FastQCFastQC Report
Fri 10 Feb 2017
SRR3560012.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3560012.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631130
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1342565.102598503304664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT623782.3707684530980986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT443441.6853595223345101No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212240.8066496144242208No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA145770.5540205159000124No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120400.45759806623009885No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT107480.4084936890233474No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102030.3877801552944932No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG87670.33320284440525555No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85450.32476540497808926No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71450.2715563275094731No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC61940.2354121613147203No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58620.22279400865787705No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50610.19235081504904736No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50310.19121062053186275No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA49560.18836013423890116No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT46690.17745227335783484No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40360.15339416904523911No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT38130.1449187231341667No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC38020.144500651811199No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC37580.1428283665193282No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.12986815550732955No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32140.1221528392743802No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT31340.11911232056188786No Hit
GTACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30570.11618582130111398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC29860.113487360943777No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA29710.1129172636851847No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC29510.1121571340070616No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29140.11075089410253389No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA28530.10843249858425846No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT28260.10740632351879231No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27580.10482188261317382No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC27490.10447982425801842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG5800.086.172732
CGCATAG11850.084.191851
TCACGCA6000.072.391944
TATCACG5850.072.213942
GTATAAC9300.066.807051
ACAACGC8050.064.304593
GGTTTCA16650.063.508371
GTACAAC8050.063.0804751
TAGTACT16600.060.928175
ATAGTAC16650.060.7509734
GCATAGT16600.060.2170872
AGATTAC18900.056.981692
TAACGCA11550.055.6365664
CATAGTA18250.055.098833
AAACGCA13250.052.983845
GTATCAC9050.052.8096541
ATCACGC8050.052.4784553
TCAAACG4850.051.5259633
GTATCAA642150.047.0280081
ATCTACG6500.046.6847882
Produced by FastQC (version 0.11.5)