FastQCFastQC Report
Fri 10 Feb 2017
SRR3559887.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559887.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6839043
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010682.940001985657935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT933991.3656735306387164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT633100.9257143141226045No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336340.49179395421259964No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA221700.3241681621244376No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210200.3073529439718393No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT168030.24569227010270298No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159640.23342447181572043No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153180.2239787057926087No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127690.18670740920915396No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT114870.16796209645121402No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT103970.15202419402831654No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC99690.14576600848978433No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG84420.12343832316889952No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG83700.12238554429325858No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC77830.1138024720710193No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA74100.1083484926180461No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT71100.10396191396954221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA887550.052.8840521
TATCAAC1104600.042.450732
ATCAACG1135200.041.3252943
TCAACGC1157250.040.887494
CAACGCA1178050.040.200925
AACGCAG1200600.039.926836
CGCATAG13350.036.7265851
ACGCAGA1311650.036.5600627
CGCAGAG1324050.036.2546778
GCAGAGT1356400.035.284999
GACCGTT18050.034.2804077
TACAACG11100.033.769642
TATCACG10400.032.609972
GTACTGG62550.031.354111
GGACCGT27600.031.2571076
TACTGGT55500.030.660692
TTAGGAC24450.030.6604373
GGTATCA496600.030.0649011
TAACGCA10500.030.0312354
AGTACTT831050.029.86188312-13
Produced by FastQC (version 0.11.5)