FastQCFastQC Report
Fri 10 Feb 2017
SRR3560065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867586
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT741781.9179405448256355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341820.8838071086202092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245930.6358746773827395No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA111770.28899163457515875No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101400.2621790439824738No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT79650.2059424147258781No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC55020.1422592800780642No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54010.13964783200683836No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52880.13672611287764513No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG52540.13584701154673742No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT47660.12322932185606217No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT44240.11438659670399057No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA43880.11345578353008827No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC43200.11169758086827287No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG41640.10766405711469636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7050.044.2539751
GTATCAA440350.044.1589851
TACAACG6700.040.8508452
TAACGCA6700.037.2985954
TATCAAC539500.035.8875732
ATCAACG539850.035.8532833
TCAACGC550950.035.832924
AACGCAG566650.035.801976
CAACGCA553250.035.7980885
ACAACGC7400.034.574413
ACGCAGA618100.032.8796047
CGCAGAG624800.032.5460668
GCAGAGT649550.031.2418429
TATCACG5350.028.9159562
CATGGGG112200.028.5834034
CATGGGT51100.027.8288334
AGTACTT366150.027.50099212-13
GTACTGG32100.027.4758011
AAACGCA12050.027.1545815
ATAGTAC11850.026.6118774