FastQCFastQC Report
Fri 10 Feb 2017
SRR3560053.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560053.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2150382
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT788893.6686039968712536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347941.6180380974171098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262991.2229920079316141No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103600.4817748660470559No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59490.27664852105346865No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57140.2657202301730576No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55120.256326550352449No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44330.20614941903345546No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39560.1839673137144935No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39480.18359528679090506No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32200.1497408367443552No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC25270.11751400448850483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC23170.10774829774430775No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT21720.10100530975426691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA318800.058.540041
TATCAAC406100.045.7649042
CGCATAG4350.045.392141
ATCAACG417950.044.4673463
TCAACGC425850.044.103444
CAACGCA431150.043.7268645
TAACGCA3550.043.5711984
TACAACG4650.043.499122
AACGCAG437550.043.3999986
ACGCAGA478750.039.6153957
CGCAGAG485550.039.2847948
ACAACGC5550.038.5890433
GCAGAGT502750.037.8697859
GGTATCA164350.035.5333441
GTATAAC6250.031.5929281
GCATAGT6550.030.8810562
TTCAACG12550.030.81234
AGTACTT338400.030.65395212-13
TAGTACT6600.030.6471065
TATCACG3500.030.59562
Produced by FastQC (version 0.11.5)