FastQCFastQC Report
Fri 10 Feb 2017
SRR3560080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3636955
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT986012.7110866095401236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448761.2338893387462864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339150.93251085042295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137260.3774036247355274No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA93980.2584029772158303No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT68080.18718955829808176No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67820.18647467455605032No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59510.16362589033958352No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG50370.13849497725432403No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49310.13558045122911885No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46860.1288440467368994No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC43330.11913812516239546No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39420.10838737350338401No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT39130.10759000317573356No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC36550.10049615681249836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6900.066.935681
GTATCAA461250.052.302411
TAACGCA7750.049.1338844
TATCACG7250.043.4951322
TATCAAC568650.042.4590072
ATCAACG578000.041.7412833
TCAACGC591350.041.382524
CAACGCA593650.041.248855
AACGCAG606400.041.1762476
TACAACG7700.040.180512
GTATAAC10900.039.0704041
ACGCAGA663450.037.5906687
CGCAGAG670150.037.3302578
ATAACGC10700.037.25583
TAGTACT12550.036.9758225
ACAACGC8850.036.3039053
GCAGAGT698050.035.7870869
AGATTAC17150.035.0396772
ATAGTAC13800.034.4916274
GTATCAC12000.034.489261