066305
1112	24	-1.055722735773152
1112	25	-0.9652772318356071
1113	1	-0.9018529055321451
1113	2	-0.9836638662278929
1113	3	-0.9322587142707306
1113	4	-0.8442996262068618
1113	5	-1.0055879394178433
1113	6	-1.0782972247153495
1113	7	-1.205528642870739
1113	8	-1.2434646463760402
1113	9	-1.1654277442310246
1113	10	-1.332837541017355
1113	11	-1.2671626319142675
1113	12	-1.182206295506063
1113	13	-1.2881296514707614
1113	14	-1.2734288281007835
1113	15	-1.3063708388199728
1113	16	-1.345159194081532
1113	17	-1.4158423959065303
1113	18	-1.303793042728124
1113	19	-1.2887447256087228
1113	20	-1.5494158423944526
1113	21	-1.3711858901554663
1113	22	-1.4343041827088712
1113	23	-1.828568918893005
1113	24	-1.6559601832499666
1113	25	-1.6937191839757944
1114	1	-2.471861756022175
1114	2	-2.7225819628420567
1114	3	-2.289796377998652
1114	4	-5.424199925574001
1114	5	-2.9983135680115005
1114	6	-2.524352400286208
1114	7	-2.251152698540217
1114	8	-2.5135319002712038
1114	9	-3.106172752261223
1114	10	-2.864363671644689
1114	11	-2.561413672746639
1114	12	-2.758327845415785
1114	13	-2.7409078677016936
1114	14	-2.706113754269367
1114	15	-3.4048668156268604
1114	16	-3.3107499328524455
1114	17	-3.0228712973891376
1114	18	-2.9654201120162043
1114	19	-3.0318281392006625
1114	20	-3.2220027106840377
1114	21	-3.1711710851343753
1114	22	-3.30860527152295
1114	23	-3.4329634336417243
1114	24	-3.452152788588222
1114	25	-3.3399287046920563
1115	1	-2.7921335380014014
1115	2	-2.2814784767125786
1115	3	-1.8038122649465613
1115	4	-1.7956370320579218
1115	5	-1.700163686308862
1115	6	-1.7307462341882243
1115	7	-1.8100448295726856
1115	8	-1.7447834567493956
1115	9	-2.128631437118713
1115	10	-1.9465602383156337
1115	11	-1.9550904317562328
1115	12	-2.4901224446599386
1115	13	-2.654896624882035
1115	14	-2.8304303625283254
1115	15	-2.624084669322208
1115	16	-2.4814062513462787
1115	17	-2.4473392833959764
1115	18	-2.3395430399222263
1115	19	-2.3613331913254783
1115	20	-2.417754977264174
1115	21	-2.3711979336698334
1115	22	-2.486211456321044
1115	23	-2.4779362645199257
1115	24	-2.780273265647722
1115	25	-2.9653312067337723
1116	1	-1.5572476232088555
1116	2	-1.5889394461372213
1116	3	-1.654780827586194
1116	4	-1.508912082066125
1116	5	-1.5532515643235385
1116	6	-1.7220669597473943
1116	7	-1.8097035258127718
1116	8	-1.8503154797015675
1116	9	-2.1125846361208858
1116	10	-2.4741046660066743
1116	11	-2.2267233667637356
1116	12	-2.279749561737802
1116	13	-2.1398408585129545
1116	14	-2.9795270151113016
1116	15	-2.9234426832094655
1116	16	-2.852047875510948
1116	17	-2.5727972831245722
1116	18	-2.5273629707201906
1116	19	-2.4598069398846363
1116	20	-2.483274932171689
1116	21	-2.665735670917556
1116	22	-2.487701703076006
1116	23	-2.8825241877153402
1116	24	-2.897086463888691
1116	25	-2.8338663756209286
1201	1	1.0921575286108087
1201	2	1.0885790036322938
1201	3	1.0163354182571958
1201	4	1.1719493120073068
1201	5	1.105951202336641
1201	6	1.051533228803919
1201	7	1.0533241297482192
1201	8	1.07221090895046
1201	9	1.225459788225045
1201	10	1.2800612056767875
1201	11	1.2304772466667444
1201	12	1.311566383887083
1201	13	1.245048629924824
1201	14	1.6487532544046601
1201	15	1.4979537115222996
1201	16	1.5093748492471164
1201	17	1.4492945359986606
1201	18	1.613992662473784
1201	19	1.536616114728723
1201	20	1.5130681450780514
1201	21	1.4805752283318654
1201	22	1.5531014430226975
1201	23	1.6117361932141563
1201	24	1.5356580024579003
1201	25	1.6246243689496183
1202	1	0.9763283272997185
1202	2	0.9821723128144164
1202	3	0.9370170537060929
1202	4	1.0027903674709577
1202	5	0.9737051069892715
1202	6	0.9258097294608945
1202	7	0.9202915265519493
1202	8	0.9086323913885792
1202	9	1.10140368552171
1202	10	1.1107931752845985
1202	11	1.053005514666907
1202	12	1.1192815308389683
1202	13	1.2380004650629317
1202	14	1.5662810080257614
1202	15	1.4419107066205825
1202	16	1.4420074787833457
1202	17	1.3722129001748158
1202	18	1.589522220991256
1202	19	1.4265219470374007
1202	20	1.409659909248255
1202	21	1.3600886702636217
1202	22	1.4262720286954007
1202	23	1.537113617386595
1202	24	1.4919743898718067
1202	25	1.440436123554015
1203	1	0.7327521868851079
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2211	10	-0.036870350313321865
2211	11	-0.05871561674456416
2211	12	0.02966553456568022
2211	13	-0.011366553887228292
2211	14	0.10860676125457758
2211	15	0.021248965082449445
2211	16	-0.04563795017727301
2211	17	-0.13330952629935666
2211	18	-0.024562211639711506
2211	19	0.01402614350447351
2211	20	-0.0022610953281585466
2211	21	-0.014949855071577645
2211	22	-0.0975012797294994
2211	23	-0.09132473892436366
2211	24	-0.02803580775814396
2211	25	-0.08636308364092571
2212	1	-0.37329250679976056
2212	2	-0.41988855959466775
2212	3	-0.4662793830046894
2212	4	-0.33160777918259043
2212	5	-0.4259570170790141
2212	6	-0.42209865874247754
2212	7	-0.520323672928896
2212	8	-0.45458772087334864
2212	9	-0.5299998271902169
2212	10	-0.6188207623373714
2212	11	-0.636967152075556
2212	12	-0.5760744822671455
2212	13	-0.5600542510468927
2212	14	-0.6727345034341354
2212	15	-0.6449710375051296
2212	16	-0.6395591819413085
2212	17	-0.8296546228902528
2212	18	-0.5228654480695312
2212	19	-0.5964967777880048
2212	20	-0.7102838241041667
2212	21	-0.7222473641120928
2212	22	-0.9076877190825385
2212	23	-0.9684589610210637
2212	24	-0.8421838772689085
2212	25	-0.856120035716252
2213	1	-0.8740841195750804
2213	2	-0.5657207206928234
2213	3	-0.6071308505801944
2213	4	-0.47601107214067895
2213	5	-0.5220724971919211
2213	6	-0.6556471798379917
2213	7	-0.6648742426824015
2213	8	-0.590070005174212
2213	9	-0.6324790809877925
2213	10	-0.7119919338487506
2213	11	-0.786306577884865
2213	12	-0.7605347977793571
2213	13	-0.8677593764681859
2213	14	-0.6110975596164607
2213	15	-0.809817199339669
2213	16	-0.8078272634722339
2213	17	-0.8478657762752562
2213	18	-0.7974957927538711
2213	19	-0.8833395786905456
2213	20	-0.831099699787444
2213	21	-0.8045067412067723
2213	22	-1.0311200236811615
2213	23	-0.8796957084696828
2213	24	-0.9115772101616741
2213	25	-0.9506609758331308
2214	1	-1.079746356114903
2214	2	-0.9978830704291326
2214	3	-0.9427127361738066
2214	4	-0.7640629792230698
2214	5	-0.8051426084956148
2214	6	-0.9845714421123901
2214	7	-0.9545535811695771
2214	8	-1.0128273601620918
2214	9	-1.0074570540655827
2214	10	-1.1981367956562536
2214	11	-1.1501390767807393
2214	12	-1.0503156048517255
2214	13	-1.2862272899044243
2214	14	-1.074093332377302
2214	15	-1.2912303368121556
2214	16	-1.1633267197579755
2214	17	-1.2457856331652124
2214	18	-1.1026866123335282
2214	19	-1.2159945372532093
2214	20	-1.3360473881668042
2214	21	-1.3505915903214287
2214	22	-1.3863056473566715
2214	23	-1.4045635654932482
2214	24	-1.41648641173677
2214	25	-1.4002300498116682
2215	1	-1.6428780181475702
2215	2	-1.7698152913022085
2215	3	-1.649469099036633
2215	4	-1.7808311543628008
2215	5	-1.7180699334394376
2215	6	-1.4892758756616544
2215	7	-1.626993434417713
2215	8	-1.7628267976012815
2215	9	-2.141833667677517
2215	10	-1.8058004715996248
2215	11	-1.8846615286577872
2215	12	-1.9873628746615921
2215	13	-1.9577542944736166
2215	14	-2.731844385266122
2215	15	-2.3788651976406143
2215	16	-2.033105180309562
2215	17	-2.087609021773517
2215	18	-2.0595122826884946
2215	19	-2.15388411061609
2215	20	-2.4676739044932177
2215	21	-2.2250273043221114
2215	22	-2.5005672240331123
2215	23	-2.0884482684296373
2215	24	-2.192689256764318
2215	25	-2.154333968445272
2216	1	-1.717572097337925
2216	2	-1.6843745890314281
2216	3	-1.729734212887994
2216	4	-1.5156876042869598
2216	5	-2.074875964606175
2216	6	-1.7748540839036338
2216	7	-1.8894410814135796
2216	8	-2.385528838785568
2216	9	-2.2911555636937493
2216	10	-2.3325274823524467
2216	11	-2.283996469903549
2216	12	-2.421854542960297
2216	13	-2.3591123565290886
2216	14	-2.267914500432738
2216	15	-2.2522849669994756
2216	16	-2.330541317157092
2216	17	-2.3720888216889335
2216	18	-2.6372088741124955
2216	19	-2.4694051914849666
2216	20	-2.6328997301840857
2216	21	-2.8347620912899956
2216	22	-2.9648031258962746
2216	23	-2.7500371396386925
2216	24	-2.870623689553298
2216	25	-2.9290177261760064
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	150136.0
3	9.0
4	1125.0
5	6600.0
6	8167.0
7	7401.0
8	6532.0
9	6203.0
10	5926.0
11	5725.0
12	5618.0
13	5465.0
14	5463.0
15	5614.0
16	5998.0
17	6292.0
18	6986.0
19	8634.0
20	10978.0
21	14006.0
22	17746.0
23	21326.0
24	25326.0
25	28735.0
26	33230.0
27	37718.0
28	43286.0
29	51991.0
30	65538.0
31	84654.0
32	108594.0
33	143533.0
34	194807.0
35	271004.0
36	394788.0
37	680193.0
38	1819735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02602673939798	18.69664686437225	13.053693013565715	25.223633382664058
2	14.980188927497965	20.852486536226884	40.48666651201165	23.680658024263497
3	18.39375433785118	25.494848940849636	29.846290689102663	26.265106032196524
4	12.03327981042956	17.45556708500768	38.61478319474557	31.89636990981719
5	13.737250060880015	37.31392923011801	34.5658557690933	14.382964939908671
6	30.664941720963856	37.91027625697721	18.49542478389734	12.929357238161588
7	27.15528466280361	31.719626470533775	22.47993131160748	18.64515755505514
8	25.286321515922506	35.213063803705424	21.049796727720622	18.450817952651448
9	26.664365147499957	15.178354845037683	19.97535827645745	38.181921731004905
10	15.202004731049026	28.36665372147654	32.974961721064986	23.456379826409453
11	34.857670959738066	22.257960706314115	24.489679682605384	18.394688651342427
12	23.51506597141571	24.569122616383712	31.12653578404062	20.789275628159963
13	28.736497495907763	21.268166621978636	26.08591914528985	23.90941673682375
14	22.320511002705707	21.787032989954078	26.96324385005056	28.92921215728965
15	23.835601926374398	29.38937362832074	23.40601612311996	23.3690083221849
16	23.640532882795036	27.304711525479664	26.265170039453356	22.789585552271944
17	22.4011965268656	27.15437184214696	26.961865276812862	23.482566354174576
18	22.45447503465862	27.00499107606381	28.64997193325388	21.890561956023685
19	24.729125069697748	25.7594212959762	26.39146475315336	23.11998888117269
20	24.395852748864133	26.71589538958884	26.379541827850733	22.508710033696296
21	25.670085985324885	25.680562853945887	25.359998799964938	23.28935236076429
22	24.330731952451355	26.336918826228445	26.304128113945737	23.028221107374463
23	23.767529001213468	25.914313781515403	26.209629122466442	24.108528094804687
24	23.745838448226113	26.61656200914317	26.475122575763503	23.162476966867214
25	23.990697223112193	26.025415996454388	26.695186869987765	23.288699910445658
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15843.0
1	15843.0
2	14164.5
3	12486.0
4	12486.0
5	12486.0
6	8295.0
7	4104.0
8	4104.0
9	4104.0
10	5904.0
11	7704.0
12	7704.0
13	7704.0
14	12973.5
15	18243.0
16	18243.0
17	18243.0
18	32182.5
19	46122.0
20	46122.0
21	46122.0
22	75195.0
23	104268.0
24	104268.0
25	104268.0
26	153113.0
27	201958.0
28	201958.0
29	201958.0
30	258051.5
31	314145.0
32	314145.0
33	314145.0
34	348165.0
35	382185.0
36	382185.0
37	382185.0
38	402147.5
39	422110.0
40	422110.0
41	422110.0
42	454019.0
43	485928.0
44	485928.0
45	485928.0
46	505712.5
47	525497.0
48	525497.0
49	525497.0
50	513844.0
51	502191.0
52	502191.0
53	502191.0
54	451139.0
55	400087.0
56	400087.0
57	400087.0
58	355520.5
59	310954.0
60	310954.0
61	310954.0
62	270508.5
63	230063.0
64	230063.0
65	230063.0
66	188420.5
67	146778.0
68	146778.0
69	146778.0
70	111674.0
71	76570.0
72	76570.0
73	76570.0
74	59847.5
75	43125.0
76	43125.0
77	43125.0
78	34367.0
79	25609.0
80	25609.0
81	25609.0
82	18482.5
83	11356.0
84	11356.0
85	11356.0
86	8413.5
87	5471.0
88	5471.0
89	5471.0
90	3712.0
91	1953.0
92	1953.0
93	1953.0
94	1106.5
95	260.0
96	260.0
97	260.0
98	166.0
99	72.0
100	72.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22630534178392867
2	0.6407095370938204
3	0.6383114455090729
4	0.6460877813275743
5	0.6633866361573539
6	0.6764480864393275
7	0.6719080101381069
8	0.6667393078874863
9	0.6597080102312366
10	0.6606393079340511
11	0.665761445299531
12	0.6661805292657975
13	0.6627580102079541
14	0.6560293843051193
15	0.6615473231942952
16	0.6700221322899074
17	0.6689977048168114
18	0.6627812926505245
19	0.6622690789139766
20	0.6690908345870928
21	0.664480910958161
22	0.6665763307894936
23	0.6704179338136036
24	0.673910300199158
25	0.6611980865557399
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4295082.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.237925488164414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.67750609320231	52.530714984253635
2	6.948185637377947	8.23192206129858
3	1.629616720248392	2.8960534154502326
4	0.7255417797892633	1.7191835955882626
5	0.42016946339753014	1.2444983682572457
6	0.2820344639258199	1.0024281935479267
7	0.19871256807271753	0.8239924210737407
8	0.14593696096086145	0.6916002255494935
9	0.12492619601697345	0.6660331822055965
>10	0.7028874811194925	7.751380714021584
>50	0.06353007322546546	2.6575114696333286
>100	0.06371527817679919	8.1706460349486
>500	0.010922242558996237	4.419767349371692
>1k	0.006156172948132628	6.530834692666257
>5k	1.588689793066482E-4	0.6634332921338383
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	9522	0.2216954181549968	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	7112	0.1655847315604219	No Hit
GATATACACTGTTCTACAAATCCCG	5784	0.1346656478269798	No Hit
TTGTAGAACAGTGTATATCAATGAG	5780	0.13457251805669834	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4968	0.11566717468956356	No Hit
GTGTATATCAATGAGTTACAATGAA	4547	0.1058652663674407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.98473277110891E-5
2	0.0	0.0	0.0	0.0	6.98473277110891E-5
3	0.0	0.0	0.0	2.3282442570363033E-5	6.98473277110891E-5
4	0.0	0.0	0.0	2.3282442570363033E-5	6.98473277110891E-5
5	0.0	0.0	0.0	2.3282442570363033E-5	6.98473277110891E-5
6	0.0	0.0	0.0	2.3282442570363033E-5	6.98473277110891E-5
7	0.0	0.0	0.0	2.3282442570363033E-5	6.98473277110891E-5
8	0.0	0.0	0.0	4.6564885140726065E-5	6.98473277110891E-5
9	0.0	0.0	0.0	9.312977028145213E-5	6.98473277110891E-5
10	0.0	0.0	0.0	9.312977028145213E-5	6.98473277110891E-5
11	0.0	0.0	0.0	1.1641221285181516E-4	6.98473277110891E-5
12	0.0	0.0	0.0	1.1641221285181516E-4	2.3282442570363033E-4
13	0.0	0.0	0.0	1.1641221285181516E-4	2.793893108443564E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1665	0.0	15.978174	1
GTATCAA	5580	0.0	13.451671	1
GTATACG	60	4.0898795E-4	12.668409	1
TCCAACG	1040	0.0	12.240203	18
CCAACGA	800	0.0	11.399697	19
CGCGCCA	125	1.8366336E-8	11.399297	10
ACCGTCG	525	0.0	11.21796	8
CCGTCGT	500	0.0	11.209045	9
TACCGTC	540	0.0	11.08278	7
CGTCGTA	520	0.0	10.960862	10
ATACCGT	590	0.0	10.788103	6
GCTCGTA	330	0.0	10.650608	9
ACTGTTC	2495	0.0	10.355711	8
CCAACGT	370	0.0	10.269998	19
AGCCCCG	520	0.0	10.230618	16
TGTAGAA	3010	0.0	10.195516	2
CGCATCG	345	0.0	10.188016	13
ACGCATC	280	0.0	10.177943	10
CGGTCCA	585	0.0	10.067755	10
GAACAGT	1650	0.0	10.01815	6
>>END_MODULE