FastQCFastQC Report
Fri 10 Feb 2017
SRR3559853.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559853.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6108117
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070843.3903083388874182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1037391.6983793859875311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT635831.0409591040905077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379340.6210424587479251No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283460.4640710058435357No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA217770.35652558718177796No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206580.33820570234656605No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167850.2747982725281785No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT167470.27417614954003006No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162510.26605580737893525No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG115350.1888470702182031No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT95870.15695508124680652No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC94600.15487588073378422No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC90140.14757412145183205No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81340.1331670627789219No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC79450.13007281949576277No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA74490.12195247733466795No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG73970.12110115114035963No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG73240.11990602013681138No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT63910.10463126361201006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG15600.063.274181
TACAACG12950.056.0523382
GTATCAA941600.049.1619871
TAACGCA13200.047.3261574
AGTGTAC26000.040.9605333
ATAACGC16300.040.8805123
AGATTAC30000.040.2604372
GTACAAC19750.039.9828761
TATCAAC1174500.039.462822
ATCAACG1200800.038.572153
TAGTACT26600.038.471255
TCAACGC1235850.038.248474
AACGCAG1277650.037.979986
CAACGCA1253850.037.823065
TCACGCA14350.037.7290084
GCATAGT27050.037.1726152
ACAACGC19900.037.0727233
GTATAAC17700.036.840071
ATAGTAC27750.036.447844
ACGCAGA1390700.034.8540887
Produced by FastQC (version 0.11.5)