FastQCFastQC Report
Thu 2 Feb 2017
SRR4050214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4260211
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80170.18818316745344302No Hit
GATATACACTGTTCTACAAATCCCG59390.13940624067681154No Hit
TTGTAGAACAGTGTATATCAATGAG58570.13748145338341222No Hit
TATCAACGCAGAGTACTTTTTTTTT51140.12004100266395255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.016.1401331
GTATCAA57300.013.21931
GGACCGT853.9645674E-612.2894756
CCAACGA7450.012.23368519
CGAACGA3000.012.025416
TCCAACG10150.011.97211718
CGTCGTA5250.011.93484610
CGTCTTA3900.011.9281715
ACGAACG3250.011.68473715
TCGCCAG4400.011.65140517
CGCATCG4500.011.60359313
CGCCAGT4500.011.39221818
ACGCATC2450.011.23735410
GTCTACG600.005698049411.129851
CGAACGT2400.011.0967384
ATAACGA3350.011.05241112
GATAACG3300.010.93218311
CGATAAC3300.010.93205510
ATTCCGA3650.010.9273796
ATCGCCA4800.010.87824116
Produced by FastQC (version 0.11.5)