FastQCFastQC Report
Fri 10 Feb 2017
SRR3559796.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3559796.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081699
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT515971.6743036876735853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247340.8026092100493916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171260.5557324060526353No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100030.32459367381434723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88390.28682230159402333No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72850.23639557270194134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63430.20582801889477198No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54550.17701274524215377No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT45850.14878156497438588No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38800.12590457406774638No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT35750.1160074361577818No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34220.11104264238655365No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT33870.1099069052493446No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31920.10357922691346559No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31830.10328718022104041No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA6500.054.0043954
CGCATAG6450.046.2804071
GTATCAA302800.042.706051
TATCACG4500.039.6648862
TACAACG6750.037.020562
TATCAAC374300.034.604782
TCAACGC382500.034.577894
ATCAACG376150.034.3865743
CAACGCA388400.034.2364545
AACGCAG400250.033.936346
ACAACGC7750.033.7785843
TTAGGAC9350.031.816243
GACCGTT7400.031.3561927
ACGCAGA435950.031.10277
CGCAGAG441750.030.815558
ATAACGC14900.030.3471413
GCAGAGT456100.029.7677489
GTATAAC11150.029.449281
CATGGGG92500.028.6868974
GTACTGG27350.028.1591281
Produced by FastQC (version 0.11.5)