FastQCFastQC Report
Fri 10 Feb 2017
SRR3560061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5017565
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1681963.3521439184146096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT756021.5067467984968803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT542591.0813811081670093No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209030.4165964965077682No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116110.23140706697372132No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81170.16177169603184013No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77600.15465669104436117No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73260.14600707713801417No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC69670.1388522121786165No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT64840.12922602896026258No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62500.12456241224578057No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT53330.10628661512107965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA637750.063.8889271
CGCATAG8550.056.840941
TATCAAC822500.049.5093572
ATCAACG838850.048.4876253
TCAACGC859450.047.920814
CAACGCA869600.047.4872175
AACGCAG885050.047.2632456
ACGCAGA977450.042.7223327
CGCAGAG988200.042.3238078
GGTATCA296800.042.0476531
GCAGAGT1029650.040.5849279
TACAACG8500.039.8996542
TATCACG7750.037.619032
TAACGCA8250.032.454264
ACAACGC11200.031.8747223
AGAGTAC1016000.031.12316510-11
AGTACTT722600.030.86680612-13
TAGTACT17300.029.919025
ATAGTAC16900.029.5737084
GACCGTT10250.029.0215347
Produced by FastQC (version 0.11.5)