FastQCFastQC Report
Fri 10 Feb 2017
SRR3560082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335772
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT998302.992710532974076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT467071.4001856241973372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339821.0187147083193935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146650.4396283678860546No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA123470.3701392061567757No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT100570.30148943033276854No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74400.22303682625790971No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67180.2013926611291179No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG61640.18478481143195638No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53590.16065246665539493No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC52360.1569651642858085No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52150.1563356248568547No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC47240.14161639344655452No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA45950.13774922266869558No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42990.12887571452725186No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT42660.12788643828175308No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT41490.12437900432043918No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG39930.11970242570535396No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC36260.10870047473268557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8150.053.735711
TATCACG6050.052.1245462
TAACGCA6950.049.6552244
GTATCAA510100.047.4319151
GTATAAC8650.043.694041
TCACGCA8100.039.6671074
TACAACG5850.039.6671072
TATCAAC623000.038.804362
ATCAACG635000.038.0054633
TCAACGC649750.037.6646734
AACGCAG665950.037.592166
CAACGCA654650.037.4867675
ACGCAGA721300.034.7322167
CGCAGAG729850.034.472068
ATCACGC9100.034.0003743
GTATCAC11850.033.9198721
AGATTAC15150.033.383212
GCAGAGT757350.033.157519
ACAACGC7200.033.0559233
ATAGTAC13900.032.9607964
Produced by FastQC (version 0.11.5)