FastQCFastQC Report
Fri 10 Feb 2017
SRR3560072.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560072.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5581121
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1382882.4777817932992314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT631041.1306689104214012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441210.790540108340242No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA193870.34736749122622496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181580.3253468254854177No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT145120.26001944770593577No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99490.17826167897094508No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC87620.15699355022046646No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG86090.15425216546998355No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT84900.15211997733071905No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80160.14362705986843863No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC66820.11972505165180973No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG64450.11547859292066953No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64100.11485147876206232No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT63290.11340015742357136No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC63160.11316722930751724No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA61880.11087378324175377No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60420.10825782132299228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA721650.049.4985431
CGCATAG11100.045.949321
TATCAAC889500.040.2177732
ATCAACG905250.039.4983253
TCAACGC920050.039.3932844
TACAACG9850.039.2664952
CAACGCA927800.039.12175
AACGCAG944900.039.0307466
ACGCAGA1031850.035.7071847
CGCAGAG1042150.035.4513248
GCAGAGT1076550.034.2463659
TATCACG9950.033.48962
TACTGGT41400.031.7641282
TAACGCA9200.031.6922654
GTACTGG46300.031.6221941
AGATTAC21600.030.5783652
AGTACTT651250.029.26953912-13
CATGGGG169650.029.006594
ACAACGC14200.028.0757583
TAGTACT19600.027.6220845
Produced by FastQC (version 0.11.5)