FastQCFastQC Report
Fri 10 Feb 2017
SRR3560091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3818344
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1022642.678229096173629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452691.1855663083263321No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327760.8583825867967894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140340.36754153109306026No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA84190.22048825354656362No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70320.1841636060030212No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62310.16318592562639722No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62250.16302878944380075No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC57150.14967221392310384No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT54500.14273203252509464No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG52780.13822746195733018No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48150.12610178653363865No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47270.1237971225222243No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC44140.11559985166344362No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42480.11125241727827562No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40390.10577884025116646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8450.056.7940521
GTATCAA461250.055.2221341
TATCAAC579650.043.943142
ATCAACG588550.043.258413
TCAACGC604600.042.8186154
AACGCAG622550.042.7742086
CAACGCA608200.042.736785
TATCACG6850.042.5615232
TAACGCA9100.040.5379644
AGATTAC18900.039.980992
ACGCAGA685300.038.9530537
CGCAGAG694000.038.670488
ACAACGC9550.038.0047723
ATAGTAC13000.037.9884
TCACGCA8150.037.2326744
GCAGAGT721000.037.1563349
TAGTACT13350.036.9881825
GTATAAC11550.036.35681
GCATAGT14400.034.7079122
TACAACG10300.034.659772