FastQCFastQC Report
Fri 10 Feb 2017
SRR3559835.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559835.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375411
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT918693.8674991401487993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400401.6856030388004435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309261.3019220673811818No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142520.5999803823422557No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81000.3409936217353544No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61680.2596603282547736No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60310.25389290526986696No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50220.21141604547591977No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT46810.19706063498064122No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC34320.14448026046860943No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29850.1256624643061769No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT25910.1090758609773214No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC24910.1048660631781195No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA24850.10461347531016738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA307200.071.416431
GGTATCA123750.057.6951561
TATCAAC408050.053.877052
ATCAACG421250.052.1181763
TCAACGC428350.051.5459944
CAACGCA435450.050.6918755
AACGCAG443000.050.201886
GACCGTT5250.049.8629347
ACGCAGA492900.045.155787
CGCAGAG497450.044.7418148
GCAGAGT513200.043.2759559
TCTAACG1850.041.8094442
CATGGGT57750.036.4715354
GGACCGT10000.036.2922866
TTAGGAC8800.035.8340533
TACTGGT18650.035.411712
GTACTGG20800.034.182221
CGCATAG2700.033.192821
AGAGTAC509100.033.1419810-11
GGTTCAC21000.031.1643336
Produced by FastQC (version 0.11.5)