FastQCFastQC Report
Fri 10 Feb 2017
SRR3560101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850988
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440682.380782587461399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201711.0897423430081665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147680.7978441783523178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67390.3640758340950887No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA64520.3485706012140543No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT43190.23333484603898025No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41870.22620351941773797No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33770.1824431060601149No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32100.1734208973802099No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC29540.159590445751134No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28780.15548453042375207No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG22870.12355563623319006No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.11653236001529993No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21210.11458745275496113No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20200.10913090738567727No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20160.10891480657897297No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG19050.10291800919292832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6350.060.4351351
TATCACG3450.051.7374532
TACAACG4500.047.5984542
AAACGCA6550.047.231285
GTATCAA252100.044.2646261
ACAACGC5250.043.0652663
TAACGCA5600.042.498624
TCACGCA4400.040.5668644
TAGTACT9700.039.8666085
ATAGTAC9750.039.055144
GTATAAC6350.038.716261
GTATCAC6400.037.4768641
GTACAAC6650.036.969661
TATCAAC305500.036.5557672
AGATTAC10150.036.3436472
AACGCAG325350.036.2975546
GGTTTCA10250.036.2702941
ATCAACG307750.036.2305033
TCAACGC314350.036.1322754
CAACGCA317400.035.9508675
Produced by FastQC (version 0.11.5)