FastQCFastQC Report
Fri 10 Feb 2017
SRR3560073.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560073.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4800477
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1082852.255713338486988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449960.9373235201418526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374350.7798183388859066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129480.2697231962573719No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66220.13794462508621538No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA55540.11569683596025979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA442150.066.33211
TATCAAC571300.051.3149532
ATCAACG582200.050.3026053
TCAACGC592950.049.651544
GGTATCA197400.049.2312931
CAACGCA598200.049.220095
AACGCAG612950.048.5597846
ACGCAGA677750.043.890637
CGCAGAG689700.043.1905448
GCAGAGT725050.041.01059
CGCATAG7650.038.4244841
TATCACG6700.035.5230942
ACAACGC9700.031.8972073
TACAACG9950.031.6940982
AGAGTAC712200.031.3803110-11
AGTACTT486350.029.73800812-13
GAGTACT455250.028.83565512-13
GGACCGT13000.028.8315856
CATGGGG162450.028.2394184
TAACGCA7600.028.1844794
Produced by FastQC (version 0.11.5)