FastQCFastQC Report
Fri 10 Feb 2017
SRR3559813.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559813.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4943013
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1141162.3086324069954904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543351.0992283451409088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341010.6898828710343266No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA254250.514362393948792No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211350.42757322305241763No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197560.39967525879458543No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT180880.3659306580824287No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133290.26965334705775607No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG127020.2569687759267475No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC115630.23392614990088031No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA95220.19263554435321129No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG90000.18207518369868742No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89850.18177172505918962No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86510.17501471268637164No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG76760.15528990111901383No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT73330.1483508135624972No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63890.12925314985010153No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC62140.1257127990559604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG13800.066.251421
TACAACG10950.055.4194872
TATCACG8400.053.120052
TAACGCA11950.049.786154
TAGTACT22750.040.2731675
GCATAGT22700.039.8376922
ATAGTAC22800.039.662974
TCACGCA11700.039.6629684
AGATTAC25350.037.550742
GGTTTCA28200.036.870841
GTATAAC17200.036.8264851
GTATCAA769000.036.0324331
GTACAAC18600.035.982221
ACAACGC16750.035.1638833
GTATCAC18300.034.9394071
ATCACGC13750.033.749583
GATTACT28500.033.191643
CATGGGT76800.032.4585654
CATGGGG134500.032.1137354
TATCAAC907050.030.4736482