FastQCFastQC Report
Fri 10 Feb 2017
SRR3559946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5575656
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140362.0452481286506914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532370.9548114159123159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360190.6460047033030731No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164530.29508635396444827No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA114610.20555428814116222No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT92670.16620465825007855No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91170.16351439184913846No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89590.16068064457348158No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67450.1209723124956059No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61720.11069549484401477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA521900.051.9127541
TATCAAC657550.041.696872
ATCAACG666950.041.0319483
TCAACGC678200.040.9827584
AACGCAG695400.040.7730946
CAACGCA682950.040.7412645
TATCACG8700.040.342082
ACGCAGA764750.037.0212147
CGCATAG10450.036.8569641
CGCAGAG775900.036.558528
GCAGAGT817500.034.729
TAACGCA9000.034.3656044
CGTATCA7050.031.5840971
GGTATCA290450.030.499471
TACAACG9600.030.3633962
CATGGGG143450.029.8949554
TCACGCA12350.029.8598734
CATGGGT64900.029.3270574
AGTACTT531650.028.2489712-13
AGAGTAC800600.027.2211810-11