FastQCFastQC Report
Fri 10 Feb 2017
SRR3559959.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559959.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903533
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361191.8974717013048894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159200.8363395853920054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121890.6403356285391427No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51490.27049701791353237No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46790.2458060879427885No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41430.21764792099742952No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT33040.17357198430497395No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25220.13249047954514054No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.12655414957345104No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24040.1262914801056772No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23070.12119569243086409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4950.063.2055631
TAACGCA4650.053.724714
GTATCAA212250.045.213711
GTATAAC7200.042.618181
TACAACG3700.038.591352
TAGTACT8800.037.8514985
TATCACG2050.037.7285352
TATCAAC260400.037.378582
TCAACGC267250.037.346684
CAACGCA270100.037.1287845
ATCAACG263850.037.084973
AACGCAG275600.037.078466
ATAGTAC9550.034.878874
ACGCATA1902.5465852E-1134.8334351
ACAACGC4150.034.3995133
ACGCAGA300000.034.2213637
CGCAGAG305300.033.7831428
CGTATCA2153.6379788E-1233.581491
GCAGAGT317250.032.5106169
ATAACGC9950.031.0863293
Produced by FastQC (version 0.11.5)