FastQCFastQC Report
Fri 10 Feb 2017
SRR3559951.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559951.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4574067
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT634131.3863592291061761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241410.5277797636108086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192140.4200638075480748No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA139740.305504925922598No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT120640.26374777632247187No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC91090.1991444375432192No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG86890.18996223710758936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74930.16381482824803398No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT66540.14547228975876392No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC54610.1193904680451773No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG52470.11471191829940401No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG50830.11112648765311046No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50300.10996778140766193No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC48920.10695077269309784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA516050.044.5177351
TATCAAC618650.037.454142
ATCAACG630450.036.69373
CAACGCA637600.036.6740535
TCAACGC637300.036.6166464
AACGCAG648300.036.3715486
ACGCAGA706450.033.360867
CGCAGAG715450.033.0333758
GCAGAGT742450.031.8240689
CATGGGT69100.030.0435714
CGCATAG6450.029.8523961
CGTATCA5450.028.7055421
CATGGGG172650.027.8731544
GGACCGT14200.027.6477476
GAGTACT294250.027.59496312-13
AGTACTT327200.027.07951412-13
TATCACG6900.026.7269882
GTACTGG41400.026.161411
GACCGTT10950.026.0754187
TACTGGT39300.024.5222242
Produced by FastQC (version 0.11.5)