FastQCFastQC Report
Fri 10 Feb 2017
SRR3559987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6302759
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1559382.4741228404893794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT739171.1727721145612582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT503710.7991896881984542No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA316550.5022403680673814No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT241840.38370497745511134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231130.36671241911677094No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC160920.25531675889876165No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG151690.24067237855675583No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA139460.22126817795190962No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127280.2019433076847774No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116520.184871419008723No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG109340.17347958251299153No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT95290.15118775761535544No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95040.15079110592678538No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG94180.14942662411810448No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC89840.14254075080452863No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC83930.13316390488673294No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83410.13233886937450726No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT82230.13046667340445667No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC69250.10987251773390033No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC65690.10422419768866302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG13650.056.43461
TACAACG11050.053.3082242
TAACGCA13100.050.415734
TATCACG10250.045.858992
GTATCAA1018450.037.7836761
ACAACGC15850.037.1644063
TCACGCA13150.037.1024444
AGATTAC25200.036.8337022
GTATAAC19550.036.3486751
TAGTACT23450.034.505345
GTACAAC20500.033.7903371
ATAGTAC23700.033.39014
GGTTTCA30400.032.0186041
ATCACGC14950.031.8397833
TATCAAC1206600.031.7425252
AACGCAG1259650.031.6551826
GCATAGT24700.031.5569932
TCAACGC1235600.031.5556144
CAACGCA1239750.031.5154255
ATCAACG1214700.031.5112633
Produced by FastQC (version 0.11.5)