FastQCFastQC Report
Fri 10 Feb 2017
SRR3559867.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559867.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5911219
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT906771.5339814004522587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT428720.7252649580399575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297350.5030265331059466No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA214510.36288623378697354No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT155660.26332978020269593No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139170.23543367281773864No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG103950.17585205352736888No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC91350.15453665309980902No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81520.13790725736941908No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG75420.12758789684496546No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT72010.12181920514195127No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA71660.12122711068563016No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69060.11682869472438764No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG66030.11170284843109349No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60860.10295676746200741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG12000.053.804841
TAACGCA11950.047.298824
TATCACG10100.043.00312
TACAACG12950.040.8901332
GTATCAA723100.037.5184561
GTACAAC17650.032.855331
AGATTAC23500.032.6602972
TCACGCA14500.032.005254
GTATAAC20050.031.9042261
AACGCAG921300.031.1789046
TCAACGC891000.031.1507554
CAACGCA900400.030.9973495
TATCAAC872650.030.9947152
ATCAACG873450.030.8502643
CATGGGT100900.030.7803764
GTACTGG41350.030.5060351
ACAACGC17850.030.3317723
TAGTACT22550.030.0782745
GTATCAC20850.029.5331631
ATCACGC15750.029.4651493
Produced by FastQC (version 0.11.5)