FastQCFastQC Report
Fri 10 Feb 2017
SRR3560076.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560076.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4742535
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1101492.3225764280073844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT504421.063608386653973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371990.7843695407624826No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA177690.37467303878621877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149190.314578595624492No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT140330.29589660382053057No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG88650.18692534688726598No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78090.1646587742631314No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC75810.15985121881019326No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA65550.13821721927197164No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC65430.1379641900376065No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG62360.1314908587917643No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56790.11974608516331456No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG56710.11957739900707112No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT55500.11702602089388903No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT52610.11093223349959462No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51280.10812782615204737No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC49510.10439564494516118No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48160.10154906605855307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8500.052.9109341
GTATCAA681100.045.9581681
TATCACG7700.040.1803672
TATCAAC833050.037.5820962
ATCAACG846800.036.9437523
TACAACG9200.036.8627972
CAACGCA866200.036.8348435
TCAACGC861400.036.8287124
AACGCAG882950.036.7222756
ACGCAGA957300.033.8080447
TCACGCA9350.033.726054
TAACGCA11300.033.6978954
CGCAGAG964650.033.599798
GCAGAGT997350.032.426579
TAGTACT16100.030.6705425
AGATTAC18750.030.4628962
CATGGGT69200.029.2330474
ACAACGC11450.029.0993843
ATAGTAC16200.029.0143814
AGTACTT555600.028.67623512-13
Produced by FastQC (version 0.11.5)