FastQCFastQC Report
Fri 10 Feb 2017
SRR3559970.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559970.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3015839
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT735982.440382261785195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335861.113653613472072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239230.7932452627610427No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106540.35326819501969436No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA84120.27892735653328976No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64960.21539611365195555No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT64900.21519716403959227No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55300.1833652260614708No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT45790.15183171250189417No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44980.14914589273499018No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC43230.14334319570772844No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39440.13077621186011587No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36390.12066293989831686No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32690.10839438046924918No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC32130.10653751742052543No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT30240.10027060463108277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5850.062.731621
GTATCAA355450.051.0802731
TACAACG6450.045.1949272
TATCAAC443150.041.46872
ATCAACG452600.040.5317653
TCAACGC463400.040.2411164
AACGCAG478350.040.0901576
CAACGCA469400.039.9294975
CGTATCA3700.037.397161
CATGGGT48200.037.3924644
ACAACGC8100.036.718143
ACGCAGA525450.036.4739387
TAACGCA6400.036.247094
CGCAGAG531350.036.1267058
TATCACG5600.035.057362
GCAGAGT553350.034.7441379
GGACCGT10200.034.4064876
GACCGTT7350.033.9899447
ATAGTAC10850.033.989944
TAGTACT11250.032.7814145
Produced by FastQC (version 0.11.5)