##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050214_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4260211
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.050015128358666	31.0	31.0	34.0	26.0	34.0
2	30.11714607562865	31.0	31.0	34.0	26.0	34.0
3	30.27060068151554	31.0	31.0	34.0	26.0	34.0
4	33.72323812130432	37.0	35.0	37.0	28.0	37.0
5	33.750045009507744	37.0	35.0	37.0	29.0	37.0
6	33.83321060858253	37.0	35.0	37.0	30.0	37.0
7	33.77976396004799	37.0	35.0	37.0	29.0	37.0
8	33.73267568202608	37.0	35.0	37.0	29.0	37.0
9	35.265546471759265	39.0	35.0	39.0	29.0	39.0
10	35.1271178821894	39.0	35.0	39.0	29.0	39.0
11	35.17417916624317	39.0	35.0	39.0	29.0	39.0
12	35.09318622950835	39.0	35.0	39.0	29.0	39.0
13	35.11124801095533	39.0	35.0	39.0	29.0	39.0
14	36.16965826340526	40.0	36.0	41.0	28.0	41.0
15	36.158363048215215	40.0	36.0	41.0	28.0	41.0
16	36.160306613921236	40.0	36.0	41.0	28.0	41.0
17	36.13163643772574	40.0	36.0	41.0	28.0	41.0
18	36.07572582672548	40.0	36.0	41.0	27.0	41.0
19	36.12895910554665	40.0	37.0	41.0	27.0	41.0
20	36.09419768175802	40.0	37.0	41.0	27.0	41.0
21	36.01425445828857	40.0	37.0	41.0	27.0	41.0
22	35.92421736857634	40.0	36.0	41.0	27.0	41.0
23	35.853905358208785	40.0	36.0	41.0	27.0	41.0
24	35.79111339790447	40.0	36.0	41.0	27.0	41.0
25	35.45808716986084	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.307820889080297
1101	2	1.3959759742681896
1101	3	1.3912104264715595
1101	4	1.435058366329777
1101	5	1.4541161198856543
1101	6	1.4126229533769035
1101	7	1.3496559607321643
1101	8	1.3971091946721614
1101	9	1.5195519721087436
1101	10	1.6166256693977203
1101	11	1.5775555709398006
1101	12	1.577557111121159
1101	13	1.6283362094902785
1101	14	1.8895450091860155
1101	15	1.8729646866581504
1101	16	1.9123338713198592
1101	17	1.8360897242960448
1101	18	1.8975082275922048
1101	19	1.85751893632942
1101	20	1.8997829167430353
1101	21	1.8467084341013944
1101	22	1.9270378475807135
1101	23	1.9756367076145978
1101	24	1.922367490723481
1101	25	1.8992249282235818
1102	1	1.0940252716424936
1102	2	1.1689822022474026
1102	3	1.1801790938672845
1102	4	1.2117725429839155
1102	5	1.2379766719070986
1102	6	1.1346528042840802
1102	7	1.147723353356291
1102	8	1.1673049821783366
1102	9	1.2960956761089193
1102	10	1.3589562529330266
1102	11	1.2975646807828056
1102	12	1.2956384649839805
1102	13	1.3058117183646232
1102	14	1.5437061927648514
1102	15	1.5574639383977527
1102	16	1.4733368030452283
1102	17	1.5298665964799625
1102	18	1.542019176585839
1102	19	1.5551543525960128
1102	20	1.5837427876150514
1102	21	1.6301454997077656
1102	22	1.643852111223076
1102	23	1.6478194785566274
1102	24	1.6453565029860542
1102	25	1.6111679189338304
1103	1	0.9925657152591363
1103	2	1.0448697442448598
1103	3	1.0749976494119764
1103	4	1.1296067931711349
1103	5	1.105594462411645
1103	6	1.0685294053092775
1103	7	1.0510446547852013
1103	8	1.0128047934441113
1103	9	1.2115166656936864
1103	10	1.2026171333092037
1103	11	1.1961441224569143
1103	12	1.1041975271035014
1103	13	1.1947554825810798
1103	14	1.372767124218548
1103	15	1.3359792361604903
1103	16	1.39929299799482
1103	17	1.482495650231563
1103	18	1.4311162435109495
1103	19	1.4750155078178722
1103	20	1.4103379543349632
1103	21	1.4612793503958343
1103	22	1.493378077264012
1103	23	1.5157922273922964
1103	24	1.4818112205699592
1103	25	1.4666358558652632
1104	1	0.9300266747908061
1104	2	1.0340356659566012
1104	3	1.0100573832791504
1104	4	1.1336875670919895
1104	5	1.0498006451288333
1104	6	1.0168450640760796
1104	7	0.9961552610856259
1104	8	1.0599502413422215
1104	9	1.2569455033633687
1104	10	1.2034521701928327
1104	11	1.2381128767065022
1104	12	1.2131368747682103
1104	13	1.233980034660675
1104	14	1.3754207614191785
1104	15	1.3772168385128367
1104	16	1.3985347044729153
1104	17	1.3515289513887865
1104	18	1.4037004717190698
1104	19	1.4354009969132875
1104	20	1.357998034188185
1104	21	1.3812769873467872
1104	22	1.4251220743391784
1104	23	1.5660187791464182
1104	24	1.4871600155264062
1104	25	1.4970567684034677
1105	1	0.7154995669282229
1105	2	0.8762394517558114
1105	3	0.8429317310218742
1105	4	0.9412807687739786
1105	5	0.8493232606758951
1105	6	0.8386196158613046
1105	7	0.800266218374027
1105	8	0.8270490083719508
1105	9	0.9548512022393112
1105	10	0.992768540655419
1105	11	0.8602978903841674
1105	12	0.8808394237941357
1105	13	0.9689348975856333
1105	14	1.0899365658658553
1105	15	1.0837560230717358
1105	16	1.1130111582427418
1105	17	0.9805582362153231
1105	18	1.066898081310704
1105	19	1.0921944291004522
1105	20	1.052777229353282
1105	21	1.1342471183343648
1105	22	1.1492232811108778
1105	23	1.097708803564963
1105	24	1.025591788055202
1105	25	1.0044639391003685
1106	1	0.562652130252566
1106	2	0.725782839595805
1106	3	0.6822008993342017
1106	4	0.728567780617162
1106	5	0.6907121547149302
1106	6	0.6594544684884269
1106	7	0.6139601012889955
1106	8	0.6007320413059247
1106	9	0.6899764304850962
1106	10	0.7506961892599406
1106	11	0.8442409334606609
1106	12	0.7677614333127138
1106	13	0.7914027994739499
1106	14	0.9599131550496693
1106	15	0.9470015189905823
1106	16	0.9464645528412348
1106	17	0.9685748457203687
1106	18	1.0103109635854963
1106	19	0.9981589993954358
1106	20	1.045492105730375
1106	21	1.0732611960264364
1106	22	1.1009102913663043
1106	23	1.0384380314210446
1106	24	1.0204912317839003
1106	25	0.981936586913271
1107	1	0.5372707336617175
1107	2	0.6060793192399565
1107	3	0.576357894513766
1107	4	0.6529329394675116
1107	5	0.5819505931148612
1107	6	0.6063423905173906
1107	7	0.5369293585318786
1107	8	0.5185757569282003
1107	9	0.6265788554514131
1107	10	0.5919948783195608
1107	11	0.5208418596208233
1107	12	0.4707682049901507
1107	13	0.5617253402478255
1107	14	0.7124890656827034
1107	15	0.6714255278821497
1107	16	0.6759781974641896
1107	17	0.7062376832597934
1107	18	0.5869937657619531
1107	19	0.6645020558125339
1107	20	0.7095654863940268
1107	21	0.6775105280613829
1107	22	0.7001372029169133
1107	23	0.6169463785295335
1107	24	0.6143555313957307
1107	25	0.46336532460082225
1108	1	0.473690107581092
1108	2	0.6343965674646128
1108	3	0.5868131215327779
1108	4	0.749061147735631
1108	5	0.7267719249217919
1108	6	0.5889629178910027
1108	7	0.5939478435339041
1108	8	0.6375476562498932
1108	9	0.6935342320775746
1108	10	0.8001145908117024
1108	11	0.7657730281631032
1108	12	0.7151156435248254
1108	13	0.6506035001136397
1108	14	0.837232847062289
1108	15	0.7947600717413863
1108	16	0.7374021120259613
1108	17	0.7758920504964664
1108	18	0.7060535756653792
1108	19	0.730168615563457
1108	20	0.7735279477330295
1108	21	0.8450143581166571
1108	22	0.8442221080614871
1108	23	0.7595687287136101
1108	24	0.696771701774864
1108	25	0.7246825949201465
1109	1	0.4494513816947503
1109	2	0.636649547496468
1109	3	0.4471484041457465
1109	4	0.5543117529362718
1109	5	0.5807566702662754
1109	6	0.35045421895829776
1109	7	0.42266579733347953
1109	8	0.36393262586751973
1109	9	0.44889485288244657
1109	10	0.47376724891299205
1109	11	0.47994530081013664
1109	12	0.5169033085784918
1109	13	0.44412064593985434
1109	14	0.5288819028841374
1109	15	0.4947422012897391
1109	16	0.5279577265162274
1109	17	0.47346138627650447
1109	18	0.4287935975610395
1109	19	0.5128346484953568
1109	20	0.4491212884683762
1109	21	0.5909563527436674
1109	22	0.5303672729143116
1109	23	0.43500467644719265
1109	24	0.44840269439124114
1109	25	0.49529707941553625
1110	1	0.016979531025157257
1110	2	0.17255963507594174
1110	3	0.08103232057994347
1110	4	0.09611229501799556
1110	5	0.18934049238620787
1110	6	0.04762622355379875
1110	7	-0.08178108105484938
1110	8	-0.002126881042478601
1110	9	0.03271671777483931
1110	10	0.12897287083878695
1110	11	0.0272702068996864
1110	12	0.022075545601609292
1110	13	0.08804840651934853
1110	14	0.07292383268963931
1110	15	-0.08576166165039467
1110	16	0.038223350302125425
1110	17	0.022807005628081356
1110	18	0.05727121284352421
1110	19	0.019113403336255885
1110	20	0.034574949934416566
1110	21	0.03865822683794562
1110	22	0.0647577395735297
1110	23	0.010679439144034575
1110	24	0.054591338899577124
1110	25	0.11465057189122518
1111	1	0.06297914536259341
1111	2	0.10053211488622082
1111	3	-0.008949374838731927
1111	4	0.06711996797614717
1111	5	0.119663643076656
1111	6	0.058881670317980195
1111	7	-0.026932813370493136
1111	8	0.07716706866021639
1111	9	0.10860302214894801
1111	10	0.10763623347096285
1111	11	0.12654809692254076
1111	12	0.13706918504740884
1111	13	0.13422646174392128
1111	14	0.025569362745414992
1111	15	0.04308273213985814
1111	16	5.104444210601855E-4
1111	17	0.1414622808587609
1111	18	0.04824196005034764
1111	19	0.025107722008655742
1111	20	0.06355432562155272
1111	21	0.158381988832339
1111	22	0.12553053251878765
1111	23	0.02328902199528926
1111	24	0.020523519850151217
1111	25	0.012789606123092767
1112	1	-0.26067288611800166
1112	2	-0.15609970720255362
1112	3	-0.3083304433903393
1112	4	-0.21121708381114246
1112	5	-0.21521811935468094
1112	6	-0.2967998029774961
1112	7	-0.3965378695411559
1112	8	-0.33246558052393027
1112	9	-0.43525179828795757
1112	10	-0.3800915942337184
1112	11	-0.29366894031528545
1112	12	-0.4427174845847546
1112	13	-0.3887952859989383
1112	14	-0.5372335869963791
1112	15	-0.5168373153080736
1112	16	-0.4374391004448128
1112	17	-0.48449635047217043
1112	18	-0.4312078477716099
1112	19	-0.3684307402426654
1112	20	-0.40270979813229957
1112	21	-0.4287933994043698
1112	22	-0.36466398548385826
1112	23	-0.42413505088177317
1112	24	-0.3852635992399982
1112	25	-0.49706916213034447
1113	1	-0.46617717470389053
1113	2	-0.33444867441135173
1113	3	-0.4538549153131832
1113	4	-0.3485030743808082
1113	5	-0.43844522892019455
1113	6	-0.478476072404149
1113	7	-0.6140655749778929
1113	8	-0.5861629230263503
1113	9	-0.5079635759069916
1113	10	-0.58541987576141
1113	11	-0.5382623587858788
1113	12	-0.507341782665172
1113	13	-0.5676879493111713
1113	14	-0.6697081581564532
1113	15	-0.7405094808890667
1113	16	-0.7442667635592386
1113	17	-0.8385350705321173
1113	18	-0.7698840179237081
1113	19	-0.8352460948058251
1113	20	-0.9379588316982961
1113	21	-0.8338847655789152
1113	22	-0.7846118626133176
1113	23	-0.9281109592625612
1113	24	-1.0014157923491496
1113	25	-0.9749108587846465
1114	1	-0.7058208936426524
1114	2	-0.6634546165532491
1114	3	-0.8289984288297454
1114	4	-0.784559874255919
1114	5	-0.708189359103649
1114	6	-0.8180954645509431
1114	7	-0.7196224547293042
1114	8	-0.8048588359433495
1114	9	-0.9758687862072861
1114	10	-0.8690190328207876
1114	11	-0.9216945398019547
1114	12	-1.1027584840540712
1114	13	-0.9830445415078941
1114	14	-1.041768337217647
1114	15	-1.032157276248526
1114	16	-0.9514893462457721
1114	17	-1.302888660180443
1114	18	-1.0567682562367509
1114	19	-1.0464672051482964
1114	20	-1.0296341197897334
1114	21	-1.1668387804821236
1114	22	-1.0582098333549368
1114	23	-1.2555681017142106
1114	24	-1.183573536948039
1114	25	-1.2099448234986028
1115	1	-1.1095416933559754
1115	2	-1.0252402418903834
1115	3	-1.144569191851943
1115	4	-1.2510098627769821
1115	5	-1.1128715403439386
1115	6	-1.2377849037636892
1115	7	-1.1792559726574652
1115	8	-1.286599440029562
1115	9	-1.859846025263387
1115	10	-1.5658331535222487
1115	11	-1.5257311591539846
1115	12	-1.4754250265233537
1115	13	-1.490544446376063
1115	14	-1.7426122782759563
1115	15	-1.7153028557203953
1115	16	-1.8788241202927978
1115	17	-1.708955113234822
1115	18	-1.5525031401497742
1115	19	-1.661469647917258
1115	20	-1.7710679178653521
1115	21	-1.7488069976142597
1115	22	-1.8806317833247945
1115	23	-1.8222114738571236
1115	24	-2.0782268215570454
1115	25	-1.968905331075156
1116	1	-1.916896754352475
1116	2	-1.996167447584174
1116	3	-1.8727710664346802
1116	4	-2.1260090006492156
1116	5	-1.6996080722188722
1116	6	-1.8482725852293882
1116	7	-1.9379057266183928
1116	8	-1.8777593317421335
1116	9	-2.349873697648704
1116	10	-3.1404316580109892
1116	11	-2.5408150858111966
1116	12	-2.2202421756692274
1116	13	-2.2176918080804953
1116	14	-2.5010347440087273
1116	15	-3.339502060946579
1116	16	-2.862776352214432
1116	17	-2.641711969310208
1116	18	-2.7548455012205793
1116	19	-2.627093182953189
1116	20	-2.7282002781983863
1116	21	-2.650467619401006
1116	22	-2.6815425525092635
1116	23	-2.748298212897005
1116	24	-2.760125259632339
1116	25	-2.860368949527434
1201	1	1.0685430250146553
1201	2	1.1558207395458915
1201	3	1.0753929266133113
1201	4	1.118435832389757
1201	5	1.0991961744733487
1201	6	1.019045489510816
1201	7	0.9701860756595622
1201	8	0.991217827150912
1201	9	1.166330777461397
1201	10	1.188817617481007
1201	11	1.1757112818289528
1201	12	1.1901814965808555
1201	13	1.1606196891421803
1201	14	1.4118718428880612
1201	15	1.4165501613132392
1201	16	1.3886882315451885
1201	17	1.3056085717435835
1201	18	1.4147479862581065
1201	19	1.3846900957586428
1201	20	1.4258936547383172
1201	21	1.4313981400135134
1201	22	1.540494458999973
1201	23	1.5282000442186856
1201	24	1.586922306381858
1201	25	1.649196940674372
1202	1	1.0281813442692354
1202	2	1.0449393187720375
1202	3	0.9413779148432546
1202	4	1.0911587129678963
1202	5	1.0373708142743325
1202	6	1.0107434893794647
1202	7	0.9886225845832044
1202	8	1.0237009006989837
1202	9	1.1229797291265626
1202	10	1.1668980197398753
1202	11	1.1352666125105486
1202	12	1.1839080763620373
1202	13	1.171961757277309
1202	14	1.4052765195120713
1202	15	1.31465258712052
1202	16	1.318783448998822
1202	17	1.275926117926545
1202	18	1.4039291701187153
1202	19	1.3541287458111881
1202	20	1.370680962793891
1202	21	1.3946197629397048
1202	22	1.4236875829564113
1202	23	1.3938299220025172
1202	24	1.3865683522609942
1202	25	1.4481098763514098
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1203	2	0.7746299904526452
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2211	5	-0.3722955399479986
2211	6	-0.4214176713885749
2211	7	-0.5242648670751606
2211	8	-0.3743059746901878
2211	9	-0.4338411083493341
2211	10	-0.48143130668135825
2211	11	-0.5060486551255607
2211	12	-0.5439375886391318
2211	13	-0.6277423383657563
2211	14	-0.7247140829528291
2211	15	-0.6438085649942877
2211	16	-0.6130705884618237
2211	17	-0.6937007823815193
2211	18	-0.7909779830816817
2211	19	-0.7799600049217688
2211	20	-0.7811443556058535
2211	21	-0.7107290865127496
2211	22	-0.6126495930994196
2211	23	-0.7306422396907593
2211	24	-0.5949733396947039
2211	25	-0.6823537858069031
2212	1	-0.6271197552064862
2212	2	-0.5640521030154204
2212	3	-0.5255873464983303
2212	4	-0.5474913896476252
2212	5	-0.6123805923374945
2212	6	-0.5390866609701561
2212	7	-0.5879565174682
2212	8	-0.539542446106438
2212	9	-0.7158105965480672
2212	10	-0.7081237798422748
2212	11	-0.7779228806829153
2212	12	-0.745668474155444
2212	13	-0.6875283195907897
2212	14	-0.8546345817682379
2212	15	-0.8694384780584556
2212	16	-0.9290455683882684
2212	17	-0.9512930463809681
2212	18	-0.884590727062097
2212	19	-0.9654479657461223
2212	20	-0.9375797881537551
2212	21	-0.8326251203750985
2212	22	-0.7450056830685909
2212	23	-0.8063387672138305
2212	24	-0.7836198369835401
2212	25	-0.9903134206169213
2213	1	-0.9924229914799838
2213	2	-0.8641053904678735
2213	3	-0.9023999057758267
2213	4	-0.8429644480483773
2213	5	-0.7943357741397108
2213	6	-0.8433004716977237
2213	7	-0.8673997134272469
2213	8	-0.8984847706814634
2213	9	-0.9485773194157474
2213	10	-1.101677723228768
2213	11	-0.9745939100201468
2213	12	-1.0116005790404898
2213	13	-1.1604536550525566
2213	14	-1.2406032288113238
2213	15	-1.2301421637866952
2213	16	-1.2741733701006766
2213	17	-1.2174894416956548
2213	18	-1.1719389057429765
2213	19	-1.2097822061162304
2213	20	-1.1875344746510237
2213	21	-1.2429733519600674
2213	22	-1.2114662921403365
2213	23	-1.216631564513726
2213	24	-1.2391828936744886
2213	25	-1.2501003321451805
2214	1	-1.0337493467063226
2214	2	-1.0629723667307651
2214	3	-1.0270692628379905
2214	4	-1.0549393051785287
2214	5	-1.12333251474859
2214	6	-1.031312640061003
2214	7	-1.0879355333293077
2214	8	-1.1296983599800328
2214	9	-1.2266952243368792
2214	10	-1.639611369742191
2214	11	-1.5186404884987326
2214	12	-1.3734341626207467
2214	13	-1.3541707502465314
2214	14	-1.5242960886704324
2214	15	-1.4941852241779188
2214	16	-1.4855219010723744
2214	17	-1.5899966550789983
2214	18	-1.549112688484179
2214	19	-1.5761336172242508
2214	20	-1.5097591239225494
2214	21	-1.5334738015879665
2214	22	-1.4683323749315278
2214	23	-1.6234638330642355
2214	24	-1.5984648431984425
2214	25	-1.5100586345308002
2215	1	-1.706122063529513
2215	2	-1.3480445739814897
2215	3	-1.4993893950166672
2215	4	-2.205081966236044
2215	5	-1.7348555918167463
2215	6	-1.5322791910171745
2215	7	-1.5376031679381015
2215	8	-1.6979272070084832
2215	9	-1.7358594898217419
2215	10	-1.862392378506513
2215	11	-1.8456936541699562
2215	12	-1.916684817991424
2215	13	-1.974008344738067
2215	14	-2.061319534494011
2215	15	-1.993289950064117
2215	16	-2.032566755157923
2215	17	-2.06960842503873
2215	18	-2.3794440567091826
2215	19	-2.74076803490113
2215	20	-2.4259706654490643
2215	21	-2.203942360461525
2215	22	-3.1775622218338384
2215	23	-2.4211361782562832
2215	24	-2.3388333521296616
2215	25	-2.2966294014459123
2216	1	-3.1061212308509347
2216	2	-5.955552257477827
2216	3	-3.4476962200575905
2216	4	-6.290177770884583
2216	5	-3.8976437291518735
2216	6	-2.819464910289909
2216	7	-2.6223521800600054
2216	8	-2.449935577962446
2216	9	-2.7918226405362887
2216	10	-2.755476557513944
2216	11	-2.5988318613459995
2216	12	-2.737336423373101
2216	13	-2.691463921562125
2216	14	-3.392150644560452
2216	15	-3.26011503081358
2216	16	-3.2964342339999675
2216	17	-3.0595444726085717
2216	18	-3.062539948426938
2216	19	-3.138900680779855
2216	20	-3.4842888303051183
2216	21	-3.620222493809571
2216	22	-3.5170117362147835
2216	23	-3.379465701934272
2216	24	-3.5118790215629616
2216	25	-3.726559906272687
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	146951.0
3	14.0
4	1034.0
5	6182.0
6	7642.0
7	6860.0
8	6288.0
9	5776.0
10	5556.0
11	5264.0
12	5201.0
13	4967.0
14	5158.0
15	5304.0
16	5462.0
17	5997.0
18	6688.0
19	8211.0
20	10427.0
21	13326.0
22	16599.0
23	20104.0
24	23566.0
25	27438.0
26	31533.0
27	35541.0
28	40722.0
29	48909.0
30	62379.0
31	80715.0
32	103536.0
33	138044.0
34	186922.0
35	262257.0
36	385678.0
37	675964.0
38	1857996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.048644348078355	18.6509160657402	13.023774606091616	25.27666498008983
2	14.922932999028093	20.838533216855943	40.529050331090765	23.7094834530252
3	18.396001172510307	25.433001437270697	29.82688925451038	26.34410813570861
4	12.137873545229223	17.476540683462115	38.446824445917564	31.938761325391095
5	13.77581166278711	37.23464245224244	34.55764185996475	14.431904025005704
6	30.719916394365132	37.93437808851415	18.417876851926287	12.927828665194427
7	27.209978165727165	31.66565492295101	22.50003724572697	18.624329665594857
8	25.312168076096942	35.16487544016124	21.006911110837876	18.516045372903946
9	26.72088238096465	15.185617144882304	19.94175873305739	38.15174174109565
10	15.242871607732573	28.301793566875833	32.93085428176355	23.524480543628044
11	34.88150058590037	22.2447550331159	24.448927362540395	18.424817018443328
12	23.547078835639045	24.548756692748803	31.099090987334733	20.80507348427742
13	28.722687366850547	21.224230186296925	26.102258782248473	23.950823664604055
14	22.51757687429118	21.837628746525063	26.58901281009598	29.05578156908778
15	23.840988977338696	29.36270029782349	23.39417098307762	23.402139741760198
16	23.66812334456777	27.28684322403599	26.215449613147268	22.82958381824897
17	22.417093797203876	27.11865889681617	26.937079155430744	23.52716815054921
18	22.597256047311063	27.041216516117927	28.432512474393235	21.929014962177774
19	24.812857214814024	25.722186106775023	26.333931070916684	23.131025607494273
20	24.457633098261173	26.666116504487054	26.310954501430107	22.56529589582167
21	25.710744284335075	25.699347122322997	25.29113009955019	23.298778493791737
22	24.376405999593732	26.316623363908935	26.229316301992572	23.077654334504757
23	23.793580184560295	25.852817574630777	26.143109774999306	24.210492465809622
24	23.763755200060913	26.595345465173274	26.441480649140694	23.19941868562512
25	23.96833534250411	26.034360367202957	26.658440810964066	23.33886347932887
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19602.0
1	19602.0
2	15438.5
3	11275.0
4	11275.0
5	11275.0
6	7586.5
7	3898.0
8	3898.0
9	3898.0
10	5650.0
11	7402.0
12	7402.0
13	7402.0
14	12676.0
15	17950.0
16	17950.0
17	17950.0
18	31606.5
19	45263.0
20	45263.0
21	45263.0
22	74086.5
23	102910.0
24	102910.0
25	102910.0
26	150514.5
27	198119.0
28	198119.0
29	198119.0
30	253772.0
31	309425.0
32	309425.0
33	309425.0
34	342830.0
35	376235.0
36	376235.0
37	376235.0
38	395921.0
39	415607.0
40	415607.0
41	415607.0
42	447876.0
43	480145.0
44	480145.0
45	480145.0
46	501033.0
47	521921.0
48	521921.0
49	521921.0
50	511076.0
51	500231.0
52	500231.0
53	500231.0
54	449150.5
55	398070.0
56	398070.0
57	398070.0
58	354158.5
59	310247.0
60	310247.0
61	310247.0
62	269778.0
63	229309.0
64	229309.0
65	229309.0
66	188321.5
67	147334.0
68	147334.0
69	147334.0
70	112072.5
71	76811.0
72	76811.0
73	76811.0
74	60173.0
75	43535.0
76	43535.0
77	43535.0
78	34674.5
79	25814.0
80	25814.0
81	25814.0
82	18628.0
83	11442.0
84	11442.0
85	11442.0
86	8431.0
87	5420.0
88	5420.0
89	5420.0
90	3672.0
91	1924.0
92	1924.0
93	1924.0
94	1083.0
95	242.0
96	242.0
97	242.0
98	161.0
99	80.0
100	80.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.39674091259799105
2	0.6888625938949973
3	0.7035097557374506
4	0.7120539334788817
5	0.7224055334348463
6	0.7236965493023703
7	0.7402919714539961
8	0.7295178572141146
9	0.7174292540909358
10	0.7102934572958945
11	0.723508765176185
12	0.7278278000784468
13	0.726278581037418
14	0.7360903016306
15	0.7322642000595746
16	0.726560257226696
17	0.7327101873592646
18	0.7358320984570952
19	0.7391183206653379
20	0.7313018064128749
21	0.729729114356073
22	0.7384141301921431
23	0.7282268413465907
24	0.7317477937125649
25	0.7339777302110153
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4260211.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.94201829004212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.54479657247609	52.19009019062945
2	6.949274307753994	8.192085067003115
3	1.7258902553104145	3.051823649853358
4	0.7549824717657798	1.780007626379192
5	0.42608806092264634	1.25572451400356
6	0.27176085477110623	0.9610879963461615
7	0.20689648304864447	0.8536427401599015
8	0.14893368728449624	0.7022761695940952
9	0.11298237295747164	0.5993468183980489
>10	0.7117351844081067	7.785821243608365
>50	0.0649860124288633	2.689650664460504
>100	0.06417833810262842	8.176632309092783
>500	0.011289937041725992	4.596194470815444
>1k	0.006045320780213123	6.422694032771761
>5k	1.2010571086516125E-4	0.4105978343800651
>10k+	4.0035236955053756E-5	0.3323246725042791
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	14083	0.33057048113344617	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	6175	0.14494587239927786	No Hit
GATATACACTGTTCTACAAATCCCG	5664	0.13295116133919188	No Hit
TTGTAGAACAGTGTATATCAATGAG	5561	0.13053344071455616	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4727	0.1109569455597387	No Hit
GTGTATATCAATGAGTTACAATGAA	4472	0.1049713265375823	No Hit
GTTCTACAGTGTGGTTTTTATCATT	4285	0.10058187258800091	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.347301577316241E-5
2	0.0	0.0	0.0	0.0	2.347301577316241E-5
3	0.0	2.347301577316241E-5	0.0	4.694603154632482E-5	2.347301577316241E-5
4	0.0	2.347301577316241E-5	0.0	4.694603154632482E-5	2.347301577316241E-5
5	0.0	2.347301577316241E-5	0.0	4.694603154632482E-5	2.347301577316241E-5
6	0.0	2.347301577316241E-5	0.0	4.694603154632482E-5	2.347301577316241E-5
7	0.0	2.347301577316241E-5	0.0	9.389206309264964E-5	2.347301577316241E-5
8	0.0	2.347301577316241E-5	0.0	9.389206309264964E-5	2.347301577316241E-5
9	0.0	2.347301577316241E-5	0.0	1.6431111041213686E-4	2.347301577316241E-5
10	0.0	2.347301577316241E-5	0.0	1.8778412618529927E-4	2.347301577316241E-5
11	0.0	2.347301577316241E-5	0.0	1.8778412618529927E-4	2.347301577316241E-5
12	0.0	2.347301577316241E-5	0.0	2.1125714195846168E-4	7.041904731948723E-5
13	0.0	2.347301577316241E-5	0.0	2.1125714195846168E-4	1.1736507886581205E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1580	0.0	15.462067	1
AATCGTA	125	7.2759576E-12	13.679541	13
GTATCAA	5535	0.0	13.653407	1
CCAACGA	735	0.0	11.373498	19
CGAACGA	230	0.0	11.151668	16
CGTCGTA	530	0.0	11.112703	10
TCCAACG	1055	0.0	11.075327	18
CGCATCG	385	0.0	10.856778	13
CTCGACG	105	3.4716104E-6	10.856778	13
GTCGATA	105	3.4721033E-6	10.85665	9
AACCGCG	370	0.0	10.783294	7
ACTGTTC	2675	0.0	10.760259	8
CGTTCAA	80	3.775151E-4	10.687015	14
GTATTAG	795	0.0	10.641783	1
CGACCAT	475	0.0	10.599519	10
CGTCTTA	280	0.0	10.517379	15
TGAGTTA	3530	0.0	10.441453	12
GAACAGT	1650	0.0	10.420739	6
GATATAC	2665	0.0	10.415429	1
CCGTCGT	570	0.0	10.332865	9
>>END_MODULE