FastQCFastQC Report
Fri 10 Feb 2017
SRR3560103.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560103.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519903
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382452.5162789993835135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175441.1542841878725154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144740.9522976137292973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56360.370813137417322No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27880.18343275853788038No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27280.1794851381963191No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27160.17869561412800686No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20800.13685083850745738No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC20740.13645607647330124No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT19540.1285608357901787No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18870.12415265974210198No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17640.11606003804190135No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17580.11566527600774523No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16260.10698051125631043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4050.065.1941451
TACAACG3200.061.3541832
TAACGCA4450.056.1525544
GTATCAA183450.055.7067571
GTATAAC5400.047.7843481
ACAACGC4450.046.7937973
TATCACG2900.045.1341132
TATCAAC229600.044.673052
ATCAACG233250.044.076013
TCAACGC241200.043.2399144
AAACGCA6650.042.942325
AACGCAG253400.042.847176
CAACGCA244750.042.7328765
GACCGTT3500.042.4950077
GGTTTCA8600.041.8662151
AGATTAC8850.041.0078282
ACGCATA1800.040.0054931
ATAACGC6800.039.3716663
GTACAAC5650.039.2974361
ACGCAGA278250.039.041957