FastQCFastQC Report
Thu 2 Feb 2017
SRR4050214_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050214_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4260211
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN140830.33057048113344617No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN61750.14494587239927786No Hit
GATATACACTGTTCTACAAATCCCG56640.13295116133919188No Hit
TTGTAGAACAGTGTATATCAATGAG55610.13053344071455616No Hit
GTATCAACGCAGAGTACTTTTTTTT47270.1109569455597387No Hit
GTGTATATCAATGAGTTACAATGAA44720.1049713265375823No Hit
GTTCTACAGTGTGGTTTTTATCATT42850.10058187258800091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.015.4620671
AATCGTA1257.2759576E-1213.67954113
GTATCAA55350.013.6534071
CCAACGA7350.011.37349819
CGAACGA2300.011.15166816
CGTCGTA5300.011.11270310
TCCAACG10550.011.07532718
CGCATCG3850.010.85677813
CTCGACG1053.4716104E-610.85677813
GTCGATA1053.4721033E-610.856659
AACCGCG3700.010.7832947
ACTGTTC26750.010.7602598
CGTTCAA803.775151E-410.68701514
GTATTAG7950.010.6417831
CGACCAT4750.010.59951910
CGTCTTA2800.010.51737915
TGAGTTA35300.010.44145312
GAACAGT16500.010.4207396
GATATAC26650.010.4154291
CCGTCGT5700.010.3328659
Produced by FastQC (version 0.11.5)