FastQCFastQC Report
Fri 10 Feb 2017
SRR3559949.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559949.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947400
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT978022.4776308456199017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473591.1997517353194507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331230.839109287125703No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130420.33039469017581197No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA94890.24038607691138472No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT80520.20398236814105486No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72470.18358919795308304No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG54930.1391548867609059No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC54820.13887622232355473No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51550.13059228859502459No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48710.12339767948523078No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT45720.11582307341541268No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43750.11083244667375995No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC42480.10761513907888737No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC41510.10515782540406343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG7600.056.9858631
GTATCAA489000.055.231361
TATCAAC617100.044.1435172
ATCAACG631300.043.069743
TCAACGC645050.042.6214144
CAACGCA648200.042.4785275
AACGCAG659050.042.383916
TAACGCA6300.041.5435334
TATCACG7200.038.8344542
ACGCAGA720250.038.7990467
CGCAGAG731450.038.3518188
GCAGAGT760900.036.875269
ACAACGC9150.035.105043
TAGTACT13150.034.8302235
ATAGTAC13000.033.8594324
AGATTAC17900.033.567592
TACAACG10050.032.5573042
TCACGCA8900.032.0806054
GGTATCA260850.030.8772031
GCATAGT14650.030.05012