FastQCFastQC Report
Fri 10 Feb 2017
SRR3559908.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559908.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4948857
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1331412.690338395310271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT637281.2877316923887678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453900.9171814825120225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199280.40267884079091393No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA108440.2191213041718522No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101320.2047341436618597No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88550.17893020549997707No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT87470.17674788340014674No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74450.1504387780855256No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73910.14934761703561045No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC67730.1368598850199147No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG62060.1254026939958055No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT53840.10879279801376358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG11900.062.345491
GTATCAA617250.058.295181
TATCACG10200.046.0821462
TATCAAC793850.045.2918322
TAACGCA11250.044.95444
ATCAACG806650.044.4920043
TAGTACT17000.044.4475065
AGATTAC22400.044.358232
TCAACGC830400.044.0506444
CAACGCA841500.043.5813945
AACGCAG859600.043.4250876
ATAGTAC16950.043.1758774
GTATAAC14300.042.2586481
TACAACG9550.041.742382
ACGCAGA943700.039.6245087
CGCAGAG952550.039.400828
GGTATCA315050.038.4950181
TCACGCA12600.038.2490164
GCATAGT19500.038.140042
GCAGAGT992100.037.680189
Produced by FastQC (version 0.11.5)