FastQCFastQC Report
Fri 10 Feb 2017
SRR3560063.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560063.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3053094
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAA333221.0914174276979354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320791.050704629467681No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCA310021.0154289386438806No Hit
GCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGT214580.7028280164318557No Hit
CTCCCAGTCCGTCCCCCGGCCGGCACGCGGCAACCCGCTCTCGCCGCGGG204220.6688952256301313No Hit
CCCCTTGTCCGTACCAGTTCTGAGGCGGCTGTTCGACGCCCGGGGAAAGT177170.5802965778321926No Hit
GTTCTGAGGCGGCTGTTCGACGCCCGGGGAAAGTCCCCGAAGGAACGCTC173870.5694878703374348No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAG153320.5021791009382613No Hit
GACTGGGAGCGTTCCTTCGGGGACTTTCCCCGGGCGTCGAACAGCCGCCT152230.49860895209908374No Hit
GTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTTGCCGCGTG148350.4859005323779746No Hit
GTCCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCT147190.4821011079252719No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA144770.4741747224291161No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA140350.4596976051179557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136170.4460065756245959No Hit
GTCCGTACCAGTTCTGAGGCGGCTGTTCGACGCCCGGGGAAAGTCCCCGA134150.43939033649144116No Hit
GTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCGCGGCG125190.41004305796022No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA111410.36490851575483757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105520.34561661055964865No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACC104640.3427342885610466No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGA97560.31954469793592993No Hit
TACCAGTTCTGAGGCGGCTGTTCGACGCCCGGGGAAAGTCCCCGAAGGAA91910.30103888055854156No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGGCGG91210.29874612442328996No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGGCGGCTGT86660.28384320954415426No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGC85330.2794869728871761No Hit
TCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTTGCCGCGTGC81010.2653373921667659No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG80820.26471507264434047No Hit
CCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAA79970.26193101162296345No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATC78810.2581315871702607No Hit
GAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAA77150.2526944797638068No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG76890.2518428846278562No Hit
GTACTGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAAT75390.24692983576660266No Hit
GTCGGATTCCCCTTGTCCGTACCAGTTCTGAGGCGGCTGTTCGACGCCCG71250.23336982090954292No Hit
GGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGG69410.22734314763973856No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAA68690.22498488418633686No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCC68420.22410053539131122No Hit
ACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTT66930.21922024018913275No Hit
GCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCG64680.2118506668972524No Hit
GAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGC64600.21158863762465221No Hit
GGGGACGGACTGGGAGCGTTCCTTCGGGGACTTTCCCCGGGCGTCGAACA63820.20903385221680038No Hit
GGTTAAAATTCCTGAACCGGGACGTGGTGGTTGACGGCAACGTTAGGGAG62820.20575848630929802No Hit
GAGTACGACCGGGCGTGAATGGCACTCGGTCCTCCGGATTTTCAAGGGTC61320.2008454374480445No Hit
GGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCG60940.19960079840319359No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT60090.1968167373818166No Hit
GTCCGTCCCCCGGCCGGCACGCGGCAACCCGCTCTCGCCGCGGGAGCAGC55190.18076744443505507No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGAT54670.17906425416315383No Hit
GCTCGAGCTGCTCCCGCGGCGAGAGCGGGTTGCCGCGTGCCGGCCGGGGG54520.1785729492770285No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGG52020.1703845345082726No Hit
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACC51010.1670764149416952No Hit
GACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCC49990.1637355417160428No Hit
TCCCAGTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCG48920.1602309001950153No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47560.15577640256081207No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGG46560.15250103665330972No Hit
CCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCC45610.1493894390411825No Hit
GTTTAACAGCCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCA44910.14709668290593084No Hit
ATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGG42040.13769638275139906No Hit
GTTATGAGTACGACCGGGCGTGAATGGCACTCGGTCCTCCGGATTTTCAA41420.13566565588874763No Hit
GATTCCCCTTGTCCGTACCAGTTCTGAGGCGGCTGTTCGACGCCCGGGGA41310.13530536563892234No Hit
ATTCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAG40170.13157144850436966No Hit
GTTCGACGCCCGGGGAAAGTCCCCGAAGGAACGCTCCCAGTCCGTCCCCC40060.1312111582545444No Hit
GAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTTCCCTTG39850.1305233314139689No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39280.12865637284669257No Hit
ATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAAT39060.12793579234704205No Hit
GGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACC38630.12652738500681604No Hit
AAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA38090.12475868741676477No Hit
GGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGTGGACT37370.12240042396336308No Hit
CTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAAC36920.12092650930498701No Hit
GCTCCCGCGGCGAGAGCGGGTTGCCGCGTGCCGGCCGGGGGACGGACTGG36440.11935433366938589No Hit
TCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAG36380.11915781171493572No Hit
CCCGGGGAAAGTCCCCGAAGGAACGCTCCCAGTCCGTCCCCCGGCCGGCA35990.11788041901100982No Hit
CCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCAT35980.11784766535193479No Hit
GGATTCCCCTTGTCCGTACCAGTTCTGAGGCGGCTGTTCGACGCCCGGGG35590.11657027264800887No Hit
CCTTCGGGGACTTTCCCCGGGCGTCGAACAGCCGCCTCAGAACTGGTACG34680.11358968967218172No Hit
CCCCGAAGGAACGCTCCCAGTCCGTCCCCCGGCCGGCACGCGGCAACCCG34620.11339316771773159No Hit
GGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGC34560.11319664576328145No Hit
GAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTCAGCC34490.11296737014975629No Hit
GTCCCCGAAGGAACGCTCCCAGTCCGTCCCCCGGCCGGCACGCGGCAACC34470.11290186283160622No Hit
TCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCC33770.11060910669635458No Hit
CCGGAGGACCGAGTGCCATTCACGCCCGGTCGTACTCATAACCGCATCAG33180.10867664081092819No Hit
CCCTAACGTTGCCGTCAACCACCACGTCCCGGTTCAGGAATTTTAACCCG32850.1075957700614524No Hit
GTACATGGGGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCA32730.10720272615255214No Hit
TTCCCCGGGCGTCGAACAGCCGCCTCAGAACTGGTACGGACAAGGGGAAT32720.10716997249347711No Hit
TGTCCGTACCAGTTCTGAGGCGGCTGTTCGACGCCCGGGGAAAGTCCCCG32100.10513924563082565No Hit
GAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATT31870.10438591147210009No Hit
GGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCC31280.1024534455866737No Hit
GGGAAAGTCCCCGAAGGAACGCTCCCAGTCCGTCCCCCGGCCGGCACGCG30690.10052097970124733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA228800.066.771921
TATCACG2650.065.1074752
GTACTGG17850.062.502261
CGCATAG3750.060.7817651
ATCACGC3050.056.5687873
TACAACG3250.054.9182432
TACTGGG20200.054.1932452
ACTGGGC19800.051.381843
TATCAAC297000.051.3818362
GGTATCA101200.051.0913931
ATCAACG308200.049.4374053
TCAACGC317350.048.5369264
CAACGCA319900.048.2794235
TCACGCA3600.047.9263344
AACGCAG330600.047.760596
ACAACGC3850.046.359553
GTATAAC4550.046.1400031
ACGCAGA372050.042.5355387
CGCAGAG374450.042.342358
AAACGCA5700.041.7500275
Produced by FastQC (version 0.11.5)