FastQCFastQC Report
Fri 10 Feb 2017
SRR3560093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719567
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT641092.357323794559943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295391.0861655550313707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218490.803399953007225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94470.34737147494435694No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61470.2260286288221618No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50520.18576486624525154No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45000.1654675174393571No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40390.14851628954168072No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36690.13491118255222245No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT30170.11093677780323118No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG29880.1098704315797331No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC29380.10803190360818468No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28940.10641399899322208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG5250.062.332732
CGCATAG8200.059.9691541
GTATCAA304150.053.0583531
ACAACGC6900.047.4270743
TATCACG4300.047.046052
TATCAAC385450.041.7553332
TAGTACT11850.041.6708685
ATCAACG391850.040.9670643
TAACGCA6600.040.5677874
TCAACGC400800.040.52734
ATAGTAC11950.040.3301544
AACGCAG418000.040.06596
CAACGCA407400.040.0569275
GTACAAC10200.037.6277051
AGATTAC13900.036.8125882
ACGCAGA459100.036.3754127
CGCAGAG463000.036.0561648
GCAGAGT480800.034.659439
GCATAGT14400.033.4684222
GGTTTCA14900.033.4056361
Produced by FastQC (version 0.11.5)