FastQCFastQC Report
Fri 10 Feb 2017
SRR3560087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12714943
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4498043.5376013875956818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2064811.623923913776098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1528891.2024355909420907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509470.40068602745604126No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295010.2320183425124281No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT281700.22155034434680515No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC206700.16256462966448218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC176060.13846699902626383No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171640.13499077424098557No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT156870.12337452082954678No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT152730.12011850937908254No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151480.11913541413437717No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134490.1057731835683416No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC132720.10438112070183876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA401700.094.792841
GTATCAA1391000.085.522931
TATCAAC1927300.061.7437552
ATCAACG2017050.058.972823
TCAACGC2056950.057.8925254
CAACGCA2085450.057.120525
AACGCAG2123950.056.1887556
ACGCAGA2376000.050.1730777
CGCAGAG2399050.049.6934978
GCAGAGT2499300.047.635969
GACCGTT33350.042.8132487
GTACTGG127500.037.5963331
TACTGGT121300.037.082872
AGAGTAC2474700.036.5598910-11
GGACCGT53850.034.358766
GGTTCAC132000.033.442046
AGTACTT1817650.032.70482312-13
AGTACAT955700.032.38310212-13
ACTGGTT137950.032.0464133
TGGTTCA139700.031.8117085
Produced by FastQC (version 0.11.5)