FastQCFastQC Report
Fri 10 Feb 2017
SRR3560055.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560055.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060365
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT681113.3057734915900827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291351.4140698371405067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229911.1158702462913124No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95950.46569418525358375No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA78710.3820196906858736No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT55580.2697580283105178No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54170.2629145806689591No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48440.2351039742958165No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45710.22185389481960718No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37630.18263754237720015No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC34570.16778580494232817No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33660.16336911178359176No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28680.13919863713468245No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG27380.13288907547934467No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA26800.1300740402792709No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG26070.12653097873435046No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT25680.12463811023774915No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC24680.11978460127210469No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT21070.10226343390612828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6850.074.245721
TACAACG4650.065.2525562
TAACGCA5950.052.9929584
GTATCAA345800.048.949811
TAGTACT10400.048.6233445
ATAGTAC10450.047.8213924
ACAACGC6250.047.593673
GCATAGT11300.045.279362
TATCACG4200.043.912962
GTACAAC7800.043.7243461
GTATAAC7450.042.5659831
AGATTAC10150.041.6171762
ATAACGC7900.041.418543
TATCAAC419050.040.321142
ATCAACG426800.039.587053
AACGCAG450500.039.5117266
TCAACGC437400.039.457374
CAACGCA441100.039.423125
GGTTTCA13100.038.36641
AGTGTAC11600.036.9261253
Produced by FastQC (version 0.11.5)