FastQCFastQC Report
Fri 10 Feb 2017
SRR3560031.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560031.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390665
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1173012.1760024041560735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT523220.9706038123311316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374060.6939032568338044No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA164380.3049345488914633No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159230.29538099659318473No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT125110.23208639379371562No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG85680.15894142930417676No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85330.15829215875963354No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78790.14616007487016908No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC73060.13553058852664746No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62070.1151434934279908No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59130.10968962085382786No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA58340.1082241244818589No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG57660.1069626845667464No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC57110.10594240228246421No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA661000.046.039061
CGCATAG11700.039.3782541
TATCAAC801550.037.934852
TCAACGC821850.037.367024
CAACGCA826350.037.3215755
ATCAACG813900.037.2861333
AACGCAG840650.037.2104036
ACGCAGA912650.034.2617957
CGCAGAG924600.033.9378
GCAGAGT965500.032.4624069
TATCACG8650.031.637692
TAACGCA9200.031.0396234
AGTACTT548300.028.34818512-13
CATGGGG168400.027.6619454
GGACCGT16450.027.4856956
TAGTACT17800.026.7380055
GAGTACT508500.026.40168212-13
TACAACG10200.026.246742
ATAGTAC18500.025.7265344
GACCGTT11400.025.571157
Produced by FastQC (version 0.11.5)