FastQCFastQC Report
Fri 10 Feb 2017
SRR3560025.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560025.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4410487
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT994512.254875708736926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480741.0899930098422237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326860.741097298325559No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA186680.4232639162069857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144620.3279002976315314No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT135690.3076531004399287No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC90360.20487533462857954No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80360.18220210149128657No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG78770.178597057422457No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72860.16519717663831682No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA72330.16399549528204027No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC65420.14832829118417082No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60660.13753583221081936No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG59190.1342028669396373No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54730.12409060496040461No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT53300.12084833262177169No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG51490.11674447742392167No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT51440.1166311112582352No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC48870.11080409034195089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA7950.053.881094
CGCATAG10050.048.2300951
TATCACG8400.042.4955022
GTATCAA625550.039.996071
GTATAAC13400.036.619151
TACAACG7750.036.080052
TCACGCA10400.034.8953444
TTCAACG24850.033.517584
TATCAAC745100.033.479682
ATCACGC11250.033.316473
ATAACGC15300.033.0520553
ATCAACG753900.032.9941863
AACGCAG783050.032.973976
GACCGTT8300.032.9724127
CAACGCA770700.032.892525
TCAACGC765650.032.8841324
TAGTACT16450.031.826425
GGTTTCA26150.031.3507561
ATAGTAC16550.030.9151574
ACGCAGA846000.030.4782167
Produced by FastQC (version 0.11.5)