FastQCFastQC Report
Fri 10 Feb 2017
SRR3560086.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560086.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510913
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT873833.4801285428846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400711.5958736921589876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295021.1749511034432496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128770.5128413449609763No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA101110.40268221160988055No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70440.2805354068420531No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68340.27217191515596123No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61510.24497065410071955No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG55970.22290696650979144No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49970.19901127597810037No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43500.17324375635476022No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC42070.1675486167780405No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34980.1393118757997589No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG34810.1386348312346943No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG34180.13612578372886674No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32350.12883759811670098No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30800.12266454472934746No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC27190.10828730425944666No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC26690.10629599671513906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7500.075.1946261
TATCACG4000.056.5248572
TACAACG4950.056.494812
AGATTAC13850.050.6930162
TAACGCA6600.050.4847224
GTATCAA440950.047.865951
ATAGTAC12050.047.402374
TAGTACT12200.046.8139655
ACAACGC6200.044.1450463
GCATAGT13100.043.6029432
GATTACT16800.041.7915653
AAACGCA8000.041.6449175
TCACGCA5400.040.7684144
GTATCAC7250.040.548831
GGTTTCA15950.039.495611
TATCAAC538900.039.129242
AACGCAG568550.038.507316
ATCAACG548000.038.4686053
GTATAAC8750.038.3972551
CAACGCA559650.038.311775
Produced by FastQC (version 0.11.5)