FastQCFastQC Report
Fri 10 Feb 2017
SRR3559856.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559856.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3487564
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670841.9235202565458298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324560.9306209147703095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229680.6585685596020604No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160290.4596044689072373No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126950.3640076569204178No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81720.23431828061076443No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA67530.1936308552330509No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58350.16730875763140116No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48850.14006911414385514No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46740.1340190459587265No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40280.1154960883871952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8850.058.7796331
TAACGCA7900.057.2362864
GTATCAA317200.049.9909321
TACAACG8400.046.0395932
TATCACG6100.042.91612
GTATAAC11550.041.4151151
TATCAAC394050.039.9970552
ATCAACG397350.039.6337973
TCAACGC408200.039.3965344
CAACGCA415150.038.9949575
AACGCAG429700.038.6299176
ATAACGC14000.037.3972433
ACGCAGA469400.035.3500757
TAGTACT15600.035.087165
ACAACGC10900.034.9332053
CGCAGAG478150.034.9152158
ATAGTAC15500.034.1619954
GCAGAGT499950.033.297559
GCATAGT16850.032.4851842
GGTATCA182150.030.922361