FastQCFastQC Report
Fri 10 Feb 2017
SRR3559964.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559964.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6553673
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1717942.621339209325824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT828761.2645733163677835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT565220.862447668658476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244040.3723713404681619No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA158330.24158971617900374No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137200.2093482540248804No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT134240.20483170277186552No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT123050.18775730800117735No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC107730.16438110354300559No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107460.16396912082735896No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91000.1388534337920125No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC84330.12867593485363094No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC81720.12469343526904683No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80470.12278610788179392No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT78870.12034472882611019No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA74680.1139513674240384No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG74100.11306636751635304No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA72920.11126585046278628No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT68700.10482671320342044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA815750.050.5554161
TATCAAC1011250.041.145222
ATCAACG1032600.040.2486463
TCAACGC1045600.040.1293874
CAACGCA1049800.040.02555
AACGCAG1062900.039.890366
CGTATCA9650.039.2705731
CGCATAG9450.037.5555531
ACGCAGA1156100.036.6436927
CGCAGAG1168600.036.3187338
GCAGAGT1209650.035.0518159
TAACGCA9000.033.7070164
TACTGGT54200.031.2822422
TACAACG8400.030.4537962
GTACTGG64750.030.0994951
GGACCGT25300.029.623936
CATGGGG188300.029.09394
AGTACTT743500.029.08611912-13
GACCGTT15500.028.7820847
GAGTACT688400.027.91028212-13
Produced by FastQC (version 0.11.5)