FastQCFastQC Report
Thu 2 Feb 2017
SRR4050215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295082
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78330.18237137265365364No Hit
GATATACACTGTTCTACAAATCCCG61370.14288435005431793No Hit
TTGTAGAACAGTGTATATCAATGAG60910.14181335769608125No Hit
TATCAACGCAGAGTACTTTTTTTTT51190.11918282351768836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15400.015.717751
GTATCAA56350.013.9182051
CGCGCCA1251.03682396E-1012.91762410
CGTTATT2100.011.76442
CCAACGA7800.011.68716419
GACCGTC1251.8422725E-811.3969757
GCTCGTA3800.011.24793159
TCCAACG11650.011.16675918
TGCGAAA600.00588707811.08116712
GCGTTAT1900.011.0343411
CCGTTAT700.001454021810.8910381
CCGTCGT4750.010.7980149
AACCGCG4150.010.7561817
GTATTAG7800.010.75141051
TGTAGAA31950.010.7362362
ACGCATC1951.8189894E-1210.71597910
CGTCGTA4800.010.68553510
CGAGCCG6850.010.67649515
AGACCGT1355.7207217E-810.5525096
TTGTAGA16100.010.5358951
Produced by FastQC (version 0.11.5)