FastQCFastQC Report
Fri 10 Feb 2017
SRR3560096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5134519
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1346612.622660467319334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT602931.1742677356924767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422830.8235045970226227No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183510.3574044618395608No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA110760.2157164088788064No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101710.1980906098506988No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT82840.1613393581755175No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80650.1570741095709257No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC75340.14673234240636757No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT70110.13654638341001368No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66410.1293402556305664No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63630.12392592178546813No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG53870.10491732526454767No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC51820.10092474095431334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9600.058.7297061
GTATCAA590400.056.1696131
TATCAAC745300.044.483062
ATCAACG761150.043.431683
TCAACGC775600.043.2362334
CAACGCA782450.042.9895745
AACGCAG801700.042.825566
TATCACG9200.040.7443472
TAACGCA8950.039.2232554
ACGCAGA882450.038.9134837
CGCAGAG893050.038.5315448
TACAACG12350.037.578752
GACCGTT10200.037.3287737
GCAGAGT927600.037.0514769
ACAACGC12600.036.3609163
GTATCAC13950.033.9667551
AGATTAC20900.033.877812
GGTATCA315300.032.966721
CATGGGT73300.032.8749964
TAGTACT17400.032.8235745