FastQCFastQC Report
Fri 10 Feb 2017
SRR3560070.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560070.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3794336
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1085082.8597361962672783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489961.2912931274404797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350980.9250103311883818No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151190.3984623396557395No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA113400.2988665210461066No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT86240.22728614440049588No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77540.20435723141018614No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68890.18156009378188964No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG62170.16384948512730554No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53610.14128954315063294No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51570.13591310838049134No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44960.11849240552233645No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC44020.11601502871648689No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT38760.1021522606326904No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG38140.1005182461437258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9050.065.634211
TACAACG7600.056.365452
GTATCAA518150.052.617051
TATCACG7150.044.102572
TAACGCA9650.043.7748954
ACAACGC9750.043.325923
TATCAAC641450.042.5925032
GTATAAC11800.041.6941871
ATCAACG655050.041.6900373
TCAACGC669300.041.300234
AACGCAG689650.041.140526
CAACGCA674700.041.1086045
ATAGTAC15450.038.5092964
ACGCAGA751500.037.7308167
CGCAGAG760600.037.4201938
GCATAGT15950.037.302112
TAGTACT15950.037.3001445
AGATTAC16950.036.856462
GCAGAGT792100.035.8494579
TCACGCA9550.034.8882144