FastQCFastQC Report
Fri 10 Feb 2017
SRR3560081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3795748
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1241823.2716081257238363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT568841.4986242500819338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388781.02425134650667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163400.4304816863500949No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA101460.2672990936173845No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89250.23513152084911854No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT81420.21450317565865806No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71230.1876573471157727No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC65760.1732464852777371No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62550.16478965410770158No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58550.15425154673071026No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG53570.14113160304635608No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT53150.140025101771772No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC46130.12153072332515226No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA38530.10150831930886876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8900.061.354951
GTATCAA519150.058.1860121
TACAACG6750.056.4145852
TATCAAC659700.045.8627052
ATCAACG674150.044.8355333
TCAACGC690750.044.343794
CAACGCA696300.044.0711485
AACGCAG709650.043.9043736
ACAACGC8600.043.5870323
TCACGCA8200.042.81084
TATCACG7850.041.687732
ATAGTAC13700.040.3903434
ACGCAGA780150.039.9521067
CGCAGAG787250.039.644698
TAGTACT14000.038.670765
GCAGAGT821800.037.9489979
TAACGCA7450.037.536764
GGTATCA267200.034.876531
GTATAAC9650.034.822421
GTACAAC12450.034.220571
Produced by FastQC (version 0.11.5)