##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050215_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4295082
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.80333344043257	31.0	31.0	34.0	27.0	34.0
2	30.71963212809441	31.0	31.0	34.0	27.0	34.0
3	30.993547736690477	31.0	31.0	34.0	27.0	34.0
4	34.64304686150346	37.0	35.0	37.0	32.0	37.0
5	34.596932025977615	37.0	35.0	37.0	32.0	37.0
6	34.56202954914481	37.0	35.0	37.0	31.0	37.0
7	34.5111595075484	37.0	35.0	37.0	31.0	37.0
8	34.49525736644842	37.0	35.0	37.0	31.0	37.0
9	36.09110280083128	39.0	37.0	39.0	32.0	39.0
10	35.97043851549284	39.0	37.0	39.0	31.0	39.0
11	35.97061872159833	39.0	37.0	39.0	31.0	39.0
12	35.893592019896246	39.0	37.0	39.0	31.0	39.0
13	35.91006714190789	39.0	37.0	39.0	31.0	39.0
14	37.05331120569991	40.0	37.0	41.0	31.0	41.0
15	37.0341397440142	40.0	37.0	41.0	31.0	41.0
16	37.012750396849235	40.0	37.0	41.0	31.0	41.0
17	36.993945633634006	40.0	37.0	41.0	31.0	41.0
18	36.96383631325316	40.0	37.0	41.0	31.0	41.0
19	37.029193389090125	40.0	37.0	41.0	31.0	41.0
20	36.9812390077768	40.0	37.0	41.0	31.0	41.0
21	36.93059596999545	40.0	37.0	41.0	31.0	41.0
22	36.87292140173342	40.0	37.0	41.0	31.0	41.0
23	36.77989779939009	40.0	37.0	41.0	31.0	41.0
24	36.7196791586284	40.0	37.0	41.0	31.0	41.0
25	36.5001902175558	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8221737016861113
1101	2	0.9703838826101574
1101	3	0.92968884061683
1101	4	0.8909645443781358
1101	5	0.8722182181121951
1101	6	1.0085302913393335
1101	7	0.956538678583108
1101	8	0.9406249517251055
1101	9	1.0354782550787363
1101	10	1.1317203891381666
1101	11	1.0938792127865327
1101	12	1.1119725336908104
1101	13	1.0887676620995563
1101	14	1.3150283796696698
1101	15	1.2520721499985115
1101	16	1.316880034658503
1101	17	1.322227259138998
1101	18	1.37917878436172
1101	19	1.3409896598700897
1101	20	1.4063793794450348
1101	21	1.3832827695770078
1101	22	1.4009510385689268
1101	23	1.4061157970358735
1101	24	1.4688918566237916
1101	25	1.4746508609671451
1102	1	0.6833101860196216
1102	2	0.8449975712758899
1102	3	0.760497189640791
1102	4	0.6763144856388621
1102	5	0.7483314481287664
1102	6	0.8024951121443067
1102	7	0.7562889159541655
1102	8	0.8342926966196131
1102	9	0.9539999564257116
1102	10	0.9786944312051489
1102	11	0.9337707462840115
1102	12	0.9829846194984313
1102	13	1.007475397982013
1102	14	1.184673530058312
1102	15	1.1626085281185325
1102	16	1.1692213983753348
1102	17	1.040630194493609
1102	18	1.1126563105529996
1102	19	1.0750281863993223
1102	20	1.1232315978585916
1102	21	1.1158864484260462
1102	22	1.1745560209471435
1102	23	1.1923119057598655
1102	24	1.156073819642188
1102	25	1.1885680466315378
1103	1	0.46297833183856696
1103	2	0.6313880233969087
1103	3	0.6129327705998904
1103	4	0.533091403026738
1103	5	0.5426680581272478
1103	6	0.597874353074701
1103	7	0.5942311310581658
1103	8	0.6007115318116831
1103	9	0.6668240107723165
1103	10	0.677017772913814
1103	11	0.719954876253496
1103	12	0.7724637815733644
1103	13	0.7226816466657198
1103	14	0.8564051912638746
1103	15	0.7804111298592318
1103	16	0.8560537594844035
1103	17	0.819221424394037
1103	18	0.7974725920033521
1103	19	0.8765891328608717
1103	20	0.8905051087881546
1103	21	0.8266142207345482
1103	22	0.8447313886536207
1103	23	0.8999479069549423
1103	24	0.7848574128067369
1103	25	0.9079888997399621
1104	1	0.5542414523236658
1104	2	0.6869145861102162
1104	3	0.5897288384547856
1104	4	0.5724394523683713
1104	5	0.6315344221503025
1104	6	0.7048431196385465
1104	7	0.623668809141158
1104	8	0.6518881266886183
1104	9	0.7136798264925659
1104	10	0.7806626084776624
1104	11	0.6904783155377316
1104	12	0.775119390617462
1104	13	0.7643130625938781
1104	14	0.8368533424480944
1104	15	0.8901835051586744
1104	16	0.8969732974305415
1104	17	0.8169384049738682
1104	18	0.843018281981017
1104	19	0.8364767834162379
1104	20	0.8788920200394017
1104	21	0.8431067594350523
1104	22	0.8846888217845716
1104	23	0.8813508457555983
1104	24	0.8979703830916463
1104	25	0.8870433114980756
1105	1	0.4089500511308195
1105	2	0.5500194651555255
1105	3	0.5237046934636851
1105	4	0.41217036735651646
1105	5	0.5008398400311478
1105	6	0.6207585734342018
1105	7	0.5530227514775987
1105	8	0.5678431401440562
1105	9	0.6167905704634791
1105	10	0.726325278107737
1105	11	0.7011724299463324
1105	12	0.7246297007507749
1105	13	0.6541886536450718
1105	14	0.7873704150167882
1105	15	0.7136225881245508
1105	16	0.8423333974752722
1105	17	0.8020279159155166
1105	18	0.789283901607881
1105	19	0.703913883042091
1105	20	0.7511611415203205
1105	21	0.8066649210976635
1105	22	0.7753448404468415
1105	23	0.7509591937915943
1105	24	0.782363201035416
1105	25	0.8637980966200942
1106	1	0.33574463718172964
1106	2	0.5558127478716592
1106	3	0.3801637028071738
1106	4	0.365636427585585
1106	5	0.4338762408580834
1106	6	0.44825271984837656
1106	7	0.42996736673983094
1106	8	0.4662427153345021
1106	9	0.5426984546838725
1106	10	0.5460720749542887
1106	11	0.5571029625374777
1106	12	0.5686478342154828
1106	13	0.5548939981840562
1106	14	0.6384770927418089
1106	15	0.684327116479416
1106	16	0.6091080731626946
1106	17	0.5032129679908195
1106	18	0.5318113865340734
1106	19	0.48708435860077515
1106	20	0.5775630632647051
1106	21	0.6432907795431788
1106	22	0.5756913888741266
1106	23	0.6740828957979517
1106	24	0.699267338882116
1106	25	0.689612592246867
1107	1	0.22408214393461634
1107	2	0.3928122654211137
1107	3	0.3584829299847776
1107	4	0.36993846454227963
1107	5	0.4181245257613284
1107	6	0.4024628766428009
1107	7	0.41162937605425043
1107	8	0.38619349582712204
1107	9	0.38580686377078877
1107	10	0.3247713480977339
1107	11	0.407197364701922
1107	12	0.38345069775133567
1107	13	0.37393504873347183
1107	14	0.37905863209524426
1107	15	0.44171787090884607
1107	16	0.42991240788216345
1107	17	0.4114790912601194
1107	18	0.4267556920448996
1107	19	0.38346947548487265
1107	20	0.4472345073139792
1107	21	0.47085029238363774
1107	22	0.35523454464838977
1107	23	0.39598178651929317
1107	24	0.40706760223842764
1107	25	0.3495072125503782
1108	1	0.13789547463094465
1108	2	0.2847622922074038
1108	3	0.2129984578534021
1108	4	0.25472598265813673
1108	5	0.28297265132361105
1108	6	0.2716566086280423
1108	7	0.28376575073622945
1108	8	0.23896396254826868
1108	9	0.2798488377107091
1108	10	0.3543978645956045
1108	11	0.33109249955007414
1108	12	0.3646745876485227
1108	13	0.39512430776800755
1108	14	0.36671347694486656
1108	15	0.38380969708769896
1108	16	0.36619616173496894
1108	17	0.4067077590194401
1108	18	0.3828343560764509
1108	19	0.3748518556278526
1108	20	0.46345802672591674
1108	21	0.43051441508212207
1108	22	0.4274458778678678
1108	23	0.4645336785808283
1108	24	0.48765579718066476
1108	25	0.5333912552946174
1109	1	0.13267321391097298
1109	2	0.21283205650199477
1109	3	0.20328458173017694
1109	4	0.19724480167952407
1109	5	0.2456222788401945
1109	6	0.24969858900411168
1109	7	0.29683393496991073
1109	8	0.2766611690289267
1109	9	0.31409838494886344
1109	10	0.3430500336720357
1109	11	0.28316408870873033
1109	12	0.3616279662863846
1109	13	0.33346318371409467
1109	14	0.4377065700239129
1109	15	0.504450358644327
1109	16	0.44216073400610867
1109	17	0.37666357399785255
1109	18	0.4178316866237779
1109	19	0.4082415804846846
1109	20	0.4162020274543039
1109	21	0.4269460370607021
1109	22	0.4171934170824301
1109	23	0.41015465137402174
1109	24	0.42964577406785054
1109	25	0.3655260362613362
1110	1	-0.057071731975149476
1110	2	0.08353554191807078
1110	3	-0.03703271637084171
1110	4	0.003202547824336932
1110	5	0.044083078265082065
1110	6	-0.03786007556333715
1110	7	0.0041653899012956686
1110	8	0.020563962479542397
1110	9	0.06066075153488981
1110	10	0.02300611020046972
1110	11	0.03408644068979072
1110	12	-0.027720216043206847
1110	13	-0.0768171292771811
1110	14	-0.011061378018212054
1110	15	-0.03826157206425762
1110	16	-0.06826820925165578
1110	17	-0.11648642222730388
1110	18	-0.06567832522856065
1110	19	-0.10087535429604344
1110	20	-0.08087612298621849
1110	21	-0.09568913655868982
1110	22	-0.10158289824991584
1110	23	-0.06157960419019304
1110	24	-0.12964957632777185
1110	25	-0.012346539905308873
1111	1	-0.464485493842556
1111	2	-0.29617348319898085
1111	3	-0.30440390079431623
1111	4	-0.32794715197211843
1111	5	-0.3462872705735265
1111	6	-0.5098323472963742
1111	7	-0.43959165021789204
1111	8	-0.4014316917952172
1111	9	-0.4245860770937355
1111	10	-0.4395370554016367
1111	11	-0.4787084962457442
1111	12	-0.47843135697523564
1111	13	-0.4156614223786761
1111	14	-0.396084772606784
1111	15	-0.4681764544827516
1111	16	-0.44985588189172176
1111	17	-0.5295057375202674
1111	18	-0.5551645948150821
1111	19	-0.5276375185222406
1111	20	-0.5391876586506754
1111	21	-0.46281594874453447
1111	22	-0.5559948500591076
1111	23	-0.54349466350304
1111	24	-0.5479579238524224
1111	25	-0.5127743389921875
1112	1	-0.5606392256044295
1112	2	-0.4291908656213046
1112	3	-0.48073779070532296
1112	4	-0.4465550182160527
1112	5	-0.4643338408279263
1112	6	-1.3830343844044464
1112	7	-0.6627414039805544
1112	8	-0.5594092402493871
1112	9	-0.5683937153604006
1112	10	-0.6732754809323254
1112	11	-0.5821015014031374
1112	12	-0.5856605364795726
1112	13	-0.529441258198915
1112	14	-0.5500144650746677
1112	15	-0.5402559253146393
1112	16	-0.5301392738533437
1112	17	-0.5793382024264631
1112	18	-0.647306968691197
1112	19	-0.5894079833633725
1112	20	-0.5905582210746516
1112	21	-0.5965283188907904
1112	22	-0.7312579760281537
1112	23	-0.626748942097521
1112	24	-0.622225389717336
1112	25	-0.5654249970191643
1113	1	-0.6909070521465246
1113	2	-0.8090129861852056
1113	3	-0.6632399098307182
1113	4	-0.6680500375311809
1113	5	-0.6543180901910333
1113	6	-1.160768442508271
1113	7	-0.8119040190701199
1113	8	-0.8524953490267748
1113	9	-0.9343240103250352
1113	10	-0.893587523500571
1113	11	-0.9374974431389944
1113	12	-0.9328842511283781
1113	13	-0.88549640716451
1113	14	-1.1018894017969885
1113	15	-1.0922589700093042
1113	16	-1.0818487678672923
1113	17	-1.0475647533597794
1113	18	-1.0383204832879258
1113	19	-1.084397997585235
1113	20	-1.0758005834492153
1113	21	-1.1513632610840574
1113	22	-1.186991056929564
1113	23	-1.1488363560741703
1113	24	-1.132421491682038
1113	25	-1.1589070598088256
1114	1	-0.9218605388681134
1114	2	-0.9186087718891578
1114	3	-0.9855927019557917
1114	4	-0.8329808091513868
1114	5	-1.1592480682592239
1114	6	-1.6497013007800234
1114	7	-1.2331310622533707
1114	8	-1.4330536533463132
1114	9	-1.4656460976241448
1114	10	-1.622660678589689
1114	11	-1.3875487819075119
1114	12	-1.4323502838496083
1114	13	-1.529754534315252
1114	14	-1.8005229348481393
1114	15	-1.654043228365559
1114	16	-1.638961115175377
1114	17	-1.6055905346941373
1114	18	-1.813862162042163
1114	19	-1.6171160643074955
1114	20	-1.6130620968555434
1114	21	-1.609661704869282
1114	22	-1.5360952068019103
1114	23	-1.5399009422469092
1114	24	-1.6247743748704622
1114	25	-1.596896028423913
1115	1	-1.2781047198516404
1115	2	-1.9599002196809998
1115	3	-1.592178182756502
1115	4	-1.218619150978391
1115	5	-1.4551900952915062
1115	6	-1.9607456563977337
1115	7	-1.6291424860477122
1115	8	-1.5885292269771796
1115	9	-1.6586548719380971
1115	10	-1.6914583066853908
1115	11	-1.7788933414787635
1115	12	-1.760652685212058
1115	13	-1.7845256416022153
1115	14	-1.9025257475709836
1115	15	-1.8561656943934963
1115	16	-1.9471688797418807
1115	17	-1.8337714930926197
1115	18	-1.9964197303004383
1115	19	-2.001248462978701
1115	20	-1.977390185228316
1115	21	-2.202544625988473
1115	22	-2.241202595556487
1115	23	-2.19487240732321
1115	24	-2.208329849611353
1115	25	-2.21523193457449
1116	1	-0.9482859417632241
1116	2	-1.0112572254227032
1116	3	-1.1054533367933885
1116	4	-1.0147025033507617
1116	5	-0.9809193659532269
1116	6	-1.269508096389771
1116	7	-1.2383217187515143
1116	8	-1.2676949757700768
1116	9	-1.3505279037109332
1116	10	-1.4787935642623822
1116	11	-1.4270006633510732
1116	12	-1.358372997067093
1116	13	-1.4179489202871238
1116	14	-1.704341528772403
1116	15	-1.6617337005083712
1116	16	-1.7000990000524894
1116	17	-1.668124451006527
1116	18	-1.749614621545085
1116	19	-1.754578071367746
1116	20	-1.6625426950440527
1116	21	-1.7923682460740054
1116	22	-1.7283466053678964
1116	23	-1.6936800771929867
1116	24	-1.8419924480749472
1116	25	-2.037534891424791
1201	1	0.7279623141782103
1201	2	0.8515791779373139
1201	3	0.8444906881767018
1201	4	0.7530515817223389
1201	5	0.8439383286310616
1201	6	0.8723778601782755
1201	7	0.8374542099508275
1201	8	0.8611742884774145
1201	9	1.0044785195708883
1201	10	1.0333420996104152
1201	11	1.024762721177808
1201	12	1.0299264104434442
1201	13	1.0136541919774587
1201	14	1.2383233242832148
1201	15	1.2144524897140272
1201	16	1.1471034483406726
1201	17	1.1873682693197054
1201	18	1.2437151385839584
1201	19	1.185397981958772
1201	20	1.1689397531410677
1201	21	1.2491379982282353
1201	22	1.231036527812165
1201	23	1.2570038182311123
1201	24	1.247441543043685
1201	25	1.2764801903076943
1202	1	0.5628058597520749
1202	2	0.743059842088563
1202	3	0.7735837101736145
1202	4	0.7332670280451836
1202	5	0.706963988295847
1202	6	0.8148648325618595
1202	7	0.7819922541996149
1202	8	0.8276756742544435
1202	9	0.9227414603052821
1202	10	0.9172775610904509
1202	11	0.9387274331337352
1202	12	0.9494693817171864
1202	13	0.9654758554676306
1202	14	1.1865651321229151
1202	15	1.1779190390225907
1202	16	1.1866480670334596
1202	17	1.1609234441488283
1202	18	1.129942223231069
1202	19	1.132685024925948
1202	20	1.0934586681129161
1202	21	1.1404872555187708
1202	22	1.078321719483391
1202	23	1.1954044649192497
1202	24	1.2305515140064642
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2211	8	-0.1699986661430941
2211	9	-0.1823746659940042
2211	10	-0.19102444085124404
2211	11	-0.17369694686393444
2211	12	-0.24171998637942238
2211	13	-0.27086993456590136
2211	14	-0.36910896904972645
2211	15	-0.4009180795861482
2211	16	-0.3609123071586069
2211	17	-0.35515764697655783
2211	18	-0.35636301130391246
2211	19	-0.342421541931202
2211	20	-0.28686898210054323
2211	21	-0.29062901523260365
2211	22	-0.33803975392032015
2211	23	-0.31540082875319797
2211	24	-0.4252941431349626
2211	25	-0.30935192675081424
2212	1	-0.34416496309500744
2212	2	-0.3758789425499778
2212	3	-0.39171839905367634
2212	4	-0.341718573101673
2212	5	-0.36397058733918897
2212	6	-0.3013789218917253
2212	7	-0.4426387210905034
2212	8	-0.4461861145346191
2212	9	-0.48325257990295967
2212	10	-0.5462921473719575
2212	11	-0.5314405994939975
2212	12	-0.6006565234454868
2212	13	-0.4981453192448484
2212	14	-0.6131014367578729
2212	15	-0.6272930884845778
2212	16	-0.665430537838148
2212	17	-0.6053146958047435
2212	18	-0.5464624661864903
2212	19	-0.6284057414205577
2212	20	-0.5848882640073114
2212	21	-0.6325067889003506
2212	22	-0.6446894959482492
2212	23	-0.7707213148529277
2212	24	-0.7366144220733943
2212	25	-0.6154306173982746
2213	1	-0.4664902371148152
2213	2	-0.83210025425754
2213	3	-0.5955101156780174
2213	4	-0.5288065521052872
2213	5	-0.5514649936532123
2213	6	-0.47085297379735636
2213	7	-0.515469267808399
2213	8	-0.5158721128541472
2213	9	-0.6457475054620971
2213	10	-0.7254159448474766
2213	11	-0.6334528159153621
2213	12	-0.6430111584161722
2213	13	-0.6806725324091261
2213	14	-0.712991545610528
2213	15	-0.7392016446999392
2213	16	-0.723375554501196
2213	17	-0.7115781135704822
2213	18	-0.7561885879919501
2213	19	-0.7224474509642818
2213	20	-0.7269509888168386
2213	21	-0.776948505996728
2213	22	-0.7508956079352558
2213	23	-0.8452113689488243
2213	24	-0.8130241725525096
2213	25	-0.864716985797962
2214	1	-0.7319784479794507
2214	2	-0.8031307505034988
2214	3	-0.8127103027305651
2214	4	-0.6994032274689062
2214	5	-0.8238551837861863
2214	6	-0.8166815906105569
2214	7	-0.8643377483404606
2214	8	-0.8768670171567337
2214	9	-0.9816632650104964
2214	10	-1.0003656373612984
2214	11	-1.0795351413051435
2214	12	-1.0924864463249335
2214	13	-0.9613711217211574
2214	14	-1.16139042774887
2214	15	-1.305821709054925
2214	16	-1.2734796210384545
2214	17	-1.1783958078509045
2214	18	-1.2209532320910768
2214	19	-1.2387674889686053
2214	20	-1.2619988096917538
2214	21	-1.4530640480594315
2214	22	-1.2832086760053159
2214	23	-1.4050562712101566
2214	24	-1.411076265202908
2214	25	-1.3803434448264014
2215	1	-1.4660050962601296
2215	2	-3.2062102984026275
2215	3	-1.7965570065592509
2215	4	-1.4894421806797524
2215	5	-1.3509922923076232
2215	6	-1.306673071189607
2215	7	-1.358350076073087
2215	8	-1.4233119253513067
2215	9	-1.6391477390821478
2215	10	-1.6523438412051306
2215	11	-1.6199469223186753
2215	12	-1.6832766357444058
2215	13	-1.7891508922143444
2215	14	-1.9881041079977066
2215	15	-1.9297420048389
2215	16	-1.9831093945169727
2215	17	-1.9737704253470554
2215	18	-1.8998461829190632
2215	19	-1.8614048835709625
2215	20	-1.8602887973394218
2215	21	-1.8495174921767372
2215	22	-1.8943897446788398
2215	23	-2.0989878977189846
2215	24	-2.13584991323426
2215	25	-2.1120852711178273
2216	1	-1.1641600234044667
2216	2	-1.9276082718289942
2216	3	-1.3465449198302721
2216	4	-1.1595318415600318
2216	5	-1.2520641919876212
2216	6	-1.2555451528884944
2216	7	-1.287638159594536
2216	8	-1.3476222268415512
2216	9	-1.5702377666351524
2216	10	-1.4426293603860216
2216	11	-1.575144663573262
2216	12	-1.6071134046545836
2216	13	-1.803548329176465
2216	14	-1.789615870612863
2216	15	-1.8147211983454312
2216	16	-1.8154660009838821
2216	17	-1.7742861245498958
2216	18	-2.062101063981352
2216	19	-1.9632540605853634
2216	20	-2.0494996005510444
2216	21	-1.971430417249529
2216	22	-1.939411958946863
2216	23	-2.106501413316934
2216	24	-2.046468441412621
2216	25	-2.2308464132241568
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54829.0
3	2.0
4	1032.0
5	8206.0
6	9887.0
7	7930.0
8	6390.0
9	5736.0
10	5183.0
11	4778.0
12	4554.0
13	4233.0
14	4271.0
15	4594.0
16	4458.0
17	4925.0
18	5490.0
19	6515.0
20	8540.0
21	11501.0
22	14806.0
23	18380.0
24	21946.0
25	25475.0
26	29680.0
27	33960.0
28	39951.0
29	49226.0
30	63819.0
31	83086.0
32	108748.0
33	144749.0
34	195857.0
35	268578.0
36	391689.0
37	674057.0
38	1968021.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.457228800562085	19.31184601032135	13.515393502135606	25.71553168698096
2	15.046598946357365	21.450673807375498	39.33018598829933	24.172541257967808
3	18.20740558620301	25.589755911528584	30.13611847224337	26.06672003002504
4	12.477643034521808	18.101237648082154	37.57348520936271	31.84763410803333
5	13.630166390905302	37.61562818035003	34.48019468138905	14.274010747355614
6	30.64992268839944	38.324341780371725	18.166212141355548	12.859523389873287
7	27.08614722293689	31.88586968543033	22.37925546838529	18.648727623247492
8	25.364726896904656	35.40360176323374	20.809543422296716	18.422127917564886
9	26.609819312778964	15.160575030520468	19.867304947843355	38.36230070885721
10	15.19318857375983	28.619484164491176	32.80609235542497	23.38123490632403
11	34.92161505810474	22.238831339075123	24.403014787546887	18.43653881527325
12	23.51811181151645	24.701536048521696	31.04774864389604	20.732603496065806
13	28.771447839171827	21.236981798502786	26.049163882402365	23.942406479923026
14	22.443102708028906	21.863419490692753	26.61364603677078	29.079831764507563
15	23.747484699778934	29.529607129957743	23.385090513548565	23.33781765671476
16	23.688970395452234	27.367197261354637	26.10924497715545	22.834587366037688
17	22.37460411778046	27.305733068395128	26.827763934285763	23.49189887953865
18	22.603713603936416	27.052555334915144	28.520819087358934	21.822911973789502
19	24.84180674543442	25.76341036646911	26.38153812482465	23.01324476327183
20	24.435196264219485	26.672734329724634	26.38048065856729	22.511588747488588
21	25.635788165916335	25.665590372397197	25.430455619857955	23.268165841828512
22	24.282982257381818	26.314189112105424	26.324922318130362	23.077906312382396
23	23.754485788319453	25.830876379788094	26.245828534095715	24.168809297796738
24	23.71626858794376	26.594086363870257	26.520604220659706	23.16904082752628
25	23.97582568640727	26.058563361996306	26.748998596498417	23.216612355098007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	255.0
1	255.0
2	572.5
3	890.0
4	890.0
5	890.0
6	2429.0
7	3968.0
8	3968.0
9	3968.0
10	5803.5
11	7639.0
12	7639.0
13	7639.0
14	12898.5
15	18158.0
16	18158.0
17	18158.0
18	32178.5
19	46199.0
20	46199.0
21	46199.0
22	75528.0
23	104857.0
24	104857.0
25	104857.0
26	155203.5
27	205550.0
28	205550.0
29	205550.0
30	261764.5
31	317979.0
32	317979.0
33	317979.0
34	350916.0
35	383853.0
36	383853.0
37	383853.0
38	404015.0
39	424177.0
40	424177.0
41	424177.0
42	456262.5
43	488348.0
44	488348.0
45	488348.0
46	508454.0
47	528560.0
48	528560.0
49	528560.0
50	517495.5
51	506431.0
52	506431.0
53	506431.0
54	453626.5
55	400822.0
56	400822.0
57	400822.0
58	357122.0
59	313422.0
60	313422.0
61	313422.0
62	272663.0
63	231904.0
64	231904.0
65	231904.0
66	189960.5
67	148017.0
68	148017.0
69	148017.0
70	112043.0
71	76069.0
72	76069.0
73	76069.0
74	59600.5
75	43132.0
76	43132.0
77	43132.0
78	34605.5
79	26079.0
80	26079.0
81	26079.0
82	18713.0
83	11347.0
84	11347.0
85	11347.0
86	8309.5
87	5272.0
88	5272.0
89	5272.0
90	3587.0
91	1902.0
92	1902.0
93	1902.0
94	1055.0
95	208.0
96	208.0
97	208.0
98	126.0
99	44.0
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3226946540252317
2	0.010640076254655908
3	0.0
4	0.0
5	0.04933549580659927
6	0.002910305321295379
7	0.0038881679092506263
8	4.423664088368977E-4
9	0.009848473207263562
10	0.015156870113306334
11	0.005867175527731485
12	0.009918320534974653
13	0.016530534224957753
14	0.003492366385554455
15	0.0200927479382233
16	0.009406106798426666
17	0.00568091598716858
18	2.095419831332673E-4
19	0.004120992334954257
20	0.004260686990376435
21	0.0022816793718955773
22	0.0
23	0.0030034350915768312
24	0.0037484732538284483
25	0.0025610686827399337
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4295082.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.77782081256693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.35694322788913	51.934285765950165
2	7.093316278611836	8.33859346382221
3	1.7351891469354508	3.059719102634484
4	0.7491341398205321	1.7612988893979078
5	0.4316758303829062	1.2686482303681248
6	0.28342360596811467	0.9995413155387249
7	0.2078271415100931	0.8550938538567767
8	0.14466981872274842	0.6802701345497797
9	0.11778656405166024	0.623091380036078
>10	0.731004081042704	7.921541450209768
>50	0.06484012001063622	2.6758297438830527
>100	0.0665389023194982	8.394817759029223
>500	0.011185668093381434	4.536747245538384
>1k	0.006306812686958964	6.363462308116414
>5k	1.586619543889042E-4	0.5870593570688466
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7833	0.18237137265365364	No Hit
GATATACACTGTTCTACAAATCCCG	6137	0.14288435005431793	No Hit
TTGTAGAACAGTGTATATCAATGAG	6091	0.14181335769608125	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5119	0.11918282351768836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6564885140726065E-5
2	0.0	0.0	0.0	0.0	4.6564885140726065E-5
3	0.0	0.0	0.0	2.3282442570363033E-5	4.6564885140726065E-5
4	0.0	0.0	0.0	2.3282442570363033E-5	4.6564885140726065E-5
5	0.0	0.0	0.0	2.3282442570363033E-5	4.6564885140726065E-5
6	0.0	0.0	0.0	2.3282442570363033E-5	6.98473277110891E-5
7	0.0	0.0	0.0	2.3282442570363033E-5	6.98473277110891E-5
8	0.0	0.0	0.0	2.3282442570363033E-5	6.98473277110891E-5
9	0.0	0.0	0.0	4.6564885140726065E-5	6.98473277110891E-5
10	0.0	0.0	0.0	4.6564885140726065E-5	6.98473277110891E-5
11	0.0	0.0	0.0	6.98473277110891E-5	6.98473277110891E-5
12	0.0	0.0	0.0	6.98473277110891E-5	1.8625954056290426E-4
13	0.0	0.0	0.0	6.98473277110891E-5	2.793893108443564E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1540	0.0	15.71775	1
GTATCAA	5635	0.0	13.918205	1
CGCGCCA	125	1.03682396E-10	12.917624	10
CGTTATT	210	0.0	11.7644	2
CCAACGA	780	0.0	11.687164	19
GACCGTC	125	1.8422725E-8	11.396975	7
GCTCGTA	380	0.0	11.2479315	9
TCCAACG	1165	0.0	11.166759	18
TGCGAAA	60	0.005887078	11.081167	12
GCGTTAT	190	0.0	11.034341	1
CCGTTAT	70	0.0014540218	10.891038	1
CCGTCGT	475	0.0	10.798014	9
AACCGCG	415	0.0	10.756181	7
GTATTAG	780	0.0	10.7514105	1
TGTAGAA	3195	0.0	10.736236	2
ACGCATC	195	1.8189894E-12	10.715979	10
CGTCGTA	480	0.0	10.685535	10
CGAGCCG	685	0.0	10.676495	15
AGACCGT	135	5.7207217E-8	10.552509	6
TTGTAGA	1610	0.0	10.535895	1
>>END_MODULE