FastQCFastQC Report
Fri 10 Feb 2017
SRR3559952.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559952.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3773661
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT463101.227190253708534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221080.5858501863309926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151270.400857416710192No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA92520.24517305608532403No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT71660.18989517076388154No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63020.1669996324524116No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45800.12136755262330136No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC45400.12030757399777033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG7050.050.6309242
TAACGCA7000.048.4358834
CGCATAG7950.043.8939931
GTATCAA339500.040.4585531
ACAACGC9050.037.464223
GTATAAC9350.037.3216321
TCAACGC417200.033.833264
ATCAACG410100.033.8098263
TATCAAC411350.033.726462
CAACGCA420200.033.6624875
AACGCAG431550.033.5490236
ATAACGC12250.032.0478023
ACGCAGA466650.031.0383137
CGCAGAG474350.030.7100338
GCAGAGT498000.029.2874499
GTACAAC16000.027.0742681
CATGGGG92850.026.2659034
AGTACTT274850.026.10008812-13
TATCACG5150.025.4137742
CGTATCA4600.024.8507791