##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050214_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4260211
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.847602618743533	31.0	31.0	34.0	27.0	34.0
2	30.860920973163065	31.0	31.0	34.0	27.0	34.0
3	31.085345068589326	31.0	31.0	34.0	27.0	34.0
4	34.69507566644	37.0	35.0	37.0	32.0	37.0
5	34.630029827161145	37.0	35.0	37.0	32.0	37.0
6	34.66773265455631	37.0	35.0	37.0	32.0	37.0
7	34.57658388281707	37.0	35.0	37.0	31.0	37.0
8	34.55664355591777	37.0	35.0	37.0	31.0	37.0
9	36.181354397704716	39.0	37.0	39.0	32.0	39.0
10	36.064985044168	39.0	37.0	39.0	32.0	39.0
11	36.070679363064414	39.0	37.0	39.0	32.0	39.0
12	35.97438178531533	39.0	37.0	39.0	31.0	39.0
13	35.985227022793005	39.0	37.0	39.0	31.0	39.0
14	37.15117913173784	40.0	37.0	41.0	31.0	41.0
15	37.13276361194316	40.0	37.0	41.0	31.0	41.0
16	37.10063116592112	40.0	37.0	41.0	31.0	41.0
17	37.09299398550917	40.0	37.0	41.0	31.0	41.0
18	37.07505590685532	40.0	37.0	41.0	31.0	41.0
19	37.12182941173571	40.0	37.0	41.0	31.0	41.0
20	37.09597529324252	40.0	37.0	41.0	31.0	41.0
21	37.0257952012236	40.0	37.0	41.0	31.0	41.0
22	36.967028158933914	40.0	37.0	41.0	31.0	41.0
23	36.88072187034867	40.0	37.0	41.0	31.0	41.0
24	36.81194006587937	40.0	37.0	41.0	31.0	41.0
25	36.53508359093012	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8632699207901204
1101	2	0.9782615663148135
1101	3	0.9472779650533774
1101	4	0.82876790828211
1101	5	0.9064896356500185
1101	6	0.8993755326002031
1101	7	0.9836669910315265
1101	8	0.9997028915194761
1101	9	1.081524855602936
1101	10	1.1211419253775716
1101	11	1.1234968939095396
1101	12	1.2206685743332528
1101	13	1.1950252342691527
1101	14	1.302924090872331
1101	15	1.3578520330674664
1101	16	1.4044074622542126
1101	17	1.2849301017611339
1101	18	1.3087251813638758
1101	19	1.3146521848724149
1101	20	1.3881617903500967
1101	21	1.3484494031305871
1101	22	1.4490613960559813
1101	23	1.4957595771131196
1101	24	1.489294802594081
1101	25	1.4813522392550524
1102	1	0.7938589139166368
1102	2	0.8261049524811916
1102	3	0.7772955041963883
1102	4	0.7221436643754942
1102	5	0.798348993689082
1102	6	0.8097247724471401
1102	7	0.8400383896282548
1102	8	0.8528693368412092
1102	9	1.0005446176284991
1102	10	1.0102396938029372
1102	11	0.9844646530161825
1102	12	1.056024333243144
1102	13	1.0500796810786497
1102	14	1.2376620380177243
1102	15	1.2414815373693742
1102	16	1.2296588420383117
1102	17	1.158743615458576
1102	18	1.2020773542837873
1102	19	1.2568201792717488
1102	20	1.2336502281939445
1102	21	1.2375532358005614
1102	22	1.3067918592497207
1102	23	1.318616590984746
1102	24	1.342206549642313
1102	25	1.2946594154799769
1103	1	0.690010068750265
1103	2	0.7926231030073794
1103	3	0.736427352477456
1103	4	0.6665358255671521
1103	5	0.6677459024703438
1103	6	0.7011530111101081
1103	7	0.7562305117166161
1103	8	0.7922018070331305
1103	9	0.9182012424805137
1103	10	0.9756549652317545
1103	11	0.9620373203710528
1103	12	1.011299583520632
1103	13	0.9406153495088674
1103	14	1.1083866347883315
1103	15	1.1743317352479679
1103	16	1.1755382499881577
1103	17	1.1933586888450876
1103	18	1.1542470261201032
1103	19	1.1334139544567776
1103	20	1.1355807548209071
1103	21	1.0942874694764555
1103	22	1.1280594937386468
1103	23	1.22830738023994
1103	24	1.2653977413902098
1103	25	1.1984358306709524
1104	1	0.6816690629385036
1104	2	0.6798531636664045
1104	3	0.7141016106249616
1104	4	0.5981850777513031
1104	5	0.6140786621326555
1104	6	0.5962590473628424
1104	7	0.6586465621699418
1104	8	0.7155987792093441
1104	9	0.7846543615937946
1104	10	0.8681696172504871
1104	11	0.8724570290918194
1104	12	0.9019917339621415
1104	13	0.8661181104864539
1104	14	1.0232293136834087
1104	15	1.0567281902285401
1104	16	1.009663409103844
1104	17	1.008913570659196
1104	18	1.0027500568419754
1104	19	1.058174695740803
1104	20	1.053093456209531
1104	21	1.0220850631791478
1104	22	0.9708892003861678
1104	23	1.071057835572745
1104	24	1.0668233176063424
1104	25	1.0633289472874239
1105	1	0.5311254161713066
1105	2	0.5022819402617813
1105	3	0.542486759204138
1105	4	0.4882920006709881
1105	5	0.4410729095390735
1105	6	0.4599483555462527
1105	7	0.6056131531811886
1105	8	0.549814800163972
1105	9	0.6753539743725696
1105	10	0.5859607713239754
1105	11	0.6976194892855716
1105	12	0.6578954182947143
1105	13	0.6964111594666917
1105	14	0.7734681379591279
1105	15	0.7293880794233729
1105	16	0.8014788380552744
1105	17	0.7653574948882778
1105	18	0.8313473293382785
1105	19	0.8380083453761102
1105	20	0.8272119614331359
1105	21	0.8396359404351443
1105	22	0.7819735201714266
1105	23	0.8559321220446137
1105	24	0.8847894633475164
1105	25	0.8174753201850038
1106	1	0.4641899167810095
1106	2	0.47433756146288886
1106	3	0.5212309409809635
1106	4	0.4255683177913028
1106	5	0.43948269443922783
1106	6	0.4044897735172768
1106	7	0.5307074348131167
1106	8	0.5366123318510816
1106	9	0.620273388115848
1106	10	0.5748105743546574
1106	11	0.6036624172047453
1106	12	0.6080356756785292
1106	13	0.6617217443180508
1106	14	0.7206717053388232
1106	15	0.7362131314938907
1106	16	0.6505813731894605
1106	17	0.676528764757613
1106	18	0.6886111916411011
1106	19	0.6628859640318865
1106	20	0.7283578518734544
1106	21	0.6954759499938206
1106	22	0.7180684937320692
1106	23	0.8191896065041036
1106	24	0.7538368903861183
1106	25	0.7619557654231244
1107	1	0.4494143786451055
1107	2	0.4381595623970185
1107	3	0.4706081133835234
1107	4	0.4224611828964342
1107	5	0.30580641654574237
1107	6	0.378315896408985
1107	7	0.35268074162421925
1107	8	0.35931429718350927
1107	9	0.4476349848709589
1107	10	0.42368699437000856
1107	11	0.41673301280355446
1107	12	0.5233890579747893
1107	13	0.46964554836315386
1107	14	0.5142984436940523
1107	15	0.601836448104514
1107	16	0.5944997674780907
1107	17	0.4818318480049868
1107	18	0.4760366315700608
1107	19	0.5504693417422004
1107	20	0.5693148333452456
1107	21	0.5216920237416289
1107	22	0.5308210863683058
1107	23	0.5306655955717687
1107	24	0.566617639785342
1107	25	0.4932690603823957
1108	1	0.32124217819924183
1108	2	0.29572323811913037
1108	3	0.30387549037518014
1108	4	0.25569328799068103
1108	5	0.23657191829889967
1108	6	0.2667675963318814
1108	7	0.3355724436517278
1108	8	0.3779653369250866
1108	9	0.4221982125206054
1108	10	0.3915195154432709
1108	11	0.4318442473077937
1108	12	0.44859435586527496
1108	13	0.46253171923286374
1108	14	0.5756294857349715
1108	15	0.5206262629848624
1108	16	0.539253139443403
1108	17	0.41578963201421004
1108	18	0.4600580173404225
1108	19	0.4991387369136575
1108	20	0.46232264662054234
1108	21	0.5266241912252383
1108	22	0.5221735733483612
1108	23	0.5984958416427588
1108	24	0.5540722427234854
1108	25	0.46327455970888565
1109	1	0.24164005105669162
1109	2	0.2954446047700081
1109	3	0.2566484498728734
1109	4	0.22244804903849058
1109	5	0.21547765889473425
1109	6	0.22285279958660453
1109	7	0.23934471070617747
1109	8	0.25233617497234917
1109	9	0.3408188362706355
1109	10	0.3150016757711498
1109	11	0.26587734264218454
1109	12	0.32725982610078574
1109	13	0.2600730310497994
1109	14	0.3829204535417574
1109	15	0.34922444697684796
1109	16	0.37085982154892605
1109	17	0.40192536052650496
1109	18	0.3609123262832199
1109	19	0.4559334642910784
1109	20	0.45549762273049765
1109	21	0.44722355065359665
1109	22	0.4521580985191065
1109	23	0.3850448687483592
1109	24	0.41945339972923534
1109	25	0.49035905151647086
1110	1	0.06723281798416281
1110	2	0.03970458211214378
1110	3	-0.052635256509329764
1110	4	0.027609735854269957
1110	5	-0.08519470641414273
1110	6	-0.03991084720435367
1110	7	-0.024717861289843768
1110	8	-0.05818403526327387
1110	9	-0.04541441410627556
1110	10	-0.04561014954053633
1110	11	-0.02995752731482071
1110	12	0.011777575358152603
1110	13	0.03074132079913028
1110	14	0.06475336206133875
1110	15	0.05779686025049102
1110	16	-0.08470960825189877
1110	17	-0.12901628926067588
1110	18	-0.09598935411571574
1110	19	-0.0825904987918662
1110	20	-0.06931266481834086
1110	21	-0.06524167659413393
1110	22	-0.11033113661381577
1110	23	-0.08071095201611911
1110	24	-0.12351807934522441
1110	25	-0.08201885640036721
1111	1	0.017340602036153285
1111	2	-0.036322028604672596
1111	3	0.0036498920283172254
1111	4	-0.07106663569419425
1111	5	-0.04832363858933775
1111	6	-0.0898275268223756
1111	7	-0.07822206616300775
1111	8	-0.1149706890361486
1111	9	-0.09646793486010097
1111	10	-0.05829816003976873
1111	11	-0.07982456007311356
1111	12	-0.0786423075616014
1111	13	-0.03686187271713237
1111	14	-0.11441423998094535
1111	15	-0.11575848808338662
1111	16	-0.1674946675691018
1111	17	-0.06518481804434373
1111	18	-0.0717444180954061
1111	19	-0.17362341250130697
1111	20	-0.1143819401756545
1111	21	-0.13627841814840735
1111	22	-0.1332449611776454
1111	23	-0.17758276814141993
1111	24	-0.07532321038414835
1111	25	-0.11883236264867492
1112	1	-0.2730186400016059
1112	2	-0.36333294765358204
1112	3	-0.3114951045080758
1112	4	-0.3083313363296938
1112	5	-0.32171421989605165
1112	6	-0.30540981817772916
1112	7	-0.31232260574427073
1112	8	-0.28341503154167214
1112	9	-0.31897662862862575
1112	10	-0.3166141753398719
1112	11	-0.3015948916758049
1112	12	-0.2838447837507516
1112	13	-0.2928994372108775
1112	14	-0.36016975800443873
1112	15	-0.3546622044522323
1112	16	-0.4201266611006176
1112	17	-0.36939049372565336
1112	18	-0.3634982541266467
1112	19	-0.31688073183193666
1112	20	-0.41544419010672584
1112	21	-0.34248093835664406
1112	22	-0.4167688507963234
1112	23	-0.3967440424706439
1112	24	-0.4845462504345619
1112	25	-0.4412950680614003
1113	1	-0.4039453865805527
1113	2	-0.4243448464174264
1113	3	-0.4239932104541744
1113	4	-0.4903766383669108
1113	5	-0.535151403365127
1113	6	-0.5754643967871189
1113	7	-0.6469387080761777
1113	8	-0.6072057294417519
1113	9	-0.6278482278221844
1113	10	-0.6661473237090476
1113	11	-0.6270147745019798
1113	12	-0.5779723209432532
1113	13	-0.6567117366557653
1113	14	-0.6987849452190744
1113	15	-0.6471365964702613
1113	16	-0.6847297607058351
1113	17	-0.69090425892567
1113	18	-0.7633548658489531
1113	19	-0.8484179795682181
1113	20	-0.7300842750797685
1113	21	-0.8492977783059175
1113	22	-0.7696120216403557
1113	23	-0.7939684231056887
1113	24	-0.868458175248648
1113	25	-0.8365230225802236
1114	1	-0.690456098556794
1114	2	-1.2534055920727205
1114	3	-0.8536828682742836
1114	4	-0.7494139051199227
1114	5	-0.8397274057581399
1114	6	-0.7744444000549535
1114	7	-0.7078901676655036
1114	8	-0.7852254994635004
1114	9	-0.8600597570112427
1114	10	-0.8421940762733655
1114	11	-0.8254656109105767
1114	12	-0.8566498059059526
1114	13	-0.828281904621619
1114	14	-1.010531674384552
1114	15	-1.0167510322833735
1114	16	-1.0220888718263268
1114	17	-1.0082959683113017
1114	18	-1.0312132238760157
1114	19	-1.0273869857432558
1114	20	-1.0668834151085704
1114	21	-1.0231026268520793
1114	22	-1.0928565351936754
1114	23	-1.2396182395721453
1114	24	-1.295843301662451
1114	25	-1.257880453932998
1115	1	-0.9466697038691727
1115	2	-1.1906833645704076
1115	3	-1.127560190956217
1115	4	-0.9714878078531726
1115	5	-1.1738894710800736
1115	6	-1.0962731602374376
1115	7	-1.2151403131585852
1115	8	-1.2892550853722327
1115	9	-1.547300856414175
1115	10	-1.3603846526496213
1115	11	-1.4541456842820182
1115	12	-1.3465813236130018
1115	13	-1.7520903807143142
1115	14	-1.6219253475678528
1115	15	-1.7873081549341805
1115	16	-1.7755818330628514
1115	17	-1.7046926451355802
1115	18	-1.630396045971196
1115	19	-1.5781871661600917
1115	20	-1.5779103934132834
1115	21	-1.5893064628920541
1115	22	-1.831109413450001
1115	23	-1.7761634699327402
1115	24	-1.732637858570854
1115	25	-1.7078840518097635
1116	1	-0.8353017812501378
1116	2	-0.7578572247730619
1116	3	-0.8812122860729801
1116	4	-0.9595749786884369
1116	5	-1.203453103458763
1116	6	-1.1858737327878046
1116	7	-1.1761151159682015
1116	8	-1.2507940854893391
1116	9	-1.3549776042745805
1116	10	-1.2625202632914068
1116	11	-1.4095295450134486
1116	12	-1.5702309197784245
1116	13	-1.443864797095621
1116	14	-1.6113980883420211
1116	15	-1.6908900502093829
1116	16	-1.7839195316073955
1116	17	-1.83005654142638
1116	18	-1.7777747142899898
1116	19	-1.7931378757905136
1116	20	-1.6972743333155549
1116	21	-1.6475067400130072
1116	22	-1.6796253101454255
1116	23	-1.8886216097111372
1116	24	-1.8144575451938536
1116	25	-2.0283344329259663
1201	1	0.6550961968269213
1201	2	0.7842610524501232
1201	3	0.7859782489149474
1201	4	0.7100741257513121
1201	5	0.7889918013440322
1201	6	0.8557699266855963
1201	7	0.8414458997442154
1201	8	0.8870011482574611
1201	9	0.9763898394322652
1201	10	1.0034513995376386
1201	11	1.0139163765191768
1201	12	1.004415649082027
1201	13	1.077860654965356
1201	14	1.2599798655851515
1201	15	1.2052247337508604
1201	16	1.19991805233186
1201	17	1.182831491343336
1201	18	1.2547578014202685
1201	19	1.2460625889483552
1201	20	1.2481865936686134
1201	21	1.205016110291524
1201	22	1.285603324618286
1201	23	1.3711239057881457
1201	24	1.3046783911525424
1201	25	1.3255053289089886
1202	1	0.7549946329266852
1202	2	0.8616718089729467
1202	3	0.7980116809460789
1202	4	0.7260038401523232
1202	5	0.841142277208931
1202	6	0.8122035053624828
1202	7	0.8784944121836844
1202	8	0.8502791939254877
1202	9	0.931469153707674
1202	10	1.040818168262362
1202	11	1.0446583800483396
1202	12	1.019574954379614
1202	13	0.9955112333429312
1202	14	1.1617068669803032
1202	15	1.1547669697903444
1202	16	1.2221888764522717
1202	17	1.2585676517238724
1202	18	1.2094302967567927
1202	19	1.2324345844114077
1202	20	1.2025671613561144
1202	21	1.2954666872459342
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2211	1	-0.33924447836784566
2211	2	-0.4538650819496901
2211	3	-0.41755949615419397
2211	4	-0.3213784955072967
2211	5	-0.3942821579556437
2211	6	-0.44459785930726525
2211	7	-0.4259843453326795
2211	8	-0.4541607874125688
2211	9	-0.5484951925403578
2211	10	-0.5293671864688676
2211	11	-0.5468481119964324
2211	12	-0.5024181460263648
2211	13	-0.4935984818203778
2211	14	-0.568726926386482
2211	15	-0.5676854419511841
2211	16	-0.5994917029635545
2211	17	-0.613426858614929
2211	18	-0.6245798487083363
2211	19	-0.5685434853845024
2211	20	-0.7323383096068312
2211	21	-0.723970973451614
2211	22	-0.6883749011105991
2211	23	-0.647399427555527
2211	24	-0.7060621760706312
2211	25	-0.6672517629320183
2212	1	-0.48483454171105933
2212	2	-0.5341118131821538
2212	3	-0.5018240110680985
2212	4	-0.4012573602858964
2212	5	-0.4714557024991066
2212	6	-0.5291171348750225
2212	7	-0.6003473941337205
2212	8	-0.6003417566926004
2212	9	-0.5494429616498877
2212	10	-0.5832453969983149
2212	11	-0.64344908940501
2212	12	-0.699035281406374
2212	13	-0.6459536796316172
2212	14	-0.7546501611528811
2212	15	-0.738254440454341
2212	16	-0.7310560523325975
2212	17	-0.7417580276392073
2212	18	-0.7680263539274605
2212	19	-0.8586407305990704
2212	20	-0.7763783972365701
2212	21	-0.7914981099220384
2212	22	-0.8028491722671376
2212	23	-0.7003534615963147
2212	24	-0.8224003634039718
2212	25	-0.686116244142184
2213	1	-0.749682074425337
2213	2	-0.7246075935059757
2213	3	-0.6569170264248534
2213	4	-0.6599263854015405
2213	5	-0.7231533103908632
2213	6	-0.6822282403634787
2213	7	-0.782940146886844
2213	8	-0.7015069117590826
2213	9	-0.9102649915261267
2213	10	-0.9793203735940068
2213	11	-0.9218633391598487
2213	12	-0.918900000282477
2213	13	-0.9556314477230927
2213	14	-1.1215810032403084
2213	15	-1.1308556501945262
2213	16	-1.1675446929045137
2213	17	-1.1130030448790365
2213	18	-1.1511987131508334
2213	19	-1.1142654703914516
2213	20	-1.1016146446651618
2213	21	-1.1297642895643918
2213	22	-1.0656814808712554
2213	23	-1.1497334103330132
2213	24	-1.1468844526907347
2213	25	-1.0580873125057693
2214	1	-0.8753789012625148
2214	2	-1.441394189298265
2214	3	-0.9673483142206187
2214	4	-0.8621325959031552
2214	5	-0.9060039160927715
2214	6	-0.9822669937629058
2214	7	-0.9591158566371476
2214	8	-1.050863930825372
2214	9	-1.2084391699671002
2214	10	-1.1938837241741354
2214	11	-1.1729472605139293
2214	12	-1.273776231542172
2214	13	-1.2237924395742183
2214	14	-1.5054112522362857
2214	15	-1.5784863907473792
2214	16	-1.6352478684036598
2214	17	-1.4765473579792854
2214	18	-1.4615998896573075
2214	19	-1.5437790250648789
2214	20	-1.4910192815315142
2214	21	-1.5663262676141159
2214	22	-1.5842708639368723
2214	23	-1.516871230781497
2214	24	-1.4616938425588941
2214	25	-1.4093444616687023
2215	1	-1.052339450921437
2215	2	-1.231656450544417
2215	3	-1.2003592958401725
2215	4	-0.9667239767085078
2215	5	-1.1716993167383691
2215	6	-1.1585646333080533
2215	7	-1.0530370922717225
2215	8	-1.078406912376586
2215	9	-1.3696400307045309
2215	10	-1.3618173801299491
2215	11	-1.4105979033359972
2215	12	-1.5963982259100504
2215	13	-1.5861932983322546
2215	14	-1.6936948108236791
2215	15	-1.8562283993892947
2215	16	-1.7244526199566153
2215	17	-1.7010020187088912
2215	18	-1.7509517699084824
2215	19	-1.7155765880018947
2215	20	-1.7004518015301642
2215	21	-1.5767626202602614
2215	22	-1.6050568493821658
2215	23	-1.6367455280851004
2215	24	-1.8140525280959068
2215	25	-1.7995493018275326
2216	1	-1.193419313757552
2216	2	-1.173382146127448
2216	3	-1.3210656136350316
2216	4	-1.1833767699242728
2216	5	-1.27369818882093
2216	6	-1.2474670461843047
2216	7	-1.3533050247063727
2216	8	-1.289744356185487
2216	9	-1.5539566201689254
2216	10	-1.585833133684602
2216	11	-1.6031733971890674
2216	12	-1.6469361317469549
2216	13	-1.5081778863568331
2216	14	-1.8193901766325027
2216	15	-1.8650575618929466
2216	16	-1.778870047702064
2216	17	-1.8561504748911304
2216	18	-2.007610080524927
2216	19	-1.9519755572544213
2216	20	-2.0237955744203404
2216	21	-1.8772803924721657
2216	22	-1.9938586258114057
2216	23	-1.956301246998521
2216	24	-2.08800331009423
2216	25	-2.0314446927796297
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51951.0
3	3.0
4	1008.0
5	7742.0
6	9321.0
7	7489.0
8	6160.0
9	5312.0
10	4839.0
11	4600.0
12	4277.0
13	4163.0
14	4249.0
15	4315.0
16	4418.0
17	4680.0
18	5366.0
19	6325.0
20	8078.0
21	11282.0
22	14406.0
23	18107.0
24	22154.0
25	25654.0
26	28744.0
27	32717.0
28	38554.0
29	47245.0
30	60826.0
31	80078.0
32	104586.0
33	139239.0
34	189755.0
35	261229.0
36	382589.0
37	661796.0
38	1996954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.536998394731114	19.29682197311098	13.516085812690653	25.65009381946725
2	15.145071884537794	21.421230762441358	39.251755740457924	24.18194161256293
3	18.206398697153734	25.54136403103039	30.161346468519984	26.090890803295892
4	12.478286303422736	18.05854200391363	37.62554037761644	31.837631315047194
5	13.677306013393192	37.54318507027405	34.47603995668209	14.303468959650672
6	30.707771134979268	38.26871868087485	18.13541133429453	12.88809884985136
7	27.048732797048004	31.97956889044334	22.334695563792057	18.6370027487166
8	25.408965445527098	35.35790916907442	20.73474936980673	18.498376015591752
9	26.671500556664135	15.165934736851494	19.838509891204613	38.32405481527976
10	15.183651758820393	28.589798240804033	32.804155180313835	23.422394820061747
11	34.90135922284518	22.27149012183829	24.400215485117325	18.426935170199208
12	23.526789633526583	24.695771337789235	30.99869534130275	20.77874368738143
13	28.81288772070752	21.244189837870625	26.0275075569373	23.91541488448455
14	22.507355989514984	21.778611829586126	26.61591147365342	29.098120707245467
15	23.81163756122237	29.474247579567077	23.347515366477428	23.366599492733126
16	23.693548519606544	27.39808247763888	26.064947883718663	22.843421119035913
17	22.443679119871003	27.24606864775192	26.811952616022694	23.498299616354387
18	22.647670022306514	27.017685357299708	28.474638813142416	21.860005807251365
19	24.852001558619587	25.730274307658362	26.392781525663235	23.024942608058815
20	24.455398945316308	26.60640908722795	26.41920449197133	22.518987475484415
21	25.63546655105011	25.621405712925572	25.451783649005797	23.291344087018512
22	24.30478305315899	26.28243874328221	26.297461706115637	23.11531649744316
23	23.777324737301075	25.797040213797544	26.23139570503544	24.194239343865938
24	23.73173224490869	26.582602951760308	26.48891974886986	23.196745054461136
25	23.999789667855968	26.000668086252144	26.760539905712044	23.239002340179848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	261.0
1	261.0
2	569.0
3	877.0
4	877.0
5	877.0
6	2346.5
7	3816.0
8	3816.0
9	3816.0
10	5642.5
11	7469.0
12	7469.0
13	7469.0
14	12722.0
15	17975.0
16	17975.0
17	17975.0
18	31899.0
19	45823.0
20	45823.0
21	45823.0
22	74831.0
23	103839.0
24	103839.0
25	103839.0
26	153296.0
27	202753.0
28	202753.0
29	202753.0
30	258726.0
31	314699.0
32	314699.0
33	314699.0
34	347279.5
35	379860.0
36	379860.0
37	379860.0
38	399752.0
39	419644.0
40	419644.0
41	419644.0
42	451164.5
43	482685.0
44	482685.0
45	482685.0
46	503467.0
47	524249.0
48	524249.0
49	524249.0
50	513557.5
51	502866.0
52	502866.0
53	502866.0
54	450892.5
55	398919.0
56	398919.0
57	398919.0
58	355474.0
59	312029.0
60	312029.0
61	312029.0
62	271182.0
63	230335.0
64	230335.0
65	230335.0
66	189241.0
67	148147.0
68	148147.0
69	148147.0
70	112044.5
71	75942.0
72	75942.0
73	75942.0
74	59377.5
75	42813.0
76	42813.0
77	42813.0
78	34592.5
79	26372.0
80	26372.0
81	26372.0
82	18928.0
83	11484.0
84	11484.0
85	11484.0
86	8360.0
87	5236.0
88	5236.0
89	5236.0
90	3560.0
91	1884.0
92	1884.0
93	1884.0
94	1031.5
95	179.0
96	179.0
97	179.0
98	117.0
99	55.0
100	55.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.43551833465525536
2	0.004342507918035046
3	0.0
4	0.005328374580507867
5	0.16442847549100267
6	0.03828448872602789
7	9.623936466996587E-4
8	2.3473015773162412E-4
9	2.816761892779489E-4
10	0.0019482603091724798
11	0.001314488883297095
12	0.002182990466904104
13	0.005117117438549405
14	0.009788247577408725
15	0.0029575999874184634
16	6.102984101022227E-4
17	0.006807174574217098
18	4.6946031546324824E-4
19	7.276634889680347E-4
20	0.0038965206183449595
21	0.0035678983975206864
22	0.0015492190410287192
23	0.0024881396719552153
24	0.0025585587192747026
25	0.0066193904480317985
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4260211.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.61719077297123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.22204311134632	51.7132833143908
2	7.156127191582809	8.389441455693127
3	1.7654935852556162	3.104648228861562
4	0.7696936850912252	1.8046912630297434
5	0.45468689754535796	1.3326234307693337
6	0.28622765872519285	1.0066716765597319
7	0.21581352958646724	0.8855267984610756
8	0.15061970057030802	0.7063122977998039
9	0.11471952574551762	0.6052082693408812
>10	0.7138679191783349	7.735286577165716
>50	0.065806682553016	2.704987825277896
>100	0.06707185624474744	8.45286805925963
>500	0.011363760921534814	4.6126943328495615
>1k	0.006304277128305474	6.3590367871153255
>5k	1.6061852556192255E-4	0.5867196834258327
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8017	0.18818316745344302	No Hit
GATATACACTGTTCTACAAATCCCG	5939	0.13940624067681154	No Hit
TTGTAGAACAGTGTATATCAATGAG	5857	0.13748145338341222	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5114	0.12004100266395255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.694603154632482E-5
6	0.0	0.0	0.0	0.0	4.694603154632482E-5
7	0.0	0.0	0.0	0.0	4.694603154632482E-5
8	0.0	0.0	0.0	0.0	4.694603154632482E-5
9	0.0	0.0	0.0	7.041904731948723E-5	4.694603154632482E-5
10	0.0	0.0	0.0	1.6431111041213686E-4	4.694603154632482E-5
11	0.0	0.0	0.0	1.6431111041213686E-4	4.694603154632482E-5
12	0.0	0.0	0.0	1.8778412618529927E-4	7.041904731948723E-5
13	0.0	0.0	0.0	1.8778412618529927E-4	9.389206309264964E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1720	0.0	16.140133	1
GTATCAA	5730	0.0	13.2193	1
GGACCGT	85	3.9645674E-6	12.289475	6
CCAACGA	745	0.0	12.233685	19
CGAACGA	300	0.0	12.0254	16
TCCAACG	1015	0.0	11.972117	18
CGTCGTA	525	0.0	11.934846	10
CGTCTTA	390	0.0	11.92817	15
ACGAACG	325	0.0	11.684737	15
TCGCCAG	440	0.0	11.651405	17
CGCATCG	450	0.0	11.603593	13
CGCCAGT	450	0.0	11.392218	18
ACGCATC	245	0.0	11.237354	10
GTCTACG	60	0.0056980494	11.12985	1
CGAACGT	240	0.0	11.096738	4
ATAACGA	335	0.0	11.052411	12
GATAACG	330	0.0	10.932183	11
CGATAAC	330	0.0	10.932055	10
ATTCCGA	365	0.0	10.927379	6
ATCGCCA	480	0.0	10.878241	16
>>END_MODULE