FastQCFastQC Report
Fri 10 Feb 2017
SRR3559937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408100
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291832.072509054754634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143861.021660393437966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103850.7375186421418933No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70880.5033733399616505No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49330.3503302322278247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46990.3337120943114836No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.2523968468148569No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32510.23087848874369718No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31840.22612030395568497No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT31260.22200127831830127No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG26460.18791279028478092No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA23800.16902208649953837No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22180.15751722178822528No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21440.15226191321639088No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18640.132376961863504No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC17210.12222143313685106No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT16850.11966479653433705No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16170.11483559406292167No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC16040.11391236417868048No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14890.10574533058731624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3700.058.5435751
TATCACG3400.054.2333762
TCACGCA3800.050.086354
TACAACG3250.047.585412
ATCACGC4300.044.2623523
GTATCAC5050.044.0847971
GTATAAC4450.041.916031
TAACGCA4050.041.1202744
TAGTACT6050.039.3239945
CGTATCA1401.6971171E-938.6805761
ACAACGC4250.037.7857323
TACTGGT9350.037.533952
AGATTAC7200.036.3499682
GGTTTCA7450.036.344171
GTATCAA227450.034.628381
AAACGCA4850.034.3375475
GTACTGG11950.034.2388761
ATAGTAC7100.032.670764
GTACAAC5900.032.634461
GGTTCAC10150.032.229216
Produced by FastQC (version 0.11.5)