FastQCFastQC Report
Fri 10 Feb 2017
SRR3559891.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559891.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5287234
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1973393.732367434465734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT986381.8655879425801845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT646451.2226619816713238No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343710.6500752567410484No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229750.43453722683732177No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175320.33159114955002933No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA165300.31263984155042124No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160310.30320201451269224No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142460.26944145086069576No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT134560.2544998008410447No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG88450.1672897397769798No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC66140.12509376358224356No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62980.11911710357438313No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC62270.11777424642071828No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG57850.10941448780212867No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG57100.1079959767243137No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA56850.10752313969837536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG11000.065.837391
GTATCAA841100.054.01711
TACAACG10950.053.249212
TATCACG8600.052.5794872
AGATTAC26400.043.2711562
TCACGCA10450.043.270754
TATCAAC1065100.042.588632
GTACAAC15650.042.451061
ATCAACG1086950.041.710223
TCAACGC1113550.041.306944
TAGTACT18050.041.202325
CAACGCA1128200.040.8488925
AACGCAG1150100.040.790126
GGTTTCA27000.039.9014471
GATTACT29150.038.9844933
ATAGTAC19000.038.2035144
ACGCAGA1256450.037.337517
CGCAGAG1266050.037.1111348
TAACGCA12200.036.5761954
GTATCAC16150.036.3189661