FastQCFastQC Report
Fri 10 Feb 2017
SRR3559965.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559965.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3291290
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1236113.7557006523278105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT566231.720389269860754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388811.1813301167627284No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178290.5417024935511607No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100870.3064755764457097No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99610.30264729027220333No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80950.24595219503598895No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74550.22650693193246416No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69530.21125455368563692No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65040.19761248628957034No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45390.1379094519170295No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC40990.12454083353335622No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35640.1082858089077535No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35180.10688818062218766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA4050.059.431421
GTATCAA476850.058.742041
CGCATAG7650.055.8480531
TACAACG7350.050.1884962
TATCAAC602800.047.2191052
ATCAACG621100.045.787283
TATCACG6500.045.7674262
ACAACGC7800.045.7556043
TCAACGC634950.045.38744
CAACGCA639850.045.160675
AACGCAG649950.045.1178136
TAACGCA7200.041.3065154
ACGCAGA711550.041.1951757
CGCAGAG720550.040.7631768
TCACGCA7700.039.396764
GCAGAGT750400.039.0941059
TAGTACT12050.038.996095
GGTATCA238850.037.034221
ATAGTAC12250.036.417174
GTATAAC9300.034.9399221
Produced by FastQC (version 0.11.5)